TDzyme® is a tissue dissociation enzyme produced from E. coli by using the manufacturing method of genetic recombination.
TDzyme® is manufactured by genetic recombination of collagenase derived from Clostridium histolyticum.
TDzyme® is manufactured in animal source free condition, so there is no risk of contamination with viruses.
TDzyme® is prepared by the purification of each enzyme with high purity and then mixing them by using column chromatography, which lead to high quality and lot-to-lot consistency.
TDzyme® is suitable for tissue dissociation and cell culture due to low endotoxin specification.
TDzyme® is manufactured in lyophilized form, which secures enhanced stability in storage.
TDzyme® is contained in a smart device enabling simultaneous dissolution and in-line filtration of lyophilized enzyme, which provides easy handling and contributes to reducing any contamination risks.
Application
TDzyme® consists of TDzyme C and TDzyme T.
TDzyme C is collagenase formulated to contain minimal amount of thermolysin. It is suitable for breakdown of various tissues including pancreatic islets.
TDzyme T is thermolysin and can be mixed with TDzyme C to dissociate stronger tissues such as adipose tissues, cartilage, and extracellular matrices of skin and blood vessels.
Storage and Stability
Please store the eluted enzyme solution immediately on ice.
Freeze unused enzyme solution as needed.
Please keep in mind that repeated freezing and thawing of the enzyme solution may result in decreased enzyme activity.
Recombinant tissue dissociation enzyme
Genetic engineering is used to secure animal source free condition.
- Collagenase class I (collagenase G) originating from Clostridium histolyticum
- Collagenase class II (collagenase H) originating from Clostridium histolyticum
- Thermolysin originating from Geobacillus stearothermophilus
Purified & blended type enzyme (not crude type)
- Highly purified using column chromatography technology
- Blended with three enzymes at fixed ratio
- High lot-to-lot consistency
- High purity & low endotoxin
Application on target tissues
Tissue Type
TDzyme TL Pack
TDzyme TM Pack
Adipose
√
Cornea
√
Heart
√
Kidney
√
Liver
√
Lung
√
√
Lymph Node
√
√
Ovarian
√
Pancrease (islets)
√
Skin
Spleen
√
Innovative packaging technology
Application of smart packaging technology providing higher user-friendly experiences than conventional vial packaging
Easy in-line sterile filtration to minimize microbial contamination during primary cell culture
Ordering information
Product name
Product No.
Contents
TDzyme® TL Pack
TDP0010
TDzyme® C (5mg), 2ea
TDzyme® TM Pack
TDP0008
TDzyme® C (5mg) + TDzyme® T (3mg)
TDzyme® C (blue cap): Collagenase (including minimal amount of thermolysin)
TDzyme® T (red cap): Thermolysin
1. Easy dissolution of lyophilized proteins
2 Easy dissolution of lyophilized proteins with in line sterile filtration
3 Simultaneous dissolution of lyophilized proteins /w in line sterile filtration