Plant RNA Mega Kit (2 Preps)
Cat. No.: BMG-R6615-01
Size: 2 Preps
Description:
The Plant RNA Mega Kit is designed for the extraction and purification of RNA from large quantities of plant material.
Applications:
- The high-quality RNA extracted using the kit can be used in quantitative real-time PCR (qRT-PCR) and reverse transcription PCR (RT-PCR) to study gene expression levels in plants.
- The kit is suitable for preparing RNA for RNA sequencing, which allows for comprehensive analysis of the transcriptome and identification of gene expression patterns.
- The RNA can be used to construct cDNA libraries, which are essential for cloning and functional studies of plant genes.
- The kit provides RNA suitable for Northern blot analysis, a technique used to detect and quantify specific RNA molecules within a sample.
- Purified RNA can be used in microarray experiments to study the expression of thousands of genes simultaneously, providing insights into gene regulation and interaction.
Features:
- The kit is capable of purifying total RNA from up to 10 grams of plant samples, making it suitable for large-scale RNA extractions.
- These columns are used to bind RNA efficiently during the purification process, ensuring high-quality RNA extraction.
- The kit includes a range of buffers such as Buffer PL, Buffer P2, Buffer LY, and RNA Wash Buffer, which are essential for the various stages of RNA extraction and purification.
- An optional DNase Stop Buffer is provided to remove any residual DNA, ensuring the purity of the RNA.
- The inclusion of DEPC-treated water helps maintain RNA integrity by preventing RNase activity during the extraction process.
References:
Morán, F., Canales, C., Olmos, A., & Ruiz-García, A. B. (2021). First report of grapevine asteroid mosaic associated virus in grapevine in Spain. Plant Disease, 105(2), 517. https://doi.org/10.1094/pdis-07-20-1566-pdn
