Can Get Signal ® immunostain

Product#: NKB-401
$109.00
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Can Get Signal ® immunostain

Cat.No. NKB-401
Store at 4ºC

DESCRIPTION

Can Get Signal® immunostain is a reaction solution that contains an accelerator for antigen-antibody reactions, which improves sensitivity, specificity, and S/N of immunohistochemistry (IHC) and immunocytochemistry.

FEATURES
  • Improves sensitivity, specificity, and S/N of IHC.
  • Can be applied to various detection systems (e.g., chromogenic, chemiluminescence, or fluorescence).
  • Can be used with ABC or polymer complex methods.
  • Solutions A and B exhibit various properties for improving results.
  • Can be used directly without dilution (Ready-to-use).
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APPLICATIONS
  • Immunohistochemistry (IHC)
  • Immunocytochemistry
COMPONENTS
This kit includes the following components. All reagents should be stored at 4ºC and protected from light.
 

Reagent Name

Code No.

NKB-401

NKB-501

NKB-601

Solution A

5 ml

20 ml

-

Solution B

5 ml

-

20 ml


 
TYPICAL REACTION FLOW
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Flow chart of immunostaining with Can Get Signal® immunostain

APPLICATION DATA
 
Example 1. Detection of PCNA using paraffin-embedded sections
The localization of PCNA (proliferating cell nuclear antigen) expression in human skin was detected using paraffin-embedded sections of the human skin tissue model TESTSKINTM (Toyobo). Detection was performed by the ABC method with anti-PCNA mouse monoclonal antibody as the primary antibody and biotinylated mouse IgG as the secondary antibody. Each antibody was diluted with Solution A of Can Get Signal® immunostain prior to use. As a control experiment, PBS(-) containing 1.5% normal horse serum (conventional method) was used instead of Solution A of Can Get Signal® immunostain. As a result, Can Get Signal® immunostain produced higher signals and lower background than the conventional method.
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Example 2. Detection of paxillin with the fluorescent antibody method
The localization of paxillin in Swiss 3T3 cells was detected using anti-paxillin polyclonal antibody and Alexa488-conjugated rabbit IgG antibody. As a result, the exposure time for detection could be reduced from 3 s to 1 s by using Can Get Signal immunostain®, and the detailed fiber structure (fibrillar adhesion) could also be detected by using Can Get Signal® immunostain. However, the structure could not be detected with the conventional method due to high background signals.
Fig. 2C shows a merged picture of the immunological detection with anti-paxillin and anti-phospho-tyrosine antibodies, and actin staining.
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Fig. 2. Immunocytochemistry detection of paxillin with the fluorescent antibody method
*The data was kindly provided by Dr. Harada, Tokyo Institute of Technology.
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