The ViraTrapTM adenovirus purification maxi kit is designed for fast and efficient purification of recombinant adenoviruses from retroviral-transfected cell culture supernatant. Up to 1x1011 viral particles can be purified from cell culture media of five to six T75 flasks.
Traditionally the recombinant adenovirus is purified by ultra centrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the amount of cell lysate to be processed.
Each column can be regenerated for purifying the same adenovirus. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Kit Components
Catalog#
BMG-V1260-00
BMG-V1260-01
BMG-V1260-02
Notes
Preps
1
4
10
AV Maxi Column
1
2
5
Store at 4°C
Centrifugal filter*
1
2
5
Store at RT
Press-On Cap
1
4
10
Store at RT
50 mL Collection Tube
1
4
10
Store at RT
10x AV Wash Buffer
10 mL
40 mL
80 mL
Store at RT
2x AV Elution Buffer
10 mL
40 mL
80 mL
Store at RT
Regeneration Buffer
10 mL
30 mL
50 mL
Store at RT
*Centrifugal filter can be purchased from Biomiga separately
