Cat No: FNK-BCL-SBN-01

Storage: 4°C 

Description

The signal booster series is a group of reagents for enhancing antigen-antibody reaction. We have developed the following two types of products depending on the application. Signal Booster Signal Booster is a protein-containing enhancement reagent that exhibits a very stable enhancement effect. Signal Booster Neo Signal Booster Neo is a protein-free enhancer that can be used for detection of phosphorylated proteins and other detection systems where additional proteins are a problem. All products show enhanced signals in Western blotting, ELISA, etc. simply by diluting the antibody with the product. 

 

Product No.	Product Name	Capacity	Link
FNK-BCL-SBN_01	Signal Booster Neo	250 mL
FNK-BCL-SBN_02		500 mL
FNK-BCL-SBN_Trial		Trial

Principle

The main component of the signal booster series is a polymer and a component that has a blocking action. By changing the physicochemical properties of the antigen and antibody, the polymer increases the frequency of contact between them and enhances the specific antigen-antibody reaction. On the other hand, a blocking component reduces non-specific binding. As a result, the background is lowered and the specific antigen-antibody reaction is enhanced.

Product types 

Signal Booster This is a standard product containing protein components used for Western Blot and ELISA. This product is made up of two liquids, the A liquid for the primary antibody dilution and the B liquid for the secondary antibody. In the case of ELISA, either solution A or solution B shows good results depending on the measurement system.

1. A signal that is several to several tens of times higher than the conventional method using a buffer containing a surfactant can be obtained
2. A higher signal can be obtained compared to similar products
3. Antigen-antibody reaction such as Western and ELISA Can be widely used in various assay systems involved
4. In addition to detection by HRP and ALP enzyme, it can also be used for color detection and luminescence detection
5. Easy to use by diluting antibody with this reagent 

Product No.	Product Name	Capacity	Link
FNK-BCL-105	Signal Booster Solution A&B set	Solution A & B 50mL each
FNK-BCL-110		Solution A & B 100mL each
FNK-BCL-125		Solution A & B 250mL each
FNK-BCL-110A	Signal Booster Solution	Solution A, 100 mL
FNK-BCL-110B		Solution B, 100 mL
FNK-BCL-125A		Solution A, 250 mL
FNK-BCL-125B		Solution B, 250 mL
BCLIS_FMS	Signal Booster Immunostain	10 mL each
FNK-BCL-ISF		Solution F, 20 mL
FNK-BCL-ISM		Solution M, 20 mL
FNK-BCL-ISS		Solution S, 20 mL

Features

• Ready To Use
  • Simply dilute antibodies by Signal Boster.
  • For Primary antibody, dilute by Solution A.
  • For Secondary antibody, dilute by Solution B.
• Stronger signal and lower background
  • Can get a few to >10 times stronger signals.
• Stronger signal as compared to similar products
• Wide variety of applications
  • Can be used for Western Blot, ELISA, etc.
  • HRP or ALP will not be affected by Signal Booster.
  • Luminecense, Fluorescence detection system is also compatible.

• "Ready To Use" One solution system
  • Simply dilute Primary antibody and Secondary antibody by Signal Booster Neo.
• Stronger signal and lower background
  • Can get a few to >10 times stronger signals.
• For phospho protein detection or for any ELISA
  • Protein-free composition contributes to no interference by proteins in the reagent.
• Wide variety of applications
  • Can be used for Western Blot, ELISA, etc.
  • HRP or ALP will not be affected by Signal Booster.
  • Luminecense, Fluorescence detection system is also compatible

(A) Signal Booster is used for detection of Luciferase NT : TBS (-), SB : Signal Booster, A : Competitor company product Sample : Luciferaseexpressing HepG2cell lysate 1st Ab : Anti-Luciferase IgG, goat polyclonal, 1/1000 diluted by Solution A 2nd Ab : Anti-Goat IgG, donkey HRP-labeled, 1/20000 diluted by Solution B
(B) Signal Booster is used for detection of HBsAgS by Sandwich ELISA Solid phase Ab:Anti-HBsAg Ab, Rabbit polyclonal Sample : HBsAg-XT (Cat #:BCL-AG-002 ) 1st Ab : Anti-HBsAg Ab, Mouse monoclonal ,diluted by Solution A or Anti-HBsAg Ab, Mouse monoclonal HRP, diluted by Solution B 2nd Ab : Anti-mouse IgG, Goat polyclonal, HRP, diluted by Solution B
(C) Signal Booster Neo is used for detection of phospho-EGFR SB : Signal Booster, SBN : Signal Booster Neo Sample : A431 Cell Lysate Blocking : c-Block 1st Ab : Anti-pEGFR, Rabbit 2nd Ab : Anti-Rabbit,Goat polyclonal, HRP
(D) Signal Booster Neo is used for detection of HBs antigen by ELISA SB : Signal Booster, SBN : Signal Booster Neo Blocking : b-block Capture Ab : Anti-HBsAg mouse Detection Ab : Anti-Mouse IgG-HRP
(E) Signal Booster Neo is used for detection of mouse serum amyloid A3 by Western Blot. Sample : Mouse Serum Amyloid A3, overexpressed in E.Coli 1st and 2nd Antibodies are diluted in TBST-dried milk or Signal Booster Neo according to manufacturer’s instructions. There was a dramatic difference with the Signal Booster Neo. (Data kindly provided by Dr. Robert Anderberg, Providence Medical Research Center, Providence Health Care)