Traditionally the recombinant lentivirus is purified by ultracentrifugation to separate the virus particles from cellular proteins and media components. The ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed, in addition, the ultracentrifugation also concentrates cellular debris, membrane fragments, and unwanted proteins from culture medium. The ViraTrapTM lentivirus purification maxi kit is designed for fast and efficient purification of recombinant lentiviruses from lentiviral-transfected cell culture supernatant. Viral particles can be purified from cell culture of 4 to 5 T75 flasks per column. The viruses are first applied to a purification column and then further purified and concentrated through a concentration unit Each column can be regenerated for purifying the same lentivirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
The Guaranteed shelf life is 12 months from the date of purchase. Store LV column at 4°C and all other components at room temperature.
