Adeno-assiciated viruses, belong to the replication deficient parvovirus family, are small single-stranded DNA viruses. AAVs are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally AAVs are purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the volume of cell lysate to be processed. The ViraTrapTM AAV purification maxi kit is designed for efficient purification of rAAV (Serotype 2) transfected cell line. Viral particles from 5-6 T75 flasks can be purified per column. Each column can be regenerated for purifying the same rAAV. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
