Formaldehyde Gel-Loading Buffer [10X]
FluxMPS™ Formaldehyde Gel-Loading Buffer [10X]
An MPS-grade, quadruple-stage-filtered 10X loading buffer engineered for denaturing RNA gel electrophoresis. Glycerol density, dual tracking dyes, and EDTA chelation are combined in an ultra-clean, nuclease-free formulation validated for reliable RNA size separation on formaldehyde agarose gels and microchannel-safe workflows.
- Filtered 0.1 µm membrane twice and 0.04 µm membrane twice (quadruple-stage architecture)
- No DNase or RNase activity detected after 18 hr incubation at room temperature
- Stable pH 8.0 formulation with dual bromophenol blue / xylene cyanol FF tracking dyes
- 50% glycerol content for accurate, loss-free well loading
- Manufactured with ultrapure Type 1 water (18.2 MΩ·cm)
- Produced under ISO 13485:2016 QMS in CE-approved facilities
- pH, dye selection, and additive concentration customizable on request
- pH8.0
- EDTA Concentration10 mM
- Glycerol Content50%
- Tracking DyesBromophenol blue 0.025% + Xylene cyanol FF 0.025%
- Filtration0.1 µm x2 + 0.04 µm x2
- SterilityFilter-sterilized, sterile environment
- Nuclease ActivityNone detected (DNase/RNase)
- Water QualityUltrapure Type 1, 18.2 MΩ·cm
- Storage-20°C
- Shelf Life1 year
Engineered where standard loading buffers fail
Conventional 0.22 µm-filtered loading buffers can carry subvisible particulate and residual nuclease activity that compromise RNA integrity during denaturing electrophoresis. The FluxMPS™ buffer line applies deeper filtration and defined chemistry to reduce these risks at the source.
Microchannel-safe purity
Sequential 0.1 µm and 0.04 µm membrane passes reduce particulate that can interfere with fine gel wells and downstream imaging.
Precise, stable pH
Formulated to pH 8.0 to support consistent RNA denaturation and reproducible migration on formaldehyde agarose gels.
Ultrapure-grade water
Prepared with ultrapure Type 1 water (18.2 MΩ·cm) per USP <85> water-quality practice.
Low background for imaging & assays
Bromophenol blue and xylene cyanol FF tracking dyes give clean, well-separated visual markers without added background staining.
Defined, traceable composition
Glycerol, EDTA, and dual tracking dyes are formulated to stated concentrations and confirmed against lot-level QC testing.
Customization on demand
Alternate dye systems, concentrations, or pH are available — contact support@diagnocine.com.
Quadruple-stage filtration system
DCP-FGL10X is filtered 0.1 µm membrane twice and 0.04 µm membrane twice, in a sterile environment, before final fill.
-
1
0.1 µm Pre-filtration I
First-pass 0.1 µm membrane removes large particulate and aggregates ahead of downstream filtration.
-
2
0.04 µm Pre-filtration II
0.04 µm membrane pass retains fine particulates and bioburden, including organisms in the mycoplasma size range (approximately 0.2 micron).
-
3
0.1 µm Sterile-filtration I
Second 0.1 µm pass provides redundant clarification ahead of final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
Final 0.04 µm pass is performed in a sterile fill environment for the finished buffer.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, applied twice each, removes finer particulates and reduces bioburden risk beyond what a single 0.22 µm pass achieves.
© Diagnocine® — DCP-FGL10X
Where DCP-FGL10X is used
A dedicated loading buffer for denaturing RNA gel electrophoresis, Northern blotting, and RNA integrity assessment, with formulation flexibility for related molecular biology workflows.
Automated Bioreactors & Robotics
For automated liquid-handling and robotic RNA sample-prep platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request to further reduce particulate exposure to valves, sensors, and dispense tips.
- Total Particulate Exclusion: Finer sub-0.04 µm filtration for automated pipetting lines
- Valve & Sensor Protection: Reduced particulate load protects microvalves and optical sensors
- Extended Perfusion Stability: Lower particulate carryover supports longer unattended run cycles
Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-clean loading buffer suitable for RNA sample handling adjacent to microfluidic and chip-based workflows.
RNA Sample Loading & Preparation
Increases sample density and denatures RNA for accurate well loading ahead of gel electrophoresis.
iPSC-Derived Model RNA Analysis
Compatible with RNA integrity and expression workflows on iPSC-derived model systems.
Endothelial & Primary Cell RNA Profiling
Supports RNA sample prep from endothelial and primary cell perfusion cultures for gel-based analysis.
Northern Blot Analysis
Suitable for preparing RNA samples ahead of Northern blot hybridization to membrane.
Pre-rRNA & rRNA Visualization
Dual tracking dyes allow visual monitoring of high molecular weight RNA species during electrophoresis.
Full parameter breakdown
Measured and declared values for DCP-FGL10X, as released.
| Parameter | Specification |
|---|---|
| Formulation | Glycerol, bromophenol blue, xylene cyanol FF, EDTA — see the full composition table below |
| Appearance | Blue-purple or violet color |
| pH USP <791> | 8.0 |
| EDTA concentration | 10 mM |
| Glycerol content | 50% |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase activity | None detected after 18 hr incubation with plasmid DNA at room temperature |
| RNase activity | None detected after 18 hr incubation with ribosomal RNA at room temperature |
| Water quality | Ultrapure Type 1 water, 18.2 MΩ·cm |
| Manufacturing standard ISO 13485 | ISO 13485-certified facility |
| Parameter | Specification |
|---|---|
| Storage temperature | -20°C |
| Shelf life | 1 year |
| Unit size | 2 x 1 mL |
| Parameter | Specification |
|---|---|
| Regulatory alignment | Manufactured under ISO 13485-certified and CE-approved facilities |
| Final QA & packaging | DiagnoCine R&D and Quality Testing Center |
| Custom assembly location | DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended use | Research Use Only (RUO) |
Full composition
Component list for the 10X Formaldehyde Gel-Loading Buffer, reported by ingredient and concentration.
| Component | CAS Number | Concentration |
|---|---|---|
| Glycerol | 56-81-5 | 50% |
| Bromophenol blue | 115-39-9 | 0.025% |
| Xylene cyanol FF | 2650-17-1 | 0.025% |
| EDTA | 60-00-4 | 10 mM |
Manufacturing & compliance
DCP-FGL10X is produced, filtered, and released under a controlled quality system.
ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified, CE-approved facility.
Ultrapure Type 1 Water
Formulated with ultrapure Type 1 water (18.2 MΩ·cm).
ISO Class 5 Fill & Finish
Final fill performed in a sterile filtration environment.
Micro-Batch Precision
Custom assembly and packaging completed at DiagnoCine Precision, Totowa, New Jersey, USA.
Nuclease Testing
No DNase or RNase activity detected after 18 hr incubation at room temperature.
Sterility USP <71>
Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment.
pH Verification USP <791>
Released at pH 8.0.
Documentation / CoA
Lot-specific Certificate of Analysis available on request.
How DCP-FGL10X compares
A side-by-side view of filtration architecture and quality attributes against conventional loading buffers.
| Parameter | DCP-FGL10X (FluxMPS™) | Conventional 0.22 µm Buffer | Standard Alternative |
|---|---|---|---|
| Nuclease-tested (DNase/RNase) | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Sterility tested (USP <71>) | check_circle | cancel | check_circle |
| Water quality | Ultrapure Type 1 | Deionized | Deionized |
| Manufacturing QMS | ISO 13485:2016 | Not specified | Not specified |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-FGL10X.
Supporting literature
Curated references relevant to denaturing RNA gel electrophoresis and buffer purity practices.
- Masek T, Vopalensky V, Suchomelova P, Pospisek M. Denaturing RNA electrophoresis in TAE agarose gels. Anal Biochem. 2005. doi:10.1016/j.ab.2005.07.018
- Streit S, Michalski CW, Erkan M, Kleeff J, Friess H. Northern blot analysis for detection and quantification of RNA in pancreatic cancer research. Nat Protoc. 2009. doi:10.1038/nprot.2008.213
- Farrell RE. RNA Methodologies: A Laboratory Guide for Isolation and Characterization. Academic Press. doi:10.1016/C2009-0-64150-6
- Nielsen H (ed). RNA Detection: Methods and Protocols. Methods Mol Biol. 2018. doi:10.1007/978-1-4939-7213-5
- Aranda PS, LaJoie DM, Jorcyk CL. Bleach gel: a simple agarose gel for analyzing RNA quality. Electrophoresis. 2012. doi:10.1002/elps.201200335
- Kang M, Ko E, Mersha TB. A roadmap for multi-omics data integration using deep learning. Brief Bioinform. 2022 (background on RNA workflow QC relevant to gel loading practices). doi:10.1093/bib/bbab454
- Volkin E, Cohn WE. On the structure of ribonucleic acids. J Biol Chem. Foundational RNA biochemistry relevant to EDTA-based nuclease protection. doi:10.1016/S0021-9258(18)57109-0
- Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998. doi:10.1128/MMBR.62.4.1094-1156.1998
- Uphoff CC, Drexler HG. Detection of mycoplasma contaminations. Methods Mol Biol. 2011. doi:10.1007/978-1-61779-108-6_1
