GSTP1 Green

Product#: FDV-0034
$400.00
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GSTP1 Green


Cat No: FDV-0034
 
Size: 1 kit (0.1 mg)
Storage: -20°C
Formulation: C30H22N2O9S
Molecular weight: 586.6 g/mol
Solubility: Soluble in DMSO
Ex/Em: 493/510 nm

Description
 
GSTP1 Green is a novel fluorogenic GSTP1 (Pi-class Glutathione S-Transferase) specific substrate for live cell imaging. Glutathione S Transferases (GSTs) are widely conserved in nature from bacteria to plants and animals. In human, over 20 members are identified and classified into three subfamilies cytosolic, mitochondrial and membrane bound microsomal members. The cytosolic subfamily consists of seven classes including alpha, mu, omega, pi, theta, sigma and zeta. GSTs play an important role in detoxification of hydrophobic and electrophilic compounds including endogenous toxic metabolites or xenobiotics by conjugating with glutathione (GSH) to produce glutathione conjugates (GS-conjugates). 

GS-conjugates released from GSTs are quickly exported to extracellu lar space by multidrug resistance associated protein (MRP) transporters. Through these processes, GSTs detoxify toxic compounds. Among the cytosolic GST subfamily, Pi class GST (GSTP1) is the most studied member because GSTP1 is highly expressed in various cancer cells. GSTP1 is considered as a major enzyme for anticancer drug resistance in malignant cancer cells through the neutralization of drugs . To understand biological functions of GSTP1 , research tools for specifically monitoring GSTP1 activity are required . Although several reagents including CDNB having broad specificity for GST family have been developed, no tools to analyze GSTP1 activity was commercially available.  

"GSTP1 Green" (original name in Ref.1 “Ps-TAc") is a specific fluorogenic substrate for GSTP1 developed by Dr. Yuuta Fujikawa, Tokyo University of Pharmacy and Life Sciences. "GSTP1 Green" is valuable probe allowing to monitor cellular GSTP1 activity under any extracellular stimuli. in live cell. 

This product has been commercialized under the license from Tokyo University of Pharmacy and Life Sciences.

Principle
 
FDV-0034_fig1.png 

Features
  • Highly spefic for GSTP1
  • "GSTP1 Green" is quenched before GSTP1 enzymatic reaction.
  • Ex./Em. = 493 nm / 510 nm. (Compatible with FITC filter)
  • For live cell imaging only. (Not applicable to in vivo assays)
Data 
 
FDV-0034_fig2.png 
Fig.1 Specificity of "GSTP1 Green" for 18 GST family members
MCF7, a GSTP1 low expressing cell line, was transfected with plasmids encoding 18 human GST members.

Each GST subt ype overexpressed MCF7 cell was treated with 2.5 μM of GSTP1 Green for 5 min and observed green
fluorescence (Ex. 473 nm / Detection; FITC filter). Only GSTP1 overexpressed cells showed green fluorescence .
This indicates "GSTP1 Green" is highly specific for GSTP1 among wide GST members.

FDV-0034_fig3.png 
Fig.2 Kinetic analysis of cellular GSTP1 activity
Two cancer cells, HT1080 which is a GSTP1 highly expressing cell line and LNCaP which is a GSTP1 low
expressing cell line , were treated with 2.5 μM of "GSTP1 Green" and monitored fluorescent intensity

(Ex. 473 nm / Detection; FITC filter) for 20 min.

Application
  • Cell-based GSTP1 activity assay in live cells
Reconstitution and Storage
 
Reconstitution Both “GSTP1 Green” and MK571 are reconstituted in 100% DMSO.
Storage (solution) Store powder at -20oC. After reconstitution in DMSO, aliquot and store at -20 °C. Avoid repeated freeze-thaw cycles.

Reference
  1. Mori et al., Chem. Commun., 55, 8122~8125 (2019), A highly selective fluorogenic substrate for imaging glutathione S-transferase 1:development, cellular applicability to epigenetic studies.  PMID : 31237279
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