Applications:

• It is specifically designed to extract genomic DNA from a variety of plant tissues such as leaves and cultured cells. This makes it suitable for a wide range of plant species, including tobacco, rice, Arabidopsis, tomato, pumpkin, and maize.
• The purified DNA can be directly used in PCR experiments, which is essential for genetic analysis and molecular biology research. The kit ensures low polysaccharide contamination, which is important for the success of PCR reactions.
• The kit is suitable for high-throughput extraction processes, making it efficient for laboratories that need to process multiple samples simultaneously.

Features:

• The kit ensures that the purified genomic DNA has low levels of polysaccharide contamination, which is crucial for the integrity of the DNA and its suitability for downstream applications such as PCR.
• It is suitable for high-throughput extraction, allowing for the processing of multiple samples simultaneously. This makes it efficient and ideal for large-scale studies or laboratories handling numerous samples.
• The kit utilizes magnetic silica beads that bind DNA in the presence of chaotropic agents, providing a simple and reliable method for DNA purification.
• The purified DNA can be used directly in PCR experiments, which streamlines the workflow and reduces the time needed for sample preparation.

*The reaction yield depends on the types of species, tissues, and sample conditions that are used. Table 2 shows typical yields from various plant tissues.

Components:

Principle:

The selectivity of extracted nucleic acids can be changed by optimization of the binding and washing solutions. MagExtractor -Genome- (Code No. NPK-101) extracts genomic DNA from various specimens (e.g. whole blood, cultured cells or animal tissues etc.). MagExtractor -RNA- (Code No. NPK-201) extracts total RNA from various specimens (e.g. cultured cells or animal tissues). MagExtractor -Plasmid- (Code No. NPK-301) extracts plasmids from E. coli cells, MagExtractor -Viral RNA- (Code No. NPK-401) is a kit for extracting viral RNA from serum or plasma specimens. MagExtractor -Plant Genome- (Code No. NPK-501) is a kit for extracting genomic DNA from various plant specimens (e.g., leaf, cultured cells, etc.). MagExtractor-PCR & Gel Clean up- (Code No. NPK-601) extracts DNA fragments from a PCR solution, enzyme solution, or agarose gel slices.

Application Data:

	Fig. 1. Electrophoretic analysis of purified genomic DNA M: λ/ Hind III Marker 1,4,7: 5mg 2,5,8: 10mg 3,6,9: 20mg

	Fig. 1. Electrophoretic analysis of the PCR products M: λ/ Hind III Marker 1,2: 1.3kb rbcL gene (chloroplast genomic DNA) 3,4: 2.2kb rbcL-Methyltransferase (genomic DNA) 5,6: 4.6kb rbcL-Methyltransferase (genomic DNA)

References:

1. B. Vogelstein and D. Gillespie, Proc. Natl. Acad. Sci. USA. 76: 615-619 (1979)