Bacterial DNA Kit (50 Preps)

Product#: BMG-GD2411-01
$110.00
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Bacterial DNA Kit (50 Preps)

Cat. No.: BMG-GD2411-01
Size: 50 Preps
 

Description:

A Bacterial DNA Kit is a specialized tool designed for the extraction and purification of genomic DNA from bacterial cells. These kits are essential for various molecular biology applications and research involving bacterial genetics.

Research Applications: 
  • Bacterial DNA kits are essential for isolating genomic DNA to study bacterial genetics, gene function, and molecular mechanisms.
  • These kits enable extraction of bacterial DNA from complex environmental or clinical samples for microbiome sequencing and analysis.
  • Extracted DNA can be used for bacterial strain identification and differentiation through techniques like PCR and sequencing.
Diagnostics Applications:
  • Bacterial DNA kits allow isolation of DNA from clinical samples to detect and identify bacterial pathogens using PCR or sequencing methods.
  • Extracted DNA can be analyzed to detect antibiotic resistance genes and guide treatment decisions.
  • These kits enable detection of fastidious or unculturable bacteria directly from clinical specimens.
Other Applications: 
  • Bacterial DNA kits are used to extract DNA from environmental samples like soil, water, or air for microbial community analysis.
  • They allow detection of foodborne pathogens in food products and production environments.
  • Bacterial DNA extraction is useful in forensic microbiology applications.
Extraction Methods: 
  • Spin column-based methods using silica membranes.
  • Magnetic bead-based systems for automated processing.
  • Filter plate formats for high-throughput applications.
Components:
  • Lysis buffers to break open bacterial cell walls.
  • Enzymes like lysozyme and proteinase K for protein digestion.
  • Binding buffers to facilitate DNA adsorption to silica membranes or magnetic beads.
  • Wash buffers to remove impurities.
  • Elution buffer to recover purified DNA.
Features:
  • Rapid extraction from both Gram-positive and Gram-negative bacteria.
  • High DNA yield (up to 30 μg) with sizes ranging from 200 bp to 50 kb.
  • Safe, phenol-free protocols using spin columns or magnetic beads.
  • Complete removal of PCR inhibitors.
  • Processing capacity of up to 1 x 10^9 bacterial cells per preparation.
  • Short processing time (30-60 minutes for multiple samples).
Reference:
Smith K, Diggle MA, Clarke SC. Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples. J Clin Microbiol. 2003 Jun;41(6):2440-3. doi: 10.1128/JCM.41.6.2440-2443.2003. PMID: 12791861; PMCID: PMC156519.

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