FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 2X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ Leibovitz's L-15

FluxMPS™ DCP-LL15-QP2X is an MPS-grade, ultra-filtered Leibovitz's L-15 2X formulation engineered for Primary cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. CO₂-free by design — uses D-galactose and high amino acid buffering for atmospheric pH maintenance. Formulation: [+] 1.8 g/L D-Galactose | [-] L-Glutamine, [-] Sodium Pyruvate.

CO₂-free by design: D-galactose energy source + high amino acid buffering — no gas supplementation required
0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill

CAT. NO.

DCP-LL15-QP2X | UNSPSC 41122100 Cell Culture Media

Leibovitz's L-15 — 1X Liquid

Media familyLeibovitz's L-15 2X
Carbon (Galactose)1800 mg/L (1.8 g/L, D-Galactose)
Formulation[+] 1.8 g/L D-Galactose | [-] L-Glutamine, [-] Sodium Pyruvate
AppearanceOrange-Red colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)620.00–680.00 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Shelf Life12 months / 2–8°C

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's passes mycoplasma, subvisible particulates, and endotoxin fragments that interfere with CO₂-free pH maintenance and live imaging clarity. FluxMPS™ eliminates these failure modes.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-free L-15 for clear live imaging and CO2-free chip loading.

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CO₂-free by design formulation

Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.

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Ultrapure-grade water

18.2 MΩ·cm — eliminates ionic contaminants that interfere with L-15 amino acid buffering capacity.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL — prevents LPS-driven inflammatory activation in primary cells.

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CO₂-free design

D-galactose + high amino acid buffering maintains pH 7.2–7.5 in atmospheric conditions — no CO₂ required.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Pre-filtration I — Large Particulate Removal

Removes large aggregates; protects downstream 0.04 µm membranes.
2

0.04 µm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
3

0.1 µm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 µm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-LL15-QP2X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered Leibovitz's, with confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.

FluxMPS-Quadruple-stage-filtration-system-Cell-Culture-Media-Diagnocine-V2.jpg — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-LL15-QP2X

Applications

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15-QP2X — Leibovitz's L-15 2X — delivers 0.04 µm filtered purity for Primary cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Live-Cell Imaging

Open-Stage & CO₂-Free Microscopy

L-15 + galactose maintains pH without CO₂ — the standard base for prolonged live-cell confocal, TIRF, light-sheet, and spinning disk imaging on open-stage microscopes.

ConfocalTIRFLight-sheetOpen-stage

Cell Biology

Primary Cell Dissociation & Transport

CO₂-free L-15 is the standard dissociation and transport buffer for primary cells, tissue pieces, and organoids outside the incubator.

Primary cellsOrganoidsTissue dissociation

Microfluidics

OoC Loading & Priming

CO₂-independent L-15 enables chip loading, priming, and cell seeding outside incubators without pH drift during the critical chip assembly steps.

OoC loadingChip primingCell seeding

In Vivo Studies

In Vivo Imaging & Intravital Microscopy

L-15 is the standard superfusion medium for intravital microscopy, maintaining pH in open tissue preparations without CO₂ during surgical and imaging procedures.

IntravitalSuperfusionIn vivo

Metabolomics

CO₂-Free Metabolic Studies

Galactose-based energy source enables metabolic studies without CO₂ interference. Galactose forces OXPHOS in metabolically flexible cells.

OXPHOSGalactose forcingMetabolomics

Neuroscience

Acute Brain Slice & Neuron Imaging

CO₂-free L-15 maintains neuronal viability in acute brain slice preparations and dissociated neuron imaging without requiring carbogen (95% O₂/5% CO₂) gassing.

Brain slicesPrimary neuronsNeuronal imaging

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] 1.8 g/L D-Galactose \| [-] L-Glutamine, [-] Sodium Pyruvate
Appearance	Orange-Red colored, clear solution
Carbon source	D-Galactose: 1800 mg/L (1.8 g/L, D-Galactose)
pH USP <791>	7.4
Osmolality USP <785>	620.00–680.00 mOsm/kg H₂O
Total ingredients	33

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	cold pack
CO₂ requirement	CO₂-independent by design — Leibovitz's L-15 uses galactose and high amino acid buffering for CO₂-free pH maintenance. No gas supplementation required.

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

Leibovitz's L-15 2X: 33 ingredients verified per lot with CAS numbers for full raw-material traceability. Leibovitz's L-15 uses D-galactose (not glucose) as the primary carbon source and high amino acid concentrations for CO₂-free pH buffering. This is a 2X concentrated formulation.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium chloride dihydrate	10035-04-8	370.000
Magnesium chloride hexahydrate	7791-18-6	400.000
Magnesium sulphate anhydrous	7487-88-9	195.440
Potassium chloride	7447-40-7	800.000
Potassium phosphate monobasic	7778-77-0	120.000
Sodium chloride	7647-14-5	16000.000
Sodium dihydrogen phosphate anhydrous	7558-80-7	380.240

Component	CAS Number	mg/L
AMINO ACIDS
L-Alanine	56-41-7	450.000
Glycine	56-40-6	400.000
L-Arginine (free base)	74-79-3	1000.000
L-Asparagine	70-47-3	500.000
L-Cysteine (free base)	52-90-4	240.000
L-Histidine (free base)	71-00-1	500.000
L-Isoleucine	73-32-5	500.000
L-Leucine	61-90-5	250.000
L-Lysine hydrochloride	657-27-2	188.000
L-Methionine	63-68-3	150.000
L-Phenylalanine	63-91-2	250.000
L-Serine	56-45-1	400.000
L-Threonine	72-19-5	600.000
L-Tryptophan	73-22-3	40.000
L-Tyrosine disodium salt	69847-45-6	552.320
L-Valine	72-18-4	200.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	2.000
D-Ca-Pantothenate	137-08-6	2.000
Folic acid	59-30-3	2.000
Nicotinamide	98-92-0	2.000
Pyridoxine hydrochloride	58-56-0	2.000
Riboflavin-5-phosphate sodium salt	130-40-5	0.200
Thiamine hydrochloride	67-03-8	2.000
i-Inositol	87-89-8	4.000
OTHERS
D-Galactose	59-23-4	1800.000
Phenol red sodium salt	34487-61-1	22.000

Custom formulation: Contact support@diagnocine.com for DCP-LL15-QP2X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 620.00–680.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Certificate of Analysis: Request for any DCP-LL15-QP2X lot at support@diagnocine.com.

Product Comparison

How DCP-LL15-QP2X compares

FluxMPS™ DCP-LL15-QP2X vs. conventional 0.22 µm–filtered Leibovitz's formulations.

Parameter	DCP-LL15-QP2X (FluxMPS™)	Conventional Leibovitz's L-1 (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
L-15 2X without L-Glutamine and Sodium Pyruvate — complete nitrogen and energy substrate control for CO₂-free culture	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 µm)	cancel No	cancel No
CO₂-free design	check_circle Yes (galactose)	check_circle Yes	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15-QP2X — Leibovitz's L-15 2X.

DCP-LL15-QP2X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size. Leibovitz L-15 CO₂-free design makes it ideal for chip loading, priming, open-stage imaging, and atmospheric OoC platforms where CO₂ control is impractical.

FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

Both nitrogen source (glutamine) and secondary carbon (pyruvate) are removed for independent fresh addition. This enables precise control of both energy substrate and nitrogen in CO₂-free primary cell dissociation, transport, and metabolic studies where individual contributions of glutamine and pyruvate need to be isolated.

CO₂-independent by design — Leibovitz's L-15 uses galactose and high amino acid buffering for CO₂-free pH maintenance. No gas supplementation required..

Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Contact support@diagnocine.com for custom co-formulation.

FluxMPS™ DCP-LL15-QP2X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For primary cells, endotoxin activates TLR4 altering cell phenotype and differentiation outcomes.

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting Leibovitz's L-15 2X in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ Leibovitz's L-15 Medium w/o L-Glutamine, Sodium Pyruvate: 2X Liquid