DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
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Cat Glucagon Like Peptide 1 (GLP-1)
Cat.No.: JNB-JL54755
Size: 96TSpecies: CatAssay Type: CompetitiveSensitivity: 35.25pg/mLDetection Range: 78.12-5000pg/mLAlternative Names: Glucagon Like Peptide 1Research Areas: Cardiovascular BiologyAssay Time: 1h45mAbbreviation: GLP-1Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with Cat GLP-1 antigen. Add Sample, Standard, Biotin-antibody, and Streptavidin-HRP by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is opposite to the Cat GLP-1 in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Cat GLP-1 in the samples by comparing the OD of the samples to the standard curve.Specificity: This kit recognizes Cat GLP-1 in samples. No significant cross-reactivity or interference between Cat GLP-1 and analogues was observed.Recovery rate: 80%-120%Precision: Intra-CV and Inter-CV <10% Shipping: Ice Bag