UltraClean GuardRNA DMSO Lysis Buffer
Cat No.:
DCP-GRNADLB
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe buffer for RNA handling, cell, and molecular biology experiments
Description:
GuardRNA DMSO Lysis Buffer is a ready-to-use, phenol-free lysis reagent formulated for efficient disruption of difficult or resilient samples while preserving RNA integrity. It combines a strong anionic detergent, high ionic strength, and dimethyl sulfoxide (DMSO) to enhance penetration and solubilization of tough cell walls and membranes, supporting high-yield, high-quality RNA extraction from challenging sample types.
Applications:
- Tough Sample RNA Extraction: Lysis of yeast, plant material, and other hard-to-lyse cells prior to RNA purification.
- Phenol-Free RNA Isolation: Primary lysis step in phenol-free workflows for samples that require enhanced solubilization.
- Mixed or Complex Samples: Processing of samples with high extracellular matrix content or partially fixed material where standard lysis is insufficient.
- Downstream RNA-seq, qPCR, and RT-PCR: Front-end lysis for difficult samples destined for expression profiling and other RNA-based assays (after appropriate cleanup).
- High-Throughput Workflows: Use in manual or automated pipelines that need a robust lysis reagent for diverse, challenging inputs.
Advantages:
- Enhanced Lysis of Tough Samples: DMSO improves penetration and solubilization, increasing lysis efficiency for hard-to-disrupt cells and tissues.
- Strong RNase Inactivation: Detergent and high-salt conditions rapidly denature proteins, including RNases, helping maintain RNA integrity during extraction.
- Phenol-Free Workflow: Avoids phenol/chloroform extraction, simplifying handling and waste disposal.
- Ready-to-Use: Supplied as a 1X solution prepared with RNase-free water, reducing preparation time and contamination risk.
- Broad Compatibility: Integrates with common downstream RNA purification formats, including columns, magnetic beads, and precipitation methods.
Composition:
| Ingredients |
Concentration |
| Sodium Dodecyl Sulfate (SDS) |
4% (w/v) |
| Sodium Chloride (NaCl) |
0.5 M |
| Dimethyl Sulfoxide (DMSO) |
2% (v/v) |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
