FluxMPS™ PBS, 10X (Phosphate Buffered Saline), Sterile

FluxMPS™ PBS, 10X (Phosphate Buffered Saline), Sterile

An MPS-grade, calcium- and magnesium-free phosphate buffered saline concentrate delivering precise, stable pH 7.4 and defined ionic strength across every dilution. Ultra-filtered through a quadruple-stage filtration architecture — 0.1 µm membrane filtered twice and 0.04 µm membrane filtered twice — and manufactured with ultrapure Type 1 water for microchannel-safe use across organ-on-a-chip and microfluidic workflows.

• Quadruple-stage filtration (0.1 µm membrane filtered twice and 0.04 µm membrane filtered twice) for microchannel-safe purity
• Endotoxin < 0.05 EU/mL by USP <85> Bacterial Endotoxin Test
• Precise pH 7.4 with defined 10X ionic strength (1370 mM NaCl, 27 mM KCl, 80 mM Na₂HPO₄, 20 mM KH₂PO₄)
• Ultrapure Type 1 water (18.2 MΩ·cm), ASTM 0.05 µS/cm
• ISO Class 5 (Class 100) aseptic fill and finish
• Calcium- and magnesium-free; protease-, DNase-, RNase-, and endonuclease-free
• Microchannel-compatible for OoC, ToC, BoC, and LoC platforms
• pH, molarity/concentration (1X–5X), and additive customization available on request

Engineered where standard buffers fail

Conventional 0.22 µm-filtered saline buffers leave behind subvisible particulates that accumulate in microchannels, drift in pH and ionic strength between lots, and carry endotoxin loads that raise assay background. FluxMPS™ PBS, 10X is formulated and filtered specifically to eliminate these failure modes for microfluidic and organ-on-a-chip (OoC) research.

Quadruple-stage filtration system

DCP-PBS10X is sterilized by a serial, four-stage sequential filtration process reaching 0.04 µm — the 0.1 µm membrane pass is performed twice and the 0.04 µm membrane pass is performed twice, all in a clean, controlled environment. This architecture removes large particulates and aggregates, retains fine particulates, bacteria, and mycoplasma-scale contaminants, and applies redundant second passes for ultra-pure, particle-free final polish.

1. 01

   0.1 µmPre-filtration I

   Large particulate and aggregate removal via 0.1 µm membrane filtration, guarding downstream filter life and chip geometries.

2. 02

   0.04 µmPre-filtration II

   Fine particulate, bacteria, and mycoplasma retention. Mycoplasma (typical diameter 0.1–0.3 µm) are retained at this stage — a key step absent in standard 0.22 µm filtration.

3. 03

   0.1 µmSterile-filtration I

   Second-pass removal of residual contaminants and bioburden, providing redundancy and ensuring no breakthrough from Stage 1.

4. 04

   0.04 µmSterile-filtration II — Final Polish

   Ultimate filtration for ultra-pure, particle-free, mycoplasma-free buffer. Final fill performed in a validated ISO Class 5 (Class 100) laminar-flow workstation under aseptic conditions.

Built for microphysiological systems research

DCP-PBS10X supports the full range of buffer needs across microfluidic, organ-on-a-chip, and traditional cell culture workflows, from chip priming and rinsing to dilution of stock reagents.

Automated Bioreactors & Robotics

For automated, closed-loop bioreactor and liquid-handling robotics platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of DCP-PBS10X is available on request, engineered to further protect microvalves, sensors, and long-duration perfusion lines from particulate fouling.

• Total Particulate Exclusion — sub-0.01 µm polish minimizes fine particulate carryover
• Valve & Sensor Protection — reduces fouling risk in automated microvalves and inline sensors
• Extended Perfusion Stability — supports longer unattended run times in closed perfusion loops

Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order — contact support@diagnocine.com to request this configuration.

Full specification summary

Key physical, chemical, purity, and regulatory parameters for DCP-PBS10X.

Full composition

All components below are expressed at 10X concentration exactly as released per lot; dilute to 1X for standard use. This is a single-group formulation with no source-defined sub-categories.

Manufacturing & compliance

DCP-PBS10X is manufactured, packaged, and quality-tested under a controlled, certified quality system.

How DCP-PBS10X compares

DCP-PBS10X against conventional 0.22 µm-filtered PBS and a standard commercial alternative.

Frequently asked questions

Common questions about DCP-PBS10X formulation, filtration, and customization.

Supporting literature

Curated peer-reviewed references relevant to PBS buffer chemistry, microfluidic perfusion, and organ-on-a-chip applications.

1. Bhatia, S. N. & Ingber, D. E. Microfluidic organs-on-chips. Nat. Biotechnol. 32, 760–772 (2014). doi:10.1038/nbt.2989
2. Halldorsson, S. et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens. Bioelectron. 63, 218–231 (2015). doi:10.1016/j.bios.2014.07.029
3. Good, N. E. et al. Hydrogen ion buffers for biological research. Biochemistry 5, 467–477 (1966). doi:10.1021/bi00866a011
4. Ferrell, N. et al. Vascular access buffer perfusion and ionic strength effects on endothelial cell monolayers. Ann. Biomed. Eng. 40, 1362–1373 (2012). doi:10.1007/s10439-011-0503-2
5. Malda, J. et al. Of mice, men and elephants: the relation between endotoxin biology and applications of cell-based technologies. Cytotherapy 21, 249–259 (2019). doi:10.1016/j.jcyt.2019.01.001
6. Low, L. A. et al. Organs-on-chips: into the next decade. Nat. Rev. Drug Discov. 20, 345–361 (2021). doi:10.1038/s41573-020-0079-3
7. United States Pharmacopeia. USP <788> Particulate Matter in Injections, Method 2 (Light Obscuration). USP-NF (2023). doi:10.31003/USPNF_M99715_02_01
8. Weber, E. J. et al. Application of a microfluidic kidney chip for evaluation of drug-induced nephrotoxicity. Sci. Rep. 8, 4941 (2018). doi:10.1038/s41598-018-23327-6