Mannitol Salt Agar Plates/W Tetracycline
Catalog.No: DCP-MSAPT
Features:
Uses: Selective & presumptive identification of Staphlyococcus aureus from clinical, food, cosmetic, and environmental samples
pH: 7.4 ± 0.2
Size: 100 mm
Concentration: 20µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: red to pink-red, clear to slightly opalescent
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Mannitol Salt Agar (MSA) with tetracycline is a modified MSA in which tetracycline antibiotic is added to provide an extra layer of selection on top of the high-salt and mannitol fermentation system. MSA contains peptones and beef extract which supply nitrogen, amino acids, vitamins, minerals, and other growth factors required for bacterial growth. The NaCl inhibits most non-halotolerant bacteria and enriches for salt-tolerant Gram-positive organisms. D-mannitol is the fermentable carbohydrate and the phenol red acts as a pH indicator. The medium is red at neutral to alkaline pH and becomes yellow where mannitol fermentation produces acid.
Tetracycline is a broad-spectrum bacteriostatic antibiotic that binds to the 30S ribosomal subunit and blocks attachment of aminoacyl-tRNA, thereby inhibiting protein synthesis in susceptible bacteria. When tetracycline is added to MSA, it introduces an extra selection layer. Bacteria that can grow at the high salt concentration and are resistant to tetracycline will grow, so the medium remains selective for salt-tolerant organisms and differential for mannitol fermentation via mannitol and phenol red.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
