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FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth(TPB): 1X Liquid

Product#: DCP-GMEM-N1X
$49.50
DCP-GMEM-N1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth(TPB): 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose

FluxMPS™ DCP-GMEM-N1X is a Microfluidics Suitable, Quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid GMEM formulation with High Glucose and Tryptose Phosphate Broth (TPB), engineered for organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Sterile Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) validated for microfluidic-safe purity
  • High-glucose GMEM base (4500 mg/L D-glucose) enriched with Tryptose Phosphate Broth (2950 mg/L)
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Sodium bicarbonate–buffered (2750 mg/L NaHCO3) formulation; elevated CO₂ required to maintain pH 7.4 (see Specifications)
  • Manufactured under an ISO 13485:2016 quality management system with full lot traceability
  • Phenol red indicator included (15 mg/L) for visual pH monitoring
  • pH, glucose concentration, salts, and nutrient composition available on request
CAT. NO.
DCP-GMEM-N1X | Cell culture media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth: 1X Liquid
  • Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium
  • Glucose4500.000 mg/L
  • L-Glutamine292.000 mg/L
  • Tryptose Phosphate Broth2950.000 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that confound sensitive on-chip assays.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 (light obscuration) particulate compliance. Formulated to reduce particulate load for OoC microfluidic chips.

target

Total metabolic control

High-glucose, bicarbonate-buffered GMEM base enriched with Tryptose Phosphate Broth for defined control of carbon source and nutrient inputs in your experimental system.

water_drop

Ultrapure-grade water

Manufactured with Type 1 water (18.2 MΩ·cm) for tightly controlled trace-metal and organic-carbon (TOC) content at the point of manufacture.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and biosensor applications on-chip. (Note: phenol red is present in this formulation — see Composition & Specifications for optical considerations.)

science

Rich, stable nutrient profile

31 verified ingredients per lot, including Tryptose Phosphate Broth. Full CAS traceability where established. Micro-batch precision manufacturing.

tune

Customization on demand

pH, glucose concentration, salts, and nutrient composition modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages, run as two dedicated prefilter-plus-final-filter pairs, reaching a final 0.04 µm polish — engineered for microfluidic-safe purity in organ-on-a-chip and MPS applications.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge and extends its service life.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; provides a mycoplasma-retentive barrier (validated pore size versus organisms 0.2–0.3 µm in diameter). Filtration is a risk-mitigation step, not a substitute for mycoplasma testing.

  3. 3

    0.1 µm Prefiltration II

    Second, independent prefilter dedicated to protecting the second 0.04 µm final filter cartridge in the train.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter ahead of aseptic fill & finish (ISO Class 5 / Class 100). Final product QC release gate.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 validated filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is by 0.1 µm / 0.04 µm mycoplasma-retentive filtration applied at every lot; this is a filtration statement, not a per-lot mycoplasma test result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-GMEM-N1X Glasgow Minimum Essential Medium GMEM High Glucose Tryptose Phosphate Broth Quadruple-stage filtration organ-on-a-chip microfluidic cell culture media Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) — DCP-GMEM-N1X.
© Diagnocine® — DCP-GMEM-N1X
Applications

OoC and MPS Applications

FluxMPS™ DCP-GMEM-N1X delivers Microfluidics Suitable, ultra-filtered purity for organ-on-a-chip and microfluidic applications requiring a high-glucose GMEM base with Tryptose Phosphate Broth.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactors and robotic perfusion systems. This is a distinct, higher tier than the Microfluidics Suitable (0.04 µm) product described on this page.

  • Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates that can foul automated systems
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion and recirculation systems
  • Extended Perfusion Stability: Supports consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

0.04 µm filtered media for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip platforms where reduced particulate load helps prevent channel fouling.

OoCToCBoCLoCMPS
Cancer Biology

Cancer Cell Lines & Metabolic Research

High-glucose formulation supports NCI-60 cancer cell lines and Warburg-effect metabolic studies with a defined nutrient background.

MCF-7MDA-MB-231HeLaJurkat
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered base for iPSC differentiation protocols requiring reduced particulate background.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Reduced-particulate formulation for primary cells and endothelial monolayer studies on-chip.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined, high-glucose formulation supports ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains both sodium bicarbonate and phenol red.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Reduced particulate background supports confocal microscopy and biosensor measurements on-chip; note phenol red presence when planning absorbance/fluorescence-based assays.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available pack sizes: 500 mL, 1000 mL. Certificate of Analysis available: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium
Appearance Red to reddish-orange, clear solution (phenol red indicator, 15 mg/L)
Total ingredients 31
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 4500.000 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm / 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement Approximately 7% CO₂ (bicarbonate-buffered, 2750 mg/L NaHCO3, calculated to maintain pH 7.4); validate empirically for your incubator
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth: 1X Liquid — 31 ingredients verified per lot with CAS numbers for raw-material traceability where established. All ingredients from the original formulation are preserved exactly.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulphate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 2750.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 42.000
L-Cystine 30925-07-6 24.000
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride 5934-29-2 21.000
L-Isoleucine 73-32-5 52.400
L-Leucine 61-90-5 52.400
L-Lysine hydrochloride 657-27-2 73.100
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 33.000
L-Threonine 72-19-5 47.600
L-Tryptophan 73-22-3 8.000
L-Tyrosine Disodium salt 69847-45-6 52.000
L-Valine 72-18-4 46.800
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 2.000
D-Ca-Pantothenate 137-08-6 2.000
Folic acid 59-30-3 2.000
Nicotinamide 98-92-0 2.000
Pyridoxal hydrochloride 65-22-5 2.000
Riboflavin 83-88-5 0.200
Thiamine hydrochloride 67-03-8 2.000
i-Inositol 87-89-8 3.600
OTHERS
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.000
Tryptose Phosphate Broth   2950.000
Customization available: pH, glucose concentration, salts, and nutrient composition modifications available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485 Manufacturing & Compliance

Every batch of FluxMPS™ DCP-GMEM-N1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

verified

ISO 13485:2016 QMS

Full quality management system with documented procedures, deviation control, and CAPA. Every lot traceable from raw material to final release.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity, controlled for trace metals and organic carbon at the point of manufacture.

biotech

ISO Class 5 Fill & Finish

Final aseptic filling in ISO Class 5 (Class 100) cleanroom. Immediate post-filtration fill to reduce recontamination risk.

assignment

Micro-Batch Precision

Small-batch manufacturing with per-lot QC release. Every batch tested independently — not pooled or blended across lots.

Endotoxin USP <85> BET

Endotoxin is controlled per manufacturing batch, not per unit. LAL assay per USP <85>; assay sensitivity 0.005 EU/mL; batch release specification < 0.05 EU/mL.

Particulate USP <788> Method 1

Light obscuration particulate analysis. NMT 25/mL at ≥10 µm; NMT 3/mL at ≥25 µm.

Osmolality USP <785>

Osmolality verified per lot using vapor pressure or freezing-point depression osmometry per USP <785>. Contact for specification.

Documentation / CoA

Full Certificate of Analysis available per lot. Includes all QC parameters, test dates, and raw material lot numbers. Request: support@diagnocine.com.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-GMEM-N1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Parameter DCP-GMEM-N1X (FluxMPS™) Conventional 0.22 µm Filtered Standard Alternative
Grade Microfluidics Suitable Not specified Not specified
Formulation [+] High Glucose, [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] Calcium, [+] Magnesium Standard GMEM Standard GMEM
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle cancel cancel
Water quality Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEM-N1X and its use in OoC and MPS applications.

Yes. DCP-GMEM-N1X is a Microfluidics Suitable medium engineered for MPS and OoC platforms. The 0.04 µm final filtration is intended to reduce particulate load that can foul microfluidic channels, making it suitable for OoC, ToC, BoC, and LoC applications.
FluxMPS uses a Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2, run as two prefilter-plus-final-filter pairs), delivering approximately 5× lower particulate counts and a mycoplasma-retentive final pore size that standard 0.22 µm filtration does not provide.
Tryptose Phosphate Broth (2950 mg/L) is included as a rich source of amino acids, peptides, and growth-supporting factors, complementing the high-glucose (4500 mg/L) GMEM base and 292 mg/L L-glutamine already present. Use this formulation as supplied; do not add additional TPB, glucose, or glutamine unless your protocol specifically requires elevated concentrations beyond what this formulation already provides.
Yes. This formulation is sodium bicarbonate–buffered (2750 mg/L NaHCO3). Based on the Henderson-Hasselbalch relationship, maintaining the target pH of 7.4 requires approximately 7% CO₂ in a humidified incubator at 37°C; validate empirically for your specific incubator and application.
Yes. This is a basal medium formulation. Supplement with FBS, serum replacements, or growth factors as required by your cell type and protocol. When filtering serum-containing or protein-containing supplements prior to addition, use a 0.2 µm low-protein-binding PES or PVDF filter — do not use a 0.04 µm filter for serum or protein-containing additions, as it will retain much of the protein and lipoprotein fraction.
The batch release specification is < 0.05 EU/mL, verified by the Limulus Amebocyte Lysate (LAL) Bacterial Endotoxin Test (BET) per USP <85> for every manufacturing batch (assay sensitivity 0.005 EU/mL). This is a per-batch release specification, not a per-unit certificate.
Yes. A CoA is available per batch upon request at support@diagnocine.com. It includes pH, osmolality, endotoxin, sterility, mycoplasma-retentive filtration status, USP <788> particulate data, appearance, and raw material lot traceability.
Scientific References

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media.

  1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
  2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
  4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
  5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
  6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
  7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
  8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
  9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
  10. Warburg O. On the origin of cancer cells. Science. 1956;123(3191):309–314.doi:10.1126/science.123.3191.309

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