Adeno-Associated Virus Purification Mini Kit (20 Preps)
Cat. No.: BMG-V1169-020
Size: 20 Preps
Description
Adeno-assiciated viruses, belong to the replication deficient parvovirus family, are small single-stranded DNA viruses. AAVs are important gene delivery tools, which have been used in gene therapy and RNAi delivery. Traditionally AAVs are purified by ultracentrifugation using CsCl to separate the virus particles from cellular proteins and media components. The CsCl ultracentrifugation procedure is time consuming and limited to the volume of cell lysate to be processed. The ViraTrapTM AAV mini purification kit is designed for efficient purification of recombinant AAV2 and AAV-DJ from rAAV vector transfected cell line. Up to 1 x 1012 viral particles can be purified from cell lysate of 2 x T75 flasks. Each column can be regenerated for purifying the same rAAV. For optimized viral binding and recovery, each column can be regenerated only once.
Storage and Stability
All components are guaranteed for at least 12 months from the date of purchase when stored as follows: Mini column and desalting tube should be stored at 4 °C, and all other materials at RT (22-25°C).
Kit Components
Catalog#
BMG-V1169-00
BMG-V1169-01
BMG-V1169-02
Notes
Preps
2
10
20
AAV Mini Columns
1
5
10
Store at 4°C
Press-On Cap
1
5
10
Store at RT
15 mL Collection Tube
2
10
20
Store at RT
AAV Binding Buffer
30 m
200 m
400 mL
Store at RT
AAV Elution Buffer
10 mL
50 mL
100 mL
Store at RT
Regeneration Buffer
10 mL
30 mL
100 mL
Store at RT
100xNuclease Reaction Buffer
100 µL
500 µL
1000 µL
Store at 4°C
Nuclease (25 u/μL)
12 µL
55 µL
110 µL
Store at -20°C
Centrifugal Filter*
1
5
10
Store at RT
*Centrifugal filter can be purchased from Biomiga separately
