Puromycin (10 mg/mL)
Cat No.: DCP-PM100MG
Size: 10 mL (10 x 1mL)
Sterile; Ultrapure, Filtered 0.1-micron membrane Once.
Description:
Puromycin (10 mg/mL) in PBS is a ready-to-use selection antibiotic solution for cell culture and molecular biology workflows. It is a protein synthesis inhibitor that acts by entering the ribosome and causing premature chain termination, making it useful for selecting cells that carry puromycin resistance genes such as pac.
Puromycin is an aminonucleoside antibiotic originally derived from Streptomyces alboniger. In cell culture, it is commonly used as a rapid selection agent because non-resistant cells are quickly eliminated after exposure. The PBS formulation provides a convenient aqueous buffer system for direct dilution into culture media, and puromycin solutions are reported to be soluble in PBS at least up to 10 mg/mL.
Applications
This product is commonly used to maintain selection pressure in cells carrying a puromycin-resistance marker, especially in stable transfection and gene-editing workflows. It is also used in research on protein synthesis, translational control, and antibiotic sensitivity assays. Because puromycin acts quickly, it is often favored when a fast kill curve is needed for selection optimization
Quality Control:
- Appearance: Clear, Colorless Liquid.
- pH: 7.4
- Sterility: Filtered 0.1-micron membrane Once.
- DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: -20 ℃/-80 ℃ in the dark.
- Shelf life: 24 months
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
