Anti-Cry j 2 mAb T27
Anti-Cry j 2 mAb T27
General information
| Cat. No. | ：FNK-HBL-Ab-2-T27 |
| Quantity | ：100 µg, 200 µl/vial (Frozen Liquid) |
| Specificity | ：This antibody recognizes Cry j 2 antigen (Japanese cedar pollen allergen). This antibody cross-reacts against Cry j 1 antigen less than 0.1%. |
| Isotype | ：Mouse monoclonal antibody IgG1,κ |
| Preparation & form |
：The hybridoma was inoculated in mice intraperitoneally. IgG was purified from collected ascites by ProteinG Sepharose column chromatography. Purified IgG was prepared in the concentration of 500μg/ml with phosphate-buffered saline and frozen. No stabilizers or preservatives are contained. |
| Purification | ：Protein G Sepharose Affinity Chromatography Purification |
| Solvent | ：PBS (without stabilizers and preservatives) |
| Immune animals | ：Mouse-Mono |
| Class | ：IgG 1 , κ |
| Application | ：This antibody is suitable for western blotting and enzyme-linked immunosorbent assay. Dilute 1:200~500 for Western blotting. Dilute 1:50 as a capture antibody. |
| Storage | ：Store below -20°C in the dark. Avoid multiple freeze/thaw cycles by storage in appropriate aliquots. |
Description
Pollen, purified antigens (Cry j 1, Cry j 2) and antibodies (anti-Cry j 1 antibody, anti-Cry j 2 antibody) of Japanese Sugi (Cryptomeria japonica). It is used in many treatises and boasts a high track record. It is useful for studying Japanese cedar pollen allergy.
It is an antibody produced using Cry j 2 of Japanese Sugi ( Cryptomeria japonica ) as an immunogen.
* This product is for research use. It cannot be used for anything other than research.
Features
- Unlabeled products do not contain stabilizers and preservatives.
- By using an unlabeled mouse monoclonal antibody as a capture antibody and an HRP - labeled antibody as a detection antibody in combination, a Cry j 1 or Cry j 2 ELISA system can be constructed by the sandwich method.
What is Japanese cedar pollen?
Two main types of Sugi pollen allergens are known as the causative agents of Sugi pollinosis. One is Cry j 1, a basic protein with a molecular weight of about 40 kDa. Cry j 1 is localized in ubiquitous bodies (particle size of about 0.7 μm) adhering to the surface of Sugi pollen with a particle size of about 30 μm. The other is Cry j 2, a basic protein with a molecular weight of about 37 kDa. Cry j 2 is thought to be localized in the starch granules and endometrium inside Sugi pollen.
References
Wang Seiyaku, et al., "Aerosol Research", 23 , 120-126 (2008).
Hiroshi Yasue, "Ayumi of Medicine", 200 , 358-362 (2002).
References
- Kawashima, T. et al.:Antigenic analysis of Sugi basic protein by monoclonal antibodies:I. Distribution and characterization of B-cell-tropic epitopes of Cry j I molecules. Int. Arch. Allergy Immunol., 98(2), 110-117, 1992
- Kawashima, T. et al.:Antigenic analysis of Sugi basic protein by monoclonal antibodies:II. Detection of immunoreactive fragments in enzyme-cleaved Cry j I. Int. Arch. Allergy Immunol., 98(2), 118-126, 1992
- Sawatani, M et al. : Immunological and physiological properties of Cry j II, the second major allergen of Japanese cedar pollen. [Article in Japanese] Arerugi, 42(6):738-747, 1993
- Sawatani, M et al.: Enzyme-linked immunosorbent assay for the quantification of Cry j I and Cry j II.[Article in Japanese] Arerugi, 43(3):467-473, 1993
