Uracil-DNA Glycosylase (UDG)

Type:   Molecular Diagnostics,  1000U / 1 vial

Code:  CSB-DEM026


Description:  Uracil-DNA Glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDGefficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases).It releases uracil from ss- or ds-DNA and is applicable to eliminates PCR carry-over contamination.reatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Since UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform.

Application Features :

Treatment of 0.1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA
incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Since UDG remains partially active following heat treatment at 95°C, it is recommended that uracil glycosylase inhibitor be added to prevent degradation of product DNA. Alternatively, reaction products can be immediately extracted with phenol/chloroform.

Molecular Weight:   25 kDa

Storage buffer:   10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.1 mg/ml BSA, 50% Glycerol, pH 7.4

Quality Control:   Uracil-DNA Glycosylase is tested for contaminating activities, with no trace of endonuclease activity, nicking activity or exonuclease

Notes:   UDG is active over a broad pH range with an optimum at pH 8.0, does not require divalent cation, and is inhibited by high ionic strength (> 200 mM).

Shape:   Liquid

Storage:   Long term storage at -20 °C, short term storage at 2-8 °C

Application:   Removal of uracil bases from single or double stranded DNA, Control of carry-over contamination in PCR

Source:   Recombinant in E.coli

Active:   5U/μL

Activity is defined:  One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 µL reaction containing 0.2 µg DNA (104-105 cpm/µg)

Size:   1000 U or user-defined

MOQ price range:
 

Cat#

Product Name

MOQ:The MOQ of different proteins are different. Samples are available. Call now~!

less than 10 vials

10-50 vials

50-100 vials

100-500 vials

500-1000 vials

More than 1000 vials

CSB-DEM026

Uracil-DNA Glycosylase(UDG)

$80

/1000U / 1 vial

$76

/1000U / 1 vial

$68

/1000U / 1 vial

$60

/1000U / 1 vial

$48

/1000U / 1 vial

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