0.1M Glycine Quenching Buffer with TBS
Catalog No.: DCP-GQTBS0.1X
Size: 500 mL
Non-sterile: Filtered 0.1-micron membrane once, 0.04-micron membrane once
Descriptions:
0.1M Glycine Quenching Buffer with TBS is a ready-to-use, phosphate?free quenching solution formulated to rapidly terminate aldehyde-based crosslinking (e.g., formaldehyde, paraformaldehyde) in fixed cells and tissues. This buffer combines 0.1 M glycine with Tris?Buffered Saline (TBS) to provide efficient quenching while maintaining physiological ionic strength and pH, making it ideal for immunofluorescence, ICC, and phosphoprotein?sensitive applications.
Features:
- TBS base eliminates phosphate interference in phosphoprotein detection and phosphate-sensitive assays.
- Tris-buffered system maintains stable pH across temperature variations, supporting consistent results.
- Glycine's amino group combined with Tris's intrinsic formaldehyde-quenching capacity for synergistic effect.
Applications:
- Phosphoprotein immunofluorescence (IF): Quenching formaldehyde while preserving phospho-epitope integrity.
- Phospho-specific immunocytochemistry (ICC): Preventing phosphate contamination from quenching buffers.
- ChIP-seq (Chromatin Immunoprecipitation): Efficiently terminating formaldehyde crosslinking with enhanced Tris-mediated quenching.
- Phosphokinase and phosphatase studies: Eliminating buffer components that inhibit or interfere with phosphate-dependent assays.
- Multiplex immunofluorescence: Reducing background while preserving multiple epitope recognition.
Composition:
| Ingredients |
Concentration |
| Glycine |
0.1 M |
| Tris base |
10 mM |
| Sodium Chloride |
150 mM |
Recommended Use:
- Fix cells or tissue with formaldehyde or paraformaldehyde according to your protocol.
- Remove fixative and rinse once with TBS or appropriate wash buffer.
- Incubate samples with 0.1M Glycine Quenching Buffer with TBS for 10–30 minutes at room temperature with gentle.
- Proceed to permeabilization, blocking, or antibody staining steps as required.
Quality Control
- Apperance: Clear Solution
- pH: 7.4
- Sterility: Filtered 0.1-micron membrane once, 0.04-micron membrane once in a non-sterile environment
Storage and Shelf Life
- Storage: 4°C (Protect from light and excessive heat)
- Shelf Life: 1 year
* All DiagnoCine Precision Sterile media are filtered-sterilized with a 0.1-micron filtration two times and 0.04 micron once. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
