FluxMPS™ Ham's F-10 Nutrient Mixture with 25mM HEPES: 1X Liquid
FluxMPS™ Ham's F-10
FluxMPS™ DCP-H10H1X is an MPS-grade, ultra-filtered Ham's F-10 + 25mM HEPES formulation engineered for CHO cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering. Formulation: [+] 1100 mg/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES.
- Glucose: 1100 mg/L (1.1 g/L)
- 25 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering independent of CO₂
- Zinc, hypoxanthine, thymidine, lipoic acid — trace nutrients supporting CHO and primary fibroblast clonal growth
- 0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Media familyHam's F-10 + 25mM HEPES
- Glucose1100 mg/L (1.1 g/L)
- HEPES25 mM, pKa 7.3 at 37°C
- Formulation[+] 1100 mg/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate [+] 25 mM HEPES
- AppearanceRed-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Shelf Life12 months / 2–8°C
Engineered where standard media fails
Conventional 0.22 µm–filtered Ham's passes mycoplasma, subvisible particulates, and endotoxin fragments that clog microfluidic channels and activate innate immune signaling. FluxMPS™ eliminates these failure modes.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance. Safe perfusion in every chip geometry.
Cell-type–optimized formulation
Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid supporting CHO cell and primary fibroblast clonal growth.
Ultrapure-grade water
18.2 MΩ·cm — eliminates ionic contaminants that interfere with specialized cell culture requirements.
Below TLR4 endotoxin threshold
< 0.05 EU/mL — prevents LPS-driven inflammatory activation in primary cells.
HEPES pH stability
HEPES (25 mM) prevents pH rise during open-air handling, flow cytometry preparation, and atmospheric incubation.
Customization on demand
pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates; protects downstream 0.04 µm membranes.
-
2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
-
3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-H10H1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered Ham's, with confirmed mycoplasma barrier at every production stage.
© Diagnocine® — DCP-H10H1X
Specialized cell culture applications
FluxMPS™ DCP-H10H1X — Ham's F-10 + 25mM HEPES — delivers 0.04 µm filtered purity for CHO cells and related OoC applications.
Automated Bioreactors & Robotics
Optional 0.01 µm (10 nm) ultra-filtered variant available on request.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
- Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
CHO & Mammalian Cell Culture
Optimized for CHO, hybridoma, myeloma, and primary fibroblast culture in serum-free or low-serum conditions.
Organ-on-a-Chip & MPS
0.04 µm filtered media prevents microchannel clogging in complex multi-organ chip architectures.
Cancer Cell Lines
Supports fibroblast co-culture tumor models and primary cancer cell culture in the defined nutrient environment of specialized media.
Primary & Stem Cell Support
Rich nutrient profile with trace elements supports primary cell viability and stem cell maintenance in low-serum conditions.
Metabolic Flux Analysis
Defined nutrient base for ¹³C isotope tracing and Seahorse XF assays in specialized cell culture models.
Microscopy & Optical Sensing
Ultra-low particulate; phenol red–free variants for autofluorescence-free confocal and biosensor applications.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] 1100 mg/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES |
| Appearance | Red-colored, clear solution |
| Glucose | 1100 mg/L (1.1 g/L) |
| HEPES | 25 mM (pKa 7.3 at 37°C) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Total ingredients | 47 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | cold pack |
| CO₂ requirement | 5% CO₂ recommended (dual 25 mM HEPES + bicarbonate buffering; HEPES alone maintains pH without CO₂) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
Ham's F-10 + 25mM HEPES: 47 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid supporting CHO cell and primary fibroblast clonal growth.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 44.100 |
| Copper sulphate pentahydrate | 7758-99-8 | 0.0025 |
| Ferrous sulphate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride anhydrous | 7786-30-3 | 74.640 |
| Potassium chloride | 7447-40-7 | 285.000 |
| Potassium phosphate monobasic | 7778-77-0 | 83.000 |
| Sodium bicarbonate | 144-55-8 | 1200.000 |
| Sodium chloride | 7647-14-5 | 7400.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 153.700 |
| Zinc sulphate heptahydrate | 7446-20-0 | 0.0288 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 211.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 146.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 21.000 |
| L-Isoleucine | 73-32-5 | 2.600 |
| L-Leucine | 61-90-5 | 13.100 |
| L-Lysine hydrochloride | 657-27-2 | 29.300 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 11.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 3.570 |
| L-Tryptophan | 73-22-3 | 0.600 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 2.610 |
| L-Valine | 72-18-4 | 3.500 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.024 |
| Choline chloride | 67-48-1 | 0.698 |
| D-Ca-Pantothenate | 137-08-6 | 0.715 |
| Folic acid | 59-30-3 | 1.320 |
| Nicotinamide | 98-92-0 | 0.615 |
| Pyridoxal hydrochloride | 65-22-5 | 0.206 |
| Riboflavin | 83-88-5 | 0.376 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| Vitamin B12 | 68-19-9 | 1.360 |
| i-Inositol | 87-89-8 | 0.541 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1100.000 |
| HEPES buffer | 7365-45-9 | 5958.000 |
| Hypoxanthine Sodium Salt | 5765-44-6 | 4.080 |
| Lipoic acid | 1077-28-7 | 0.210 |
| Phenol red Sodium Salt | 34487-61-1 | 1.300 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm — eliminates ionic contaminants.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Target: Contact for specification.
Documentation & CoA
Full CoA with raw-material traceability available on request.
How DCP-H10H1X compares
FluxMPS™ DCP-H10H1X vs. conventional 0.22 µm–filtered Ham's formulations.
| Parameter | DCP-H10H1X (FluxMPS™) | Conventional Ham's F-10 + 25 (0.22 µm filtered) |
Standard DMEM/RPMI (0.22 µm filtered) |
|---|---|---|---|
| Ham's F-10 + 25mM HEPES — zinc, hypoxanthine, thymidine, lipoic acid for CHO and primary fibroblast OoC | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| HEPES (25 mM) | check_circle Yes | cancel Usually no | cancel No |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
Frequently asked questions
Common questions about FluxMPS™ DCP-H10H1X — Ham's F-10 + 25mM HEPES.
Supporting literature
Key publications supporting Ham's F-10 + 25mM HEPES in specialized cell culture and OoC applications.
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1083/jcb.1.3.273
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
