FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Formulated without L-glutamine and sodium pyruvate, with D-galactose supplied as the defined carbon source for CO₂-independent culture. Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× fewer particles ≥10 µm than conventional 0.22 µm-filtered media.

• Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2) — mycoplasma-retentive at every pass
• Formulated without L-glutamine and sodium pyruvate; 0.9 g/L D-galactose supplied as the defined carbon source
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>)
• CO₂-independent formulation — no sodium bicarbonate; suitable for open-well or sealed microfluidic culture without a CO₂ incubator
• Manufactured under an ISO 13485:2016 quality management system; final QC and finishing at Diagnocine, Totowa, NJ
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) and filled in an ISO Class 5 (Class 100) environment
• Contains phenol red (11 mg/L) as a pH indicator — plan accordingly for fluorescence/absorbance-sensitive assays
• Custom pH, galactose concentration, HEPES, salts and nutrient levels available on request — support@diagnocine.com

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

Quadruple-stage filtration system

This 1X liquid medium is processed through a validated four-stage sequential filtration reaching a final pore size of 0.04 µm — capturing particulates and mycoplasma-scale bioburden that 0.22 µm filtration passes through.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates, including mycoplasma-scale bioburden (0.2–0.3 µm), that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   A second, dedicated prefilter protecting the second 0.04 µm final-filter cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill and finish in an ISO Class 5 (Class 100) laminar-flow environment.

Optimized for next-generation cell biology platforms

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

For automated perfusion bioreactors and robotic liquid-handling platforms, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, built on a six-stage cascade beyond the 0.04 µm Microfluidics Suitable line described on this page.

• Total particulate exclusion beyond the 0.04 µm Microfluidics Suitable tier
• Valve and sensor protection for long-duration automated perfusion runs
• Extended perfusion stability with reduced fouling risk on sensitive flow paths

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request it.

Full technical specification

Every lot of FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid is released against multi-parameter QC specifications.

Available sizes: 500 mL, 1000 mL.

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 33 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

How DCP-LL15-QP1X compares

Filtration depth, mycoplasma barrier, water quality, and QC depth compared with conventional 0.22 µm filtered L-15 media.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 1X Liquid (DCP-LL15-QP1X).

Supporting literature

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180.
4. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
5. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
6. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
7. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
8. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7