Anti Human HNF4 α7 mouse monoclonal antibody (H6939)

Product#: PP-H6939-00
$577.00
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Anti Human HNF4 α7 mouse monoclonal antibody (H6939)
Hepatocyte nuclear factor 4 gamma
 

DiagnoCine offers excellent  HNF | Anti Human Hepatocyte Nuclear Factor Antibodies  to researchers studying Transcriptional Factors, Insulin secretion, Cellular Differentiation & Proliferation, Regeneration, Metabolism Regulation, Regulation of Gene Expression, Glucose & Lipid Homeostasis, and Drug Metabolism.

Human diseases include liver cancer, type-2 diabetes, gastric cancer, alcoholic hepatitis, colon cancer, pancreatic cancer, atherosclerosis, and psychiatric disorder.

Anti- Human HNF | Hepatocyte Nuclear Factor Antibodies  have excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunoprecipitation research with optimization.


General information 

Cat. No. :PP-H6939-00
Size 100 ul
Antigen Species Human    
Host Species Mouse
Cross Reactivity :Human/ Rat 
Purification Ammonium sulfate fractionation
Clone No :H6939
Concentration 1 mg/mL
Ig Class G1
Nomenclature NR2A1
Application   ELISA, IHC, WB
In order to obtain the best results, optimal working dilutions should be determined by each individual user.
Specificity
This antibody specifically recognizes human HNF4 alpha 1, 2, 4, 5, 7 ,8 and cross reacts with mouse and
    rat HNF4 alpha 1, 2, 4, 5, 7 ,8.
Formulation Physiological saline with 0.1% NaN3 as a preservative.
Storage Store at 2 - 8 ºC up to one month. For long-term storage, the solution may be frozen in working aliquots.
    Repeated freezing and thawing is not recommended. Storage in a frost-free freezer is not recommended.
Form Physiological saline with 0.1% NaN3 as a preservative
Genbank AY68069


Recommended Antibody Dilutions
 

ELISA

WB

Non reducing Western Blot

IP

Chromatin immunoprecipitation

Supershift Assay

Immunohistochemistry

3

1.0

3

Decide by use

Decide by use

Not tested

10-20

                                                                  (µg/mL)

 

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Description 


Hepatocyte nuclear factor 4 alpha (HNF4, HNF4a; NR2A1) is a member of orphan nuclear receptor. HNF4a is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4a in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4a gene is alternatively spliced and may generate up to nine different isoforms, HNF4a1 through HNF4a9.

Orign

Produced in BALB/c mouse ascites after inoculation with hybridoma of mouse myeloma cells (NS-1) and spleen cells derived from a BALB/c mouse immunized with a synthetic peptide derived from human HNF4 alpha (1-16 a.a.)
 

Specificity

This antibody specifically recognizes human HNF4 alpha 7- 9 and cross reacts with mouse and rat HNF4 alpha 7- 9.

Notes

Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.


Reference 

  1. Tanaka T, et al. J. Pathol. 208, 662-672, 2006
  2. Oshima T, et al. Pathology International: 57, 82-90, 2007



Aliases for HNF4A Gene

  • Hepatocyte Nuclear Factor 4 Alpha 2 3 5
  • NR2A1 2 3 4
  • HNF4 2 3 4
  • Nuclear Receptor Subfamily 2 Group A Member 1 3 4
  • Hepatocyte Nuclear Factor 4-Alpha 3 4
  • Transcription Factor HNF-4 3 4
  • Transcription Factor 14 3 4
  • TCF-14 3 4
  • TCF14 3 4
  • Hepatic Nuclear Factor 4 Alpha 3
  • HNF4alpha10/11/12 3
  • HNF-4-Alpha 4
  • HNF4alpha 3
  • HNF4a7 3
  • HNF4a8 3
  • HNF4a9 3
  • NR2A21 3
  • FRTS4 3
  • MODY1 3
  • HNF4A 5
  • MODY 3
  • TCF 3



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