Tyrode's Balanced Salts Solution w/ Sodium bicarbonate and Phenol red: 1X
Cat No.: DCP-TBSSBR1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
- World’s cleanest buffer for all cell and molecular biology experiments
[+] Sodium Bicarbonate, [+] Phenol Red
Description:
Tyrode's Balanced Salts Solution is a physiologically balanced, isotonic salt solution specifically formulated to maintain cellular integrity and function in various biological applications. This ready-to-use solution contains sodium bicarbonate for optimal pH buffering capacity and phenol red as a visual pH indicator, making it ideal for cell culture applications, tissue preservation, and physiological experiments requiring precise pH monitoring. This solution closely mimics the ionic composition of interstitial fluid, creating an environment that supports cellular function outside of living organisms.
Applications:
- Cell and tissue culture maintenance
- Physiological experiments requiring precise pH monitoring
- Isolated organ perfusion studies
- Electrophysiological recordings in cardiac and smooth muscle tissue
- Short-term preservation of isolated tissues and organs
- Washing of cells and tissues during experimental procedures
Advantages:
- Physiological Compatibility: Formulated to be isotonic with interstitial fluid, minimizing osmotic stress on cells
- Optimal pH Management: Bicarbonate buffer system maintains physiological pH (7.2-7.4) when properly gassed
- Visual pH Monitoring: Phenol red indicator provides immediate visual feedback of solution pH
- Metabolic Support: Contains glucose as an energy source to support cellular metabolism
- Complete Ionic Balance: Provides essential inorganic salts in proportions that maintain cellular homeostasis
- Exceptional Quality: Manufactured under strict quality control standards to ensure consistency and reliability
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Calcium chloride dihydrate |
265.000 |
| Magnesium chloride anhydrous |
46.900 |
| Potassium chloride |
200.000 |
| Sodium bicarbonate |
1000.000 |
| Sodium chloride |
8000.000 |
| Sodium dihydrogen phosphate anhydrous |
50.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
| Phenol Red |
15.000 |
Quality Control:
- Appearance: Colorless, clear solution
- pH: 7.4
- Osmolality in mOsm/Kg H2O: 270.00 - 310.00
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 4 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
