Adenovirus Purification Mini Kit (20 Preps)
Cat. No.: BMG-V1160-02
Size: 20 Preps
Description
The ViraTrapTM adenovirus purification mini kit is designed for fast and efficient purification of recombinant Adenovirus from adenovirus transfected cell culture supernatant. Up to 3 x1010 viral particles can be purified from cell culture media of 1 to 2 T75 flasks.
Traditionally, the recombinant adenovirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Kit Components
| Catalog# | BMG-V1160-00 | BMG-V1160-01 | BMG-V1160-02 | Notes |
| Preps | 2 | 10 | 20 | |
| AV Mini Column | 1 | 5 | 10 | Store at 4°C |
| Press-On cap | 2 | 5 | 10 | Store at RT |
| Centrifugal filter | 2 | 10 | 20 | Store at RT |
| 15 mL Collection Tube | 1 | 5 | 10 | Store at RT |
| 10x AV Wash Buffer | 5 mL | 25 mL | 50 mL | Store at RT |
| 2x AV Elution Buffer | 5 mL | 25 mL | 50 mL | Store at RT |
| Regeneration Buffer | 15 mL | 75 mL | 150 mL | Store at RT |
Materials required but not supplied
- ddH2O
- PBS
- 0.45 μM and 0.22 μM filters
- Rack holder for column















