Key to the kit is our proprietary Buffer RET system that allows the high efficient binding of DNA to our ezBindTM matrix while endotoxin, proteins and other contaminates are removed under certain optimal conditions. Nucleic acids are easily eluted with sterile water or TE buffer. The purified DNA is ion exchange resin residues free which enable the high performance of downstream applications such as transfection, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.
Storage and Stability
Buffer A1 should be stored at 4°C once RNase A is added. All other materials can be stored at room temperature. The Guaranteed shelf life is 18 months from the date of purchase.
Kit Contents
Catalog#
BMG-PD2468-01
BMG-PD2468-2
Preps
10
25
Midi Columns
10
25
Syringe Filters
10
25
Buffer A1
60 mL
130 mL
Buffer B1
60 mL
130 mL
Buffer N3
35 mL
80 mL
Buffer RET*
120 mL
150 mL
Elution Buffer
15 mL
30 mL
DNA Wash Buffer**
50 mL
2x50 mL
RNase A
200 µL
450 µL
Collection Tubes
20
50
Wrench
1
1
*Buffer RET and N3 contains acetic acid, wear gloves and protective eyewear when handling.
**Add 200 mL 100% ethanol to each DNA Wash Buffer before use.
Important:
RNase A: Spin down RNase A vial briefly. Add the RNase A solution to buffer A1 and mix well before use.
Buffer B1 precipitates below room temperature, it is critical to warm up the buffer at 37°C to dissolve the precipitates before use.
Keep the cap tightly closed for buffer B1 after use.
Ensure the availability of centrifuge capable of 13,000 rpm.
Carry out all centrifugations at room temperature.
Materials required but not supplied
100% ethanol
Vacuum manifold if use manifold method.
1.5 mL Eppendorf tubes.
Important Notes
The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids
