Flamma® 648 Carboxylic acid
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 648 Carboxylic acid
Cat. No. List below
Description
Key features:
1. Excitation/Emission maxima: 648/663 nm
2. High extinction coefficient: ≥ 250,000 cm?¹M?¹
3. Low CF280: 0.025
4. Carboxylic acid functional group
5. Blue solid appearance
6. Molecular weight: 684.86 g/mol
7. Soluble in DMF and DMSO
Applications:
1. Custom fluorophore development
2. Reference standard for spectroscopic measurements
3. Precursor for synthesizing reactive dye derivatives
4. Basis for developing novel bioconjugation strategies
5. Calibration of fluorescence instruments
6. Deep-tissue and whole-body imaging
7. 3D cell culture imaging (spheroids and organoids)
8. Live-cell imaging studies
Advantages:
1. Structural advantage: Fluorophore attached to octanoic acid provides a stable platform for custom modifications
2. Excellent optical properties in the far-red spectrum
3. Minimal interference with protein absorbance due to low CF280 value
4. Versatile modification options through amide bond coupling or conversion to reactive amine
5. Enhanced tissue penetration and reduced autofluorescence
6. Reduced phototoxicity and photobleaching in live-cell imaging
7. Compatibility with the "near-infrared optical window" (650-950 nm) for improved in vivo imaging
- Fluorophore: Flamma® Fluors 648
- Functional group: Carboxylic acid
- Excitation/Emission Max.(nm): 648/663
- Spectrally similar dyes: Alexa647, DyLight650, Cy5
- Extinction coefficient: ≥ 250,000 cm-1M-1
- CF280: 0.025
- Appearance: Blue Solid
- Molecular Weight: 684.86 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWC1101 | Flamma® 552 Carboxylic acid | 550 | 565 | Alexa555, DyLight549, Cy3, ATTO550 |
| PWC1201 | Flamma® 648 Carboxylic acid | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWC1501 | Flamma® 675 Carboxylic acid | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWC1308 | Flamma® 749 Carboxylic acid | 749 | 774 | Alexa750, DyLight755, Cy7, IRDye750 |
| PWC1603 | Flamma® 774 Carboxylic acid | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Flexibility: Ideal starting point for custom labeling strategies
- Brightness: High extinction coefficient for superior sensitivity
- Stability: Generates stable fluorescence signals for reliable results
- Versatility: Suitable for various modification and application scenarios
- Quality Control: Excellent reference standard for dye-conjugates
| Feature | Flamma® Fluors 648 Carboxylic Acid | Reactive Dyes (e.g., NHS esters) |
| Reactivity | Inactive, requires activation | Ready to use |
| Customization Potential | High | Limited |
| Stability in Storage | Excellent | Good, but may hydrolyze |
| Application as Reference Standard |
Ideal | Less suitable |
| Flexibility in Coupling Strategies | High | Moderate |
Flamma® Fluors Carboxylic acid
Flamma® Fluors Carboxylic acids are non-reactive form of Flamma® Fluors that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid can be converted to a reactive amine form by using standard chemical techniques or coupled to hydrazines. Flamma® Fluors carboxylic acids can be coupled with amines at small molecules or peptides by standard amide bond coupling conditions.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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