The EZgeneTM blood gDNA kit simplifies the purification of nucleic acids from blood samples. The system utilizes the reversible nucleic acidbinding properties of our ezBind membrane and the speed and versatility of spin column technology to yield high quality gDNA with the OD260/280 ratio of 1.8-2.0. Up to 250 μL of fresh, frozen or anticoagulated whole blood can be readily processed at one time. This DNA Kit can also be used for the preparation of genomic DNA from buffy coat, serum, plasma, saliva, buccal swab and other body fluids. The EZgeneTM Blood DNA Kit allows for the single or multiple simultaneous processing of samples. Purified DNA is ready for applications such as PCR, Southern Blotting, and Restriction Digestion.
Benefits of the EZgeneTM Blood DNA Kit:
Reliability- Optimized buffers that guarantee pure DNA Safety- No organic extractions P Quality- Purified DNA can be directly used for most downstream applications
Storage and Stability:
All EZgene™ Tissue DNA Kit components are guaranteed for at least 12 months from the date of purchase when stored as follows: reconstituted Proteinase K -20°C, and all other materials at RT (22- 25°C). Binding Capacity Each ezBindTM DNA mini column can bind approximately 100 µg DNA. Using greater than 30 mg tissue or 1x107 cells is not recommended.
Kit Contents:
Catalog#
BMG-GD2311-00
BMG-GD2311-01
BMG-GD2311-02
Preps
4
50
250
DNA Mini Columns
4
50
250
Buffer TL*
2 mL
20 mL
60 mL
Buffer BL
2 mL
20 mL
60 mL
Buffer KB
2 mL
30 mL
110 mL
DNA Wash Buffer
1.25 mL
12 mL
50 mL
Elution Buffer
500µl
30 mL
80 mL
RNase A
10µl
110µl
500µl
Proteinase K
150µl
1.5 mL
6 mL
User Menu
1
1
1
* Buffer TL: For tissue genomic DNA kit.
Before Starting:
It is strongly advised that you familiarize yourself with the entire booklet before starting. EZgeneTM Kits are designed to be simple, fast, and reliable provided that all steps are followed diligently.
Materials supplied by users:
Tabletop microcentrifuge
Sterile 1.5mL centrifuge tubes
Water bath
RNase stock solution
Absolute ethanol
PBS Buffer
Important Notes:
Dilute DNA Wash Buffer with absolute ethanol as follows:
GD2311-01: Add 8 ml absolute ethanol (96-100%) to each bottle
GD2311-01: Add 48 ml absolute ethanol (96-100%) to each bottle
GD2311-02: Add 200 ml absolute ethanol (96-100%) to each bottle
Preheat Elution Buffer to 65° C (in aliquots of 0.5 ml/ sample)
For Mouse Tail Protocol have the following ready: Pre-mixed solutions of 200 μl of Buffer BL and 210μl of absolute ethanol (per sample). Mix by vortexing. They can be prepared fresh, or premade and stored at room temperature. Do not store this mixture for more than one month
