Tyrode's Balanced Salt Solution With Sodium bicarbonate Without Phenol red: 1X
Cat No.: DCP-TBSSB-R1X
Size: 500 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
[+] Sodium Bicarbonate,
[+] Phenol Red
Description:
Tyrode's Balanced Salts 1X With Sodium Bicarbonate Without Phenol Red is a specialized physiological solution designed for tissue culture and biochemical applications. This ready-to-use liquid formulation provides essential inorganic salts and nutrients to maintain cellular functions in vitro, derived from the original Tyrode's solution developed for mammalian cell metabolism support. Tyrode's uses sodium bicarbonate (1g/L) for CO₂ buffering (pH 7.6-8.2), ideal for incubators with 5% CO₂. Tyrode's Preferred for intestinal muscle studies and calcium-sensitive workflows due to optimized ion ratios.
Applications:
- Mammalian tissue perfusion and maintenance
- Physiological studies using isolated intestinal muscles
- Cellular metabolism research
- Base solution for customized culture media
Advantages:
- Optimized Ion Profile: Contains essential ions (Ca²+, Mg²+, K+, Na+) mimicking extracellular fluid composition, critical for maintaining cellular integrity and muscle function.
- Phenol Red-Free: Eliminates optical interference in fluorescence microscopy, spectrophotometry, and calcium imaging
- Glucose Inclusion: 1g/L provides immediate energy for cell viability during short-term experiments
- Muscle Physiology: Calcium/magnesium content supports smooth muscle contraction studies (e.g., intestinal or cardiovascular tissues)
Composition:
| Ingredients |
mg/L |
| INORGANIC SALTS |
|
| Calcium chloride dihydrate |
265.000 |
| Magnesium chloride anhydrous |
46.900 |
| Potassium chloride |
200.000 |
| Sodium bicarbonate |
1000.000 |
| Sodium chloride |
8000.000 |
| Sodium dihydrogen phosphate anhydrous |
50.000 |
| OTHERS |
|
| D-Glucose |
1000.000 |
Quality Control:
- Appearance: Colorless, clear solution
- pH: 7.4
- Osmolality in mOsm/Kg H2O: 270.00 - 310.00
- Sterility: No bacterial or fungal growth is observed after 14 days of incubation, as per USP specification.
- Toxicity Test: Passes
- Endotoxin Content: NMT 1EU/ml
Storage and Shelf Life:
- Store at 4 °C away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
