Flamma® 774 PEG4-Alkyne
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 774 PEG4-Alkyne
Cat. No. List below
Description
The PEG4 spacer between the dye and the alkyne group enhances solubility and reduces steric hindrance, potentially improving reaction efficiency and labeled biomolecule functionality.
Flamma® Fluors 774 PEG4-alkyne participates in click chemistry reactions:
-Reaction Type: Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC)
-Product: Forms 1,4-disubstituted 1,2,3-triazole
-Compatibility: Reacts inside living systems without interfering with native biochemical processes
-Pre-requisite: Azide functionality must be introduced onto the target biomolecule through chemical or genetic modification
Its applications include cellular imaging (provides high-contrast visualization of labeled structures in cells), nucleotide functionalization (useful for labeling nucleic acids), in vivo imaging (excellent for deep tissue imaging due to NIR properties), biomolecule labeling (versatile tool for tagging various biomolecules including proteins and glycans).
The PEG4 spacer may provide improved water solubility compared to non-PEGylated alternatives. It is suitable for both in vitro and in vivo applications due to its biocompatibility and NIR properties. It can be used in conjunction with azide-modified biomolecules for specific and efficient labeling. It is ideal for studying protein-protein interactions, tracking cellular processes, and developing novel imaging probes.
Flamma® Fluors 774 PEG4-alkyne represents a significant advancement in NIR fluorescent labeling technology, offering researchers a powerful and flexible tool for exploring complex biological systems with high specificity, sensitivity, and biocompatibility.
What are the advantages?
1. Enhanced Solubility: PEG4 spacer improves solubility in aqueous environments
2. Reduced Steric Hindrance: Spacer may improve reaction efficiency and preserve biomolecule function
3. High Sensitivity: Exceptionally high extinction coefficient ensures strong signal generation
4. Low Protein Interference: Low CF280 value minimizes interference with protein absorbance measurements
5. Bioorthogonality: Click chemistry reaction occurs without interfering with native biological processes
6. Versatility: Compatible with various imaging systems due to its spectral similarity to widely used NIR dyes
- Fluorophore: Flamma® Fluors 774
- Reactive group: PEG4-alkyne
- Excitation/Emission Max.(nm): 774/800
- Spectrally similar dyes: Cy7.5, DyLight800, IRDye800
- Extinction coefficient: ≥ 182,000 cm-1M-1
- CF280: 0.1
- Appearance: Green Solid
- Molecular Weight: 1143.34 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWG1001 | Flamma® 496 PEG4-Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWG1122 | Flamma® 552 PEG4-Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWG1415 | Flamma® 581 PEG4-Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWG1215 | Flamma® 648 PEG4-Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWG1515 | Flamma® 675 PEG4-Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWG1301 | Flamma® 749 PEG4-Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWG1603 | Flamma® 774 PEG4-Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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