Taq Blend with Pfu (200U)

Taq Blend with Pfu (200U)

Cat.No: FNK-02-120

Size: 200 U

Storage: -20℃ 

Concentration: 5 U/µl

Buffer: 35 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50 % glycerol, 0.75 %Tween20, 0.75 % Igepal CA-630 

Quality Assurance: Greater than 95% purity as determined by SDS-PAGE (CBB staining). The absence of endonucleases 3→5amplification was attained with ? DNA template was confirmed. 
 

Product Descripion

Taq Blend with Pfu is optimized blend of Taq and Pfu DNA polymerases. The proof-reading 3’→5’ exonuclease activity of Pfu increases the fidelity and robust amplification of Taq DNA polymerase. The reaction buffer has been formulated for robust yields and long PCR.
 

Component

Taq Blend with Pfu: 5 U/µl Taq and Pfu DNA polymerase    (02-Tbp 40 µl)

5 x Reaction Buffer for Taq Blend with Pfu                        (02-Blb 2 ml)

dNTPs (2.5mM each)                                                             (02-Dnt 640 µl)   

PCR Product

Good amplification result was obtained in PCR reaction using λ phage DNA as a template (Fig.2). 

Data

Experimental Example 

Robustness of Taq Blend with Taq as compared Taq Economy.



Please Note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. NOT FOR MILIARTY USE. 

Related Products

SKU#	Description	Link
FNK-02-120-5	Taq Blend with Pfu (5x200U)	GO!