Plant DNA Mega Kit (1 Preps)
Cat. No.: BMG-GD2615-00
Size: 1 Preps
Description:
The Plant DNA Mega Kit is designed for large-scale purification of DNA from plant tissues.
Applications:
- The DNA extracted using the Plant DNA Mega Kit is suitable for PCR, enabling amplification of specific DNA sequences for various genetic analyses.
- The purified DNA can be used in restriction digestion, which involves cutting DNA into smaller fragments using specific enzymes, a crucial step in cloning and mapping studies.
- The DNA is suitable for hybridization techniques, such as Southern blotting, which are used to detect specific DNA sequences within a complex mixture.
Features:
- The kit can process a substantial amount of plant tissue, typically up to 2-5 grams, making it suitable for large-scale DNA extractions.
- It is optimized for the recovery of genomic DNA up to 60 kb in size from both fresh and dried plant tissue samples.
- The kit utilizes spin column technology, which combines the reversible nucleic acid-binding properties of the matrix with speed and versatility. This helps eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates.
- The system is designed to provide high-quality DNA suitable for various applications, including PCR, restriction digestion, and hybridization techniques.
Reference:
Yin, Y., Liu, X., Wang, B., Gao, L., & Zhang, X. (2017). [DNA molecular identification of Manis pentadactyla]. PubMed, 42(11), 2078–2084. https://doi.org/10.19540/j.cnki.cjcmm.2017.0092
Yu, S., Wu, Y., & Song, W. (2022). Occurrence of a 16SrII-V Subgroup Phytoplasma Associated with Witches’-Broom Disease in Melochia corchorifolia in China. Plant Disease, 106(2), 754. https://doi.org/10.1094/pdis-07-21-1375-pdn
