Product Name: AllView PAGE Buffer / DynaMarker Protein MultiColor Stable II set
Code No: FNK-DS522 Components
Components
AllView PAGE Buffer
DynaMarker Protein MulitColor Stable II
Code No
FNK-DS520S
FNK-DM660S
Lot#
Size
50 mL (20x stock solution)
100 μL
Storage
Room temperature* (Protect from light)
4°C
Stability
12 months at RT
12 months at 4°C
*The storage condition of AllView PAGE Buffer (DS520S) is room temperature. Please transfer AllView PAGE Buffer to room temperature after receiving this set.
AllView PAGE Buffer
Description
AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer has a remarkable feature that enable us to separate proteins with wide range of molecular weights in the basic Laemmli gel (Tris-HCl) similar to using “gradient gel”. The recommended acrylamide concentration is 6% for resolving gel and 3% for stacking gel to separate the entire range of about 10 kDa to 250 kDa. After electrophoresis, the polyacrylamide gel can be used directly for CBB staining, silver staining or western blotting.
Protocol
1. Dilute the AllView PAGE Buffer 20 times with ultrapure water. (e.g. Add 25 ml of AllView PAGE Buffer to 475 ml of ultrapure water.)
2. Set the gel in electrophoresis chambers.
3. Fill the chambers with the 1 × AllView PAGE Buffer.
4. Load your samples and molecular weight markers.
5. Start electrophoresis.
Gel
Voltage
Time
Mini gel
(8 × 10 cm, 1 mm thick)
250 V
(Constant voltage)
13~15 min
Note:
AllView PAGE Buffer is suitable for Laemmli gels (see below “Recommended usage”).
If precast gels are used, the optimal acrylamide concentration may be different.
AllView PAGE Buffer is not reusable.
AllView PAGE Buffer is 20 × stock solution. Please dilute to 1 × solution before use.
If precipitation of SDS is observed, completely dissolve it in a water bath (about 37°C) before use.
Optimal electrophoretic time is depending on acrylamide concentration, gel size and voltage. Accordingly, it is recommended to monitor electrophoresis using a prestained MW Marker (e.g. DynaMarker® Protein MultiColor Stable II, Code#DM660).
Recommended usage
The recommended acrylamide concentration is 6% for resolving gel and 3% for stacking gel to separate the entire range of about 10 kDa to 250 kDa. Especially if the separation of low molecular weight proteins (10 kDa~30 kDa) is required, 10% resolving gel is recommended.
Description
The DynaMarker® Protein MultiColor Stable II is a pre-stained protein molecular weight marker. The marker has a remarkable feature that it is possible to store at 4 °C. The feature allow us to start electrophoresis with the marker, because it is always in a liquid state while stored at 4 °C. The DynaMarker® Protein MultiColor Stable II consists of nine prestained proteins. Each of them are stained red, blue, purple, green or orange, ranging in apparent molecular weight from approximately 8 kDa to 230 kDa. The DynaMarker® Protein MultiColor Stable II is suitable for visualizing proteins during electrophoresis without staining and for monitoring electrophoretic transfer onto membranes. The protein concentrations are optimized to give uniform band intensities. The marker is supplied in gel loading buffer for direct loading onto SDS-PAGE without heating or adding reducing agents.
