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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-DMEMH-B1X is an MPS-grade, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems, and CO₂-independent or open-top microfluidic platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Sodium Bicarbonate.
- High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and aerobically active cells
- 25 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering independent of CO₂ tension
- 0.04 µm final nano-filtration — sub-mycoplasma purity for microfluidic channels below 100 µm
- Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
- Endotoxin < 0.05 EU/mL (LAL, USP <85>)
- Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
- Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
- Glucose4500 mg/L (4.5 g/L, High Glucose)
- HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
- Formulation[+] High Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES, [-] Sodium Bicarbonate
- AppearanceOrange-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)250.00–290.00 mOsm/kg H₂O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, away from light
- Shelf Life12 months
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma, subvisible particulates, and endotoxin fragments that clog microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both failure modes simultaneously.
Microchannel-safe purity
0.04 µm final filtration; USP <788> particulate compliance ensures safe perfusion in every chip geometry, including sub-100 µm channels.
High-energy cell support
4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.
HEPES: CO₂-stable pH
25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 regardless of CO₂ fluctuation — critical for open-top chips, point-of-care devices, and atmospheric incubation.
Low background for imaging
Ultra-low particulate; phenol red–free variants eliminate autofluorescence for confocal and biosensor platforms on chip.
Rich, stable nutrient profile
32 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.
Customization on demand
pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma purity unavailable from conventional 0.22 µm media.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
Removes large aggregates and contaminants; protects downstream 0.04 µm membranes and chip geometries.
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2
0.04 µm Pre-filtration II — Mycoplasma Barrier
Retains mycoplasma (0.1–0.3 µm) and fine particulates — absent from standard 0.22 µm filtration.
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3
0.1 µm Sterile-filtration I — Second-pass Redundancy
Second-pass sterility redundancy; no breakthrough from Stage 1.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
FluxMPS™ DCP-DMEMH-B1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered DMEM, with HEPES buffering for pH stability and confirmed mycoplasma barrier at every production stage.
© Diagnocine® — DCP-DMEMH-B1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEMH-B1X combines high-glucose energy support with HEPES pH stability — supporting demanding platforms from open-top microfluidic chips and CO₂-free bioreactors to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
HEPES buffering eliminates CO₂ dependency in automated bioreactor perfusion. An optional 0.01 µm (10 nm) ultra-filtered variant is available on request for robotic liquid handlers where even trace particulates cause valve failure.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates from bioreactor media lines
- pH-Stable Automated Delivery: HEPES maintains pH during robotic media exchanges without CO₂ re-equilibration delays
- Extended Perfusion Stability: Consistent high-glucose delivery with stable pH over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.
Open-Top & CO₂-Free Chips
HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO₂ environments.
Primary Neurons & Brain-on-Chip
High glucose (4.5 g/L) + HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion without pH drift.
Cardiomyocyte & Heart-on-Chip
High-glucose DMEM + HEPES is the standard base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.
Warburg Effect & Cancer Models
High glucose supports aerobic glycolysis in cancer lines; HEPES stabilizes pH during rapid glucose consumption spikes in Warburg-active tumour models.
Metabolic Flux Analysis
Defined high-glucose formulation for ¹³C isotope tracing and Seahorse XF assays; HEPES allows sampling outside incubators without pH artefacts.
Microscopy & Optical Sensing
HEPES-stabilized pH during imaging sessions; phenol red–free variants deliver zero autofluorescence for confocal and biosensor platforms.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] 25mM HEPES | [-] Sodium Bicarbonate |
| Appearance | Orange-colored, clear solution |
| Glucose | 4500 mg/L (4.5 g/L, High Glucose) |
| HEPES | 25 mM (5958 mg/L), pKa 7.3 at 37°C |
| pH USP <791> | 7.4 ± 0.04 |
| Osmolality USP <785> | 250.00–290.00 mOsm/kg H₂O |
| Total ingredients | 32 |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | Negative (0.04 µm barrier) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 32 ingredients verified per lot with CAS numbers for full raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the OTHERS tab.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 4500.000 |
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA and testing at DiagnoCine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, USP <85> resistivity — eliminates ionic contaminants affecting cell signaling and sensor measurements.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, ensuring container-closure integrity.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.
Endotoxin — USP <85> BET
LAL assay, release limit: < 0.05 EU/mL per lot.
Particulate — USP <788> Method 2
Light obscuration: ≤25/mL (≥10 µm), ≤3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 250.00–290.00 mOsm/kg H₂O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
How DCP-DMEMH-B1X compares
FluxMPS™ DCP-DMEMH-B1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.
| Parameter | DCP-DMEMH-B1X (FluxMPS™) | Conventional DMEM HG (0.22 µm filtered) |
Standard DMEM HG + HEPES (0.22 µm filtered) |
|---|---|---|---|
| HEPES-only buffering — fully CO₂-independent, atmospheric incubation compatible | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma barrier | check_circle Yes (0.04 µm) | cancel No | cancel No |
| HEPES buffer (25 mM) | check_circle Yes | cancel No | check_circle Yes |
| Endotoxin specification | < 0.05 EU/mL | NMT 1 EU/mL | NMT 1 EU/mL |
| USP <788> particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatible | check_circle MPS-grade | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEMH-B1X DMEM High Glucose + 25mM HEPES.
Supporting literature
Key peer-reviewed publications supporting MPS-grade, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
