Anti Human HNF4 α7 mouse monoclonal antibody (H6939)
Anti Human HNF4 α7 mouse monoclonal antibody (H6939)
Hepatocyte nuclear factor 4 gamma
DiagnoCine offers excellent HNF | Anti Human Hepatocyte Nuclear Factor Antibodies to researchers studying Transcriptional Factors, Insulin secretion, Cellular Differentiation & Proliferation, Regeneration, Metabolism Regulation, Regulation of Gene Expression, Glucose & Lipid Homeostasis, and Drug Metabolism.
Human diseases include liver cancer, type-2 diabetes, gastric cancer, alcoholic hepatitis, colon cancer, pancreatic cancer, atherosclerosis, and psychiatric disorder.
Anti- Human HNF | Hepatocyte Nuclear Factor Antibodies have excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunoprecipitation research with optimization.
General information
| Cat. No. | ：FNK-PP-H6939-00 |
| Size | ：100 ul |
| Antigen Species | ：Human |
| Host Species | ：Mouse |
| Cross Reactivity | ：Human/ Rat |
| Purification | ：Ammonium sulfate fractionation |
| Clone No | ：H6939 |
| Concentration | ：1 mg/mL |
| Ig Class | ：G1 |
| Nomenclature | ：NR2A1 |
| Application | ：ELISA, IHC, WB ：In order to obtain the best results, optimal working dilutions should be determined by each individual user. |
| Specificity |
：This antibody specifically recognizes human HNF4 alpha 1, 2, 4, 5, 7 ,8 and cross reacts with mouse and
rat HNF4 alpha 1, 2, 4, 5, 7 ,8. |
| Formulation | ：Physiological saline with 0.1% NaN3 as a preservative. |
| Storage | ：Store at 2 - 8 ºC up to one month. For long-term storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in a frost-free freezer is not recommended. |
| Form | ：Physiological saline with 0.1% NaN3 as a preservative |
| Genbank | ：AY68069 |
Recommended Antibody Dilutions
|
ELISA |
WB |
Non reducing Western Blot |
IP |
Chromatin immunoprecipitation |
Supershift Assay |
Immunohistochemistry |
|
3 |
1.0 |
3 |
Decide by use |
Decide by use |
Not tested |
10-20 |
(µg/mL)
Description
Hepatocyte nuclear factor 4 alpha (HNF4, HNF4a; NR2A1) is a member of orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.
Orign
Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with a synthetic peptide derived from human HNF4 alpha (1-16 a.a.)
Specificity
This antibody specifically recognizes human HNF4 alpha 7- 9 and cross reacts with mouse and rat HNF4 alpha 7- 9.
Notes
Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.
Reference
- Tanaka T, et al. J. Pathol. 208, 662-672, 2006
- Oshima T, et al. Pathology International: 57, 82-90, 2007
Aliases for HNF4A Gene
- Hepatocyte Nuclear Factor 4 Alpha 2 3 5
- NR2A1 2 3 4
- HNF4 2 3 4
- Nuclear Receptor Subfamily 2 Group A Member 1 3 4
- Hepatocyte Nuclear Factor 4-Alpha 3 4
- Transcription Factor HNF-4 3 4
- Transcription Factor 14 3 4
- TCF-14 3 4
- TCF14 3 4
- Hepatic Nuclear Factor 4 Alpha 3
- HNF4alpha10/11/12 3
- HNF-4-Alpha 4
- HNF4alpha 3
- HNF4a7 3
- HNF4a8 3
- HNF4a9 3
- NR2A21 3
- FRTS4 3
- MODY1 3
- HNF4A 5
- MODY 3
- TCF 3
