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FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES: 1X Liquid

Product#: DCP-RPMIGH1X
$49.50
DCP-RPMIGH1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640 Medium, High Glucose & 25mM HEPES: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Contains High Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-RPMIGH1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid RPMI 1640 medium engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The train reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Formulated with high glucose (4500 mg/L), L-glutamine (300 mg/L), sodium pyruvate (110 mg/L), and 25 mM HEPES for enhanced pH stability in open-well culture
  • Sodium bicarbonate (2000 mg/L) buffering system maintains physiological pH 7.4 under standard 5% CO₂ incubation — see 2 note below
  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
  • 41 verified ingredients per lot with full CAS traceability across inorganic salts, amino acids, vitamins, and other components
  • Manufactured under an ISO 13485:2016 quality management system with per-lot QC release
  • Ultrapure Type 1 water (18.2 MΩ·cm) used throughout formulation
  • Customizable pH, glucose concentration, HEPES, salts, and nutrient composition available on request — contact support@diagnocine.com
CAT. NO.
DCP-RPMIGH1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
RPMI 1640 Medium, High Glucose & 25mM HEPES: 1X Liquid
  • Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] HEPES (25mM), [+] Phenol Red
  • Glucose4500.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
  • Available Sizes500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and aggregates that can foul microfluidic channels and confound sensitive cell assays.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particulate-controlled media for OoC microfluidic chips.

target

Total metabolic control

Defined high-glucose, glutamine, and pyruvate formulation for precise control of carbon sources and metabolic inputs in your experimental system.

water_drop

Ultrapure-grade water

18.2 MΩ·cm resistivity (ASTM Type I / ISO 3696 equivalent). Trace-metal and organic-carbon (TOC) controlled at the manufacturing source.

visibility

Low background for imaging

Ultra-low particulate baseline for confocal, biosensor, and live-cell imaging applications on-chip.

science

Rich, stable nutrient profile

41 verified ingredients per lot. Full CAS traceability. Micro-batch precision manufacturing.

tune

Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates and debris; protects the first 0.04 µm final filter and extends its service life.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including mycoplasma-sized organisms (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Performance vs. conventional media

FluxMPS™ DCP-RPMIGH1X runs a quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) to a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional sterile filtration — with mycoplasma-retentive (0.1 µm) filtration at every production stage.

5.5×
Finer final pore size than standard 0.22 µm filtration (0.04 µm vs 0.22 µm)
4
Serial filtration passes (0.1 µm ×2 + 0.04 µm ×2)
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma control via 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-RPMIGH1X RPMI 1640 High Glucose 25mM HEPES quadruple-stage filtration system 0.1 micron x2 plus 0.04 micron x2 organ-on-a-chip microfluidic cell culture media Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-RPMIGH1X.
© Diagnocine® — DCP-RPMIGH1X
Applications

OoC and MPS Applications

FluxMPS™ DCP-RPMIGH1X delivers Microfluidics Suitable purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated bioreactors and robotic perfusion systems, adding 0.02 µm and 0.01 µm stages after this product's 0.04 µm polish.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates that foul automated systems
  • Valve & Sensor Protection: Reduces micro-fouling in automated perfusion and recirculation systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Microphysiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip devices where particulate control prevents channel fouling.

OoCToCBoCLoCMPS
Cancer Biology

Cancer Cell Lines & Metabolic Research

Supports NCI-60 cancer lines and Warburg effect metabolic studies with precise glucose and pyruvate control.

MCF-7MDA-MB-231HeLaJurkat
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered base for iPSC differentiation protocols requiring defined, particulate-controlled media.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particulate-controlled formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined formulation for 13C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline for confocal microscopy, biosensor measurements, and TEER monitoring on-chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] HEPES (25mM), [+] Phenol Red, [+] Calcium, [+] Magnesium
Appearance Red-colored, clear solution
Total ingredients 41 (6 inorganic salts, 20 amino acids, 10 vitamins, 5 others)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500.000 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement 5% CO2 required (sodium bicarbonate, 2000 mg/L)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

RPMI 1640 Medium, High Glucose & 25mM HEPES: 1X Liquid: 41 ingredients verified per lot with CAS numbers for full raw-material traceability. All ingredients from the original formulation are preserved exactly.

Component CAS Number mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate 13477-34-4 100.000
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2000.000
Sodium chloride 7647-14-5 6000.00
Sodium phosphate dibasic anhydrous 7558-79-4 800.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 10.000
L-Arginine hydrochloride 1119-34-2 241.000
L-Asparagine 70-47-3 50.000
L-Aspartic acid 56-84-8 20.000
L-Cystine dihydrochloride 30925-07-6 65.200
L-Glutamic acid 56-86-0 20.000
L-Glutamine 56-85-9 300.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.960
L-Hydroxyproline 51-35-4 20.000
L-Isoleucine 73-32-5 50.000
L-Leucine 61-90-5 50.000
L-Lysine hydrochloride 657-27-2 40.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 15.000
L-Proline 147-85-3 20.000
L-Serine 56-45-1 30.000
L-Threonine 72-19-5 20.000
L-Tryptophan 73-22-3 5.000
L-Tyrosine Disodium Salt 69847-45-6 28.830
L-Valine 72-18-4 20.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 3.000
D-Biotin 58-85-5 0.200
D-Ca-Pantothenate 137-08-6 0.250
Folic acid 59-30-3 1.000
Niacinamide 98-92-0 1.000
Pyridoxine hydrochloride 58-56-0 1.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 35.000
p-Amino benzoic acid (PABA) 150-13-0 1.000
OTHERS
D-Glucose 50-99-7 4500.000
Glutathione reduced 70-18-8 1.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 5.300
Sodium Pyruvate 113-24-6 110.000
Customization available: pH, glucose, HEPES, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-RPMIGH1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity, ASTM Type I / ISO 3696 equivalent. Trace-metal and organic-carbon (TOC) controlled at the manufacturing source.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in ISO Class 5 (Class 100) cleanroom. Immediate post-filtration fill to reduce recontamination risk.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Every batch tested independently — not pooled or blended across lots.

Endotoxin USP <85> BET

LAL-based endotoxin test; assay sensitivity 0.005 EU/mL. Batch release specification: < 0.05 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate count test. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>.

Documentation / CoA

Full Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-RPMIGH1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter DCP-RPMIGH1X (FluxMPS™) Conventional 0.22 µm Filtered Standard Alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] HEPES Standard RPMI 1640 Standard RPMI 1640
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle cancel cancel
Water quality Type 1, 18.2 MΩ·cm Type 2 typical Type 2 typical
Manufacturing QMS ISO 13485:2016 ISO 9001 typical Variable
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMIGH1X and its use in OoC and MPS applications.

Yes. DCP-RPMIGH1X is a Microfluidics Suitable medium engineered for MPS and OoC platforms. The 0.04 µm final filtration helps prevent microfluidic channel fouling, making it suitable for OoC, ToC, BoC, and LoC applications sensitive to particulate contamination.
FluxMPS uses a quadruple-stage system (0.1 µm ×2 + 0.04 µm ×2), reaching a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used in conventional sterile filtration — with mycoplasma-retentive (0.1 µm) filtration that standard 0.22 µm processing does not include.
This formulation includes high glucose (4500 mg/L), L-glutamine (300 mg/L), sodium pyruvate (110 mg/L), sodium bicarbonate (2000 mg/L), and 25 mM HEPES for enhanced pH stability during open-well handling. It is provided as a basal medium; supplement with serum, growth factors, or other additives per your protocol.
Yes. The sodium bicarbonate (2000 mg/L) buffering system is formulated to maintain physiological pH 7.4 under standard 5% CO2 incubation. Incubate at 37°C with 5% CO2 unless your protocol specifies otherwise.
Yes. This is a basal medium formulation. Supplement with FBS, serum replacements (e.g., B27, N2), or growth factors as required. For serum or protein-containing additions, use a 0.2 µm low-protein-binding PES or PVDF syringe filter; 0.1 µm is appropriate only for defined, protein-free additions. Do not use a 0.04 µm filter for supplements, as it will strip serum of essential lipoproteins and growth factors.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, verified by a Limulus Amebocyte Lysate (LAL) assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) before each batch is released.
Yes. CoA is available per lot upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin, sterility, USP <788> particulate data, appearance, and full raw material lot traceability.
Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
  4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
  5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
  6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
  7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
  8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
  9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
  10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

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