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- FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), Low Glucose & 50mM HEPES w/o L-Glutamine, Phenol Red: 2X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), Low Glucose & 50mM HEPES w/o L-Glutamine, Phenol Red: 2X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose & 50mM HEPES w/o L-Glutamine, Phenol Red: 2X Liquid
Double-strength (2X) DMEM Low Glucose with 50 mM HEPES — 2.0 g/L glucose and 50 mM HEPES in the concentrate, 1.0 g/L and 25 mM at 1X — for semisolid, colony-formation, 3D and metabolic studies that need a lower-glucose environment, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate + 50 mM HEPES dual buffer and no Phenol Red, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: mix with an equal volume of a compatible solution or matrix for a final 1X medium
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- 50 mM HEPES (25 mM at 1X) for CO₂-independent pH stability during matrix preparation and handling
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 50mM HEPES; [−] L-Glutamine, [−] Phenol Red
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-Glucose2,000 mg/L
- L-GlutamineNot included
- Sodium Pyruvate220 mg/L
- BufferNaHCO₃ 7,400 mg/L + 50 mM HEPES
- Phenol RedNot included
- AppearancePale Yellow
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.
Total metabolic control
Defined 2,000 mg/L D-glucose, no L-glutamine and 220 mg/L sodium pyruvate in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing, and the Phenol Red-free formulation removes color background.
Matrix-ready 2X strength
32 components at double strength, so agar, collagen, hydrogels or treatment solutions can be added 1:1 without diluting the medium below 1X.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-DMEMLH-QR2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
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02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
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03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
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04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-DMEMLH-QR2X
Where FluxMPS™ DMEM fits
DMEM Low Glucose is a widely used basal medium containing 1.0 g/L glucose at its standard 1X working concentration.[1,2] It supports the culture of many primary and established mammalian cells and is commonly used for fibroblast culture, stem-cell research, metabolic studies, tissue engineering, and other applications that require a lower-glucose environment.[3]
DMEM Low Glucose 2X is a double-strength formulation designed to be diluted to 1X or combined with an equal volume of another compatible solution while maintaining the intended final concentrations of glucose, amino acids, vitamins, and salts. The 2X concentrate contains 2.0 g/L glucose and provides 1.0 g/L glucose after a 1:1 dilution.
Researchers may use the 2X formulation when incorporating large volumes of agar, agarose, methylcellulose, collagen, hydrogels, treatment solutions, supplements, or cell suspensions that would otherwise dilute standard 1X medium. It is useful for semisolid cultures, colony-formation assays, 3D cell culture, tissue engineering, metabolic research, and specialized cell-based assays.[4,5]
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Colony-Formation Assays
Mix 1:1 with molten agar, agarose or methylcellulose for colony-formation and anchorage-independent growth assays at 1X medium strength.[4]
Collagen-Gel & Hydrogel Cultures
Fold in collagen, methylcellulose or hydrogels for gel-contraction studies and 3D constructs.[5]
Metabolic Research
1.0 g/L glucose at 1X (about 5.5 mM) sits close to physiological blood glucose, a common baseline for metabolic, glycolysis and insulin-signaling studies.
MSCs, Fibroblasts & Primary Cells
Low-glucose DMEM is a standard base for mesenchymal stem cell expansion, fibroblast culture and other primary cells after dilution to 1X.[3]
Organ-on-a-Chip & MPS
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]
Microscopy & Optical Sensing
Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors; no Phenol Red background.
Specifications & quality control
Release specifications for DCP-DMEMLH-QR2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 50mM HEPES; [−] L-Glutamine, [−] Phenol Red |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Pale Yellow |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | 2,000 mg/L (2.0 g/L; 1.0 g/L at 1X) |
| L-Glutamine | Not included |
| Sodium Pyruvate | 220 mg/L |
| Sodium Bicarbonate | 7,400 mg/L |
| Phenol Red | Not included |
| HEPES | 11,916 mg/L (50 mM; 25 mM at 1X) |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | CO₂-controlled incubator recommended after dilution to 1X (sodium bicarbonate 7,400 mg/L in the 2X, 3,700 mg/L at 1X); 50 mM HEPES (25 mM at 1X) steadies pH during handling outside the incubator |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-DMEMLH-QR2X is 2X DMEM with 2.0 g/L glucose, sodium pyruvate, sodium bicarbonate and 50 mM HEPES. It does not contain L-glutamine nor Phenol Red. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 32 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 530.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.200 |
| Magnesium sulphate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Sodium bicarbonate | 144-55-8 | 7400.000 |
| Sodium chloride | 7647-14-5 | 12800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 218.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 60.000 |
| L-Arginine hydrochloride | 1119-34-2 | 168.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 125.140 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 84.000 |
| L-Isoleucine | 73-32-5 | 210.000 |
| L-Leucine | 61-90-5 | 210.000 |
| L-Lysine hydrochloride | 657-27-2 | 292.000 |
| L-Methionine | 63-68-3 | 60.000 |
| L-Phenylalanine | 63-91-2 | 132.000 |
| L-Serine | 56-45-1 | 84.000 |
| L-Threonine | 72-19-5 | 190.00 |
| L-Tryptophan | 73-22-3 | 32.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 207.580 |
| L-Valine | 72-18-4 | 188.00 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 8.000 |
| D-Ca-Pantothenate | 137-08-6 | 8.000 |
| Folic acid | 59-30-3 | 8.000 |
| Nicotinamide | 98-92-0 | 8.000 |
| Pyridoxine hydrochloride | 58-56-0 | 8.000 |
| Riboflavin | 83-88-5 | 0.800 |
| Thiamine hydrochloride | 67-03-8 | 8.000 |
| i-Inositol | 87-89-8 | 14.400 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Sodium pyruvate | 113-24-6 | 220.000 |
| HEPES | 7365-45-9 | 11916.000 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-DMEMLH-QR2X compares
Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-DMEMLH-QR2X (FluxMPS™) | Conventional DMEM Low Glucose + HEPES, 1X (0.22 µm filtered) | Conventional 2X DMEM (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X DMEM Low Glucose + 50 mM HEPES; free of L-Glutamine and Phenol Red | 1X DMEM, ready to use | Double-strength, for semisolid culture |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-DMEMLH-QR2X for semisolid, 3D, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on DMEM, HEPES buffering, mesenchymal stem cell culture, semisolid and collagen-gel culture, Phenol Red, organ-on-a-chip culture and mycoplasma control.
- Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143–147. doi:10.1126/science.284.5411.143
- Borowicz S, Van Scoyk M, Avasarala S, et al. The soft agar colony formation assay. J Vis Exp. 2014;(92):e51998. doi:10.3791/51998
- Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA. 1979;76(3):1274–1278. doi:10.1073/pnas.76.3.1274
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
- Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
