Signal Booster Neo

Product#: BCL-SBN
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Signal Booster Neo 


Cat No: BCL-SBN


Storage: 4°C 

Description


The signal booster series is a group of reagents for enhancing antigen-antibody reaction. We have developed the following two types of products depending on the application. Signal Booster Signal Booster is a protein-containing enhancement reagent that exhibits a very stable enhancement effect. Signal Booster Neo Signal Booster Neo is a protein-free enhancer that can be used for detection of phosphorylated proteins and other detection systems where additional proteins are a problem. All products show enhanced signals in Western blotting, ELISA, etc. simply by diluting the antibody with the product. 

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Principle

The main component of the signal booster series is a polymer and a component that has a blocking action. By changing the physicochemical properties of the antigen and antibody, the polymer increases the frequency of contact between them and enhances the specific antigen-antibody reaction. On the other hand, a blocking component reduces non-specific binding. As a result, the background is lowered and the specific antigen-antibody reaction is enhanced.

Product types 

Signal Booster This is a standard product containing protein components used for Western Blot and ELISA. This product is made up of two liquids, the A liquid for the primary antibody dilution and the B liquid for the secondary antibody. In the case of ELISA, either solution A or solution B shows good results depending on the measurement system.

  1. A signal that is several to several tens of times higher than the conventional method using a buffer containing a surfactant can be obtained
  2. A higher signal can be obtained compared to similar products
  3. Antigen-antibody reaction such as Western and ELISA Can be widely used in various assay systems involved
  4. In addition to detection by HRP and ALP enzyme, it can also be used for color detection and luminescence detection
  5. Easy to use by diluting antibody with this reagent 
 
Product No. Product Name Capacity
BCL100_50 mL Signal Booster Solution A&B set Solution A & B 50mL each
BCL100_100 mL Solution A & B 100mL each
BCL100_250 mL Solution A & B for each 250mL
 
BCL110_A-100 mL Signal Booster Solution 100 mL
BCL110_B-100 mL 100 mL
BCL110_A-250 mL 250 mL
BCL110_B-250 mL 250 mL
 
BCL-SBN_01 Signal Booster Neo 250 mL
BCL-SBN_02 500 mL
 
BCLIS_FMS Signal Booster Immunostain 10 mL each
BCLIS_F Solution F, 20 mL
BCLIS_M Solution M, 20 mL
BCLIS_S Solution S, 20 mL


Features

  • Ready To Use
    • Simply dilute antibodies by Signal Boster.
    • For Primary antibody, dilute by Solution A.
    • For Secondary antibody, dilute by Solution B.
  • Stronger signal and lower background
    • Can get a few to >10 times stronger signals.
  • Stronger signal as compared to similar products
  • Wide variety of applications
    • Can be used for Western Blot, ELISA, etc.
    • HRP or ALP will not be affected by Signal Booster.
    • Luminecense, Fluorescence detection system is also compatible.
"Signal Booster Neo" : Chemically defined, Protein-free signal enhancer.

Other Features
  • "Ready To Use" One solution system
    • Simply dilute Primary antibody and Secondary antibody by Signal Booster Neo.
  • Stronger signal and lower background
    • Can get a few to >10 times stronger signals.
  • For phospho protein detection or for any ELISA
    • Protein-free composition contributes to no interference by proteins in the reagent.
  • Wide variety of applications
    • Can be used for Western Blot, ELISA, etc.
    • HRP or ALP will not be affected by Signal Booster.
    • Luminecense, Fluorescence detection system is also compatible

Example Data
 
(A) Signal Booster is used for detection of Luciferase

NT : TBS (-), SB : Signal Booster, A : Competitor company product
Sample : Luciferaseexpressing HepG2cell lysate
1st Ab : Anti-Luciferase IgG, goat polyclonal, 1/1000 diluted by Solution A
2nd Ab : Anti-Goat IgG, donkey HRP-labeled, 1/20000 diluted by Solution B
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(B) Signal Booster is used for detection of HBsAgS by Sandwich ELISA

Solid phase Ab:Anti-HBsAg Ab, Rabbit polyclonal
Sample : HBsAg-XT (Cat #:BCL-AG-002 )
1st Ab : Anti-HBsAg Ab, Mouse monoclonal ,diluted by Solution A
or Anti-HBsAg Ab, Mouse monoclonal HRP, diluted by Solution B
2nd Ab : Anti-mouse IgG, Goat polyclonal, HRP, diluted by Solution B
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(C) Signal Booster Neo is used for detection of phospho-EGFR

SB : Signal Booster, SBN : Signal Booster Neo
Sample : A431 Cell Lysate
Blocking : c-Block
1st Ab : Anti-pEGFR, Rabbit
2nd Ab : Anti-Rabbit,Goat polyclonal, HRP
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(D) Signal Booster Neo is used for detection of HBs antigen by ELISA

SB : Signal Booster, SBN : Signal Booster Neo
Blocking : b-block
Capture Ab : Anti-HBsAg mouse
Detection Ab : Anti-Mouse IgG-HRP
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(E) Signal Booster Neo is used for detection of mouse serum amyloid A3 by Western Blot.

Sample : Mouse Serum Amyloid A3, overexpressed in E.Coli 1st and 2nd Antibodies are diluted in TBST-dried milk or Signal Booster Neo according to manufacturer’s instructions.
There was a dramatic difference with the Signal Booster Neo.
(Data kindly provided by Dr. Robert Anderberg, Providence Medical Research Center, Providence Health Care)
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