Mannitol Salt Agar Plates/W Kanamycin
Catalog.No: DCP-MSAPK
Features:
Uses: Selective & presumptive identification of Staphlyococcus aureus from clinical, food, cosmetic, and environmental samples
pH: 7.4 ± 0.2
Size: 100 mm
Concentration: 50µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: red to pink-red, clear to slightly opalescent
Sterile
Shelf life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Mannitol salt agar (MSA) with kanamycin is a modified version of standard MSA in which an aminoglycoside antibiotic is added to increase suppression of kanamycin-susceptible background bacteria, while preserving the medium's normal salt selectivity and mannitol-based differentiation for staphylococci. MSA contains peptones and beef extract, which provides nitrogen, amino acids, vitamins, minerals, and other growth factors needed for bacterial growth. The NaCl included has a high-salt level that inhibits most non-halotolerant organisms and enriches for salt-tolerant Gram-positive bacteria such as staphylococci and some micrococci. D-mannitol is the fermentable carbohydrate, and phenol red is the pH indicator. The medium is red at neutral/alkaline pH and turns yellow around colonies that ferment mannitol and produce acid.
Kanamycin is an aminoglycoside antibiotic that binds to the 30S ribosomal subunit and interferes with translation causing a misreading of mRNA and inhibition of protein synthesis in susceptible bacteria. It is active against many aerobic Gram-negative rods and some Gram-positive organisms. The addition of kanamycin to MSA provides an extra layer of selection in addition to the high-salt barrier, so only bacteria that can tolerate the salt concentration and are resistant to kanamycin will grow well.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
