The ViraTrapTM retrovirus purification maxi kit is designed for fast and efficient purification of recombinant retroviruses from retroviral-transfected cell culture supernatant. Up to 1x107 viral particles can be purified from cell culture media of 5 to 6 T75 flasks. Traditionally the recombinant retrovirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed. Each column can be regenerated for purifying the same retrovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
The Guaranteed shelf life is 12 months from the date of purchase.
Kit Components
Catalog#
BMG-V1272-00
BMG-V1272-01
BMG-V1272-02
Notes
Preps
2
4
10
RV Maxi Columns
1
2
5
Store @4°C
*Centrifugal Filter
2
4
10
Store at RT
Press-On Cap
1
2
10
Store at RT
50 mL Collection Tube
2
4
10
Store at RT
10x RV Wash Buffer
20 mL
40 mL
80 mL
Store at RT
2x RV Elution Buffer
20 mL
40 mL
80 mL
Store at RT
Regeneration Buffer
15 mL
30 mL
50 mL
Store at RT
*Centrifugal Filter (CF01) can be purchased from Biomiga separately
