pET Expression vector pETIA
Product#: FNK-DV215
$310.96
Availability:
Ships in 24 hours
pET Expression vector pETIA
Cat No.: FNK-DV215General Information:
| Size: |
15 μg (lyophilized plasmid contains salt of TE buffer) |
| Storage: |
Store at -20ºC |
| Plasmid copy number: |
Medium copy |
| Replicon: |
pMB1 |
| Antibiotic resistance: |
Ampicillin |
| Feature and recommendation: |
Stringent regulation with lac repressor |
Description:
pETIA is a medium copy number, ampicillin resistant, stringent controllable T7 bacterial expression vector. The T7 expression system is one of the strongest expression systems and has been widely used with a coupling of BL21 (DE3) E. coli cell. T7 RNA polymerase gene is integrated in a genome of BL21(DE3) under control of lacUV5 promoter. Upon addition of isopropyl-1-thio-β-D-galactopyranoside (IPTG), T7 RNA polymerase is expressed in the BL21(DE3) cells harboring pETIA vector, and it induces a high-level protein expression from T7 promoter of pETIA. The pETIA has a lacI gene, which represses T7 RNA polymerase gene in the absence of IPTG. The regulation with lac repressor is beneficial to repress a basal level protein expression and to maintain a recombinant plasmid in BL21 (DE3) cell.
Plasmid Map:
Reconstitution: Resuspend the lyophilized pETIA with 15 µl of sterile water to make 1 μg/µl plasmid in 1 × TE buffer. After reconstitution, store at - 20ºC.
Reference:
1) Studier, F.W. and Moffatt, B.A., J. Mol. Biol. 189 (1986) 113–130.
2) Moffatt, B.A. and Studier, F.W., Cell 49 (1987) 221-227
3) Pan, S. and Malcom, B.A., BioTechniques 29 (2000), 1234–1238
General reference in this Product Information
Sambrook, J. and Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring
Harbor Laboratory Press, Cold Spring Harbor, NY.
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