Soil gDNA Kit (4 Preps)
Cat. No.: BMG-GD2412-00
Size: 4 Preps
Description:
A Soil gDNA Kit is a specialized tool designed for extracting high-quality genomic DNA from soil samples.
Applications:
- PCR and qPCR for detecting and quantifying specific microorganisms or genes.
- Next-generation sequencing for metagenomic analysis and community profiling.
- Restriction enzyme digestion for genetic fingerprinting and cloning.
- Environmental monitoring to assess soil health and detect contaminants.
- Agricultural research to study plant-microbe interactions and soil microbial communities.
- Ecological studies to investigate microbial diversity and succession in different ecosystems.
- Rapid extraction process (30-40 minutes).
- High DNA yield (up to 5 μg from 250-500 mg soil).
- These kits can extract DNA from various soil types, including clay, sandy, peaty, chalky, and loamy soils.
- Effective removal of PCR inhibitors.
- Kits can isolate DNA from diverse microorganisms including bacteria (Gram-positive and Gram-negative), archaea, fungi, yeast, and algae.
- Easy-to-use components (e.g., pre-filled bead beating tubes).
- Silica membrane technology for DNA purification.
- No need for organic extractions.
- The purified DNA is suitable for PCR, restriction enzyme digestion, Southern blotting, and next-generation sequencing.
- Lysis buffers
- Inhibitor removal buffers
- Binding buffers
- Wash buffers
- Elution buffers
- Spin columns
- Collection tubes
- Bead beating tubes with ceramic or glass beads
Devi SG, Fathima AA, Radha S, Arunraj R, Curtis WR, Ramya M. A Rapid and Economical Method for Efficient DNA Extraction from Diverse Soils Suitable for Metagenomic Applications. PLoS One. 2015 Jul 13;10(7):e0132441. doi: 10.1371/journal.pone.0132441. PMID: 26167854; PMCID: PMC4500551.
Yeates C, Gillings MR, Davison AD, Altavilla N, Veal DA. Methods for microbial DNA extraction from soil for PCR amplification. Biol Proced Online. 1998 May 14;1:40-47. doi: 10.1251/bpo6. PMID: 12734590; PMCID: PMC140122.
Yeates C, Gillings MR, Davison AD, Altavilla N, Veal DA. Methods for microbial DNA extraction from soil for PCR amplification. Biol Proced Online. 1998 May 14;1:40-47. doi: 10.1251/bpo6. PMID: 12734590; PMCID: PMC140122.
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