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- FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), Low Glucose w/o L-Glutamine, Phenol Red: 1X Liquid
FluxMPS™ DCP-DMEML-QR1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Low Glucose DMEM engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. The formulation omits L-Glutamine and Phenol Red for researcher-controlled nitrogen addition and a clean optical/hormonal baseline, while retaining Sodium Pyruvate and a Sodium Bicarbonate buffering system. Formulation: [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red.
- Quadruple-stage filtration train: 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm, reaching a 0.04 µm final cut-off for microchannel-safe perfusion
- Low Glucose (1000 mg/L / 1.0 g/L) base with Sodium Pyruvate (110 mg/L) for controlled carbon-source and Warburg-effect metabolic studies
- Formulated without L-Glutamine (add fresh at time of use) and without Phenol Red — supports hormone-sensitive assays and clean fluorescence/biosensor imaging on chip
- Sodium Bicarbonate (3700 mg/L) buffering system; requires an incubator atmosphere of approximately 10% CO₂ to maintain pH 7.4
- Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system; Certificate of Analysis available on request
- Microfluidics Suitable grade (0.04 µm final cut-off) for organ-on-a-chip (OoC), microphysiological systems (MPS), and tissue-chip culture
- Custom pH, glucose, salts, and nutrient adjustments available on request
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- L-GlutamineNot included
- Sodium Pyruvate110 mg/L
- pH (USP <791>)7.4
- Osmolality (USP <785>)310–350 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from manufacture, unopened
- ShippingCold pack
Engineered where standard media fails
Conventional 0.22 µm–filtered DMEM passes mycoplasma-scale organisms (0.2–0.3 µm diameter) and subvisible particulates that accumulate inside microfluidic channels, clog chip geometries, and corrupt sensor readings. FluxMPS™ was designed specifically for these failure modes.
Microchannel-safe purity
0.04 µm final filtration removes sub-micron particles that clog channels below 100 µm. USP <788> Method 1 particulate compliance supports safe perfusion in narrow chip geometries.
Total metabolic control
Low glucose (1.0 g/L) base with user-defined glutamine, Sodium Pyruvate, and Sodium Bicarbonate allows precise definition of carbon and nitrogen sources for Warburg-effect and metabolic flux studies.
Ultrapure-grade water
Prepared using Ultrapure Type 1 water (18.2 MΩ·cm), controlled for trace metals and total organic carbon (TOC) to minimize non-nutrient background in every batch.
Low background for imaging
Ultra-low particulate baseline and a phenol red–free formulation (no autofluorescent indicator dye) support cleaner confocal, widefield, and biosensor measurements on chip.
Rich, stable nutrient profile
4× BME amino acid & vitamin concentrations, micro-batch manufacturing, and per-lot QC support reproducible cell growth across multi-day perfusion experiments.
Customization on demand
pH, glucose concentration, salts, HEPES, and nutrient composition are adjustable per your protocol. Contact support@diagnocine.com to specify your formulation.
Quadruple-stage filtration system
Four serial filtration passes reach a final 0.04 µm polish: two dedicated prefilter + final-filter pairs run in series, each 0.04 µm cartridge protected by its own 0.1 µm prefilter.
-
1
0.1 µm Prefiltration I
Removes large aggregates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and extends its service life.
-
2
0.04 µm Final filtration I
Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge from early fouling.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter prior to aseptic fill & finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
DCP-DMEML-QR1X runs two prefilter + final-filter pairs in series, giving redundant particulate exclusion ahead of aseptic fill.
passes (paired 0.1 µm
+ 0.04 µm stages)
pore size
© Diagnocine® — DCP-DMEML-QR1X
Designed for next-generation cell models
FluxMPS™ DCP-DMEML-QR1X supports demanding cell culture platforms — from single-channel microfluidic chips to multi-organ body-on-a-chip systems — where media purity, metabolic precision, and optical clarity matter.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated bioreactor perfusion systems, robotic liquid handlers, and long-duration closed-loop platforms where trace particulates cause valve failure or sensor drift.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates invisible to standard QC methods
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves, peristaltic pump tubing, and inline optical sensors
- Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture without filter replacement in the chip circuit
Inquiry Required: The 0.01 µm MPS Grade variant is a custom product. Contact support@diagnocine.com to request it for your automated system.
Micro Physiological System (MPS) & Chip
Ultra-clean, 0.04 µm–filtered media supports laminar flow fidelity in complex multi-organ chip architectures.
Warburg Effect & Metabolic Research
Low glucose (1.0 g/L) base with user-defined nitrogen sources enables control of aerobic glycolysis for Warburg phenotype studies.
iPSC-Derived Models
Low endotoxin specification (< 0.05 EU/mL) and mycoplasma-retentive filtration support sensitive iPSC-differentiation protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled DMEM supports HUVEC and primary hepatocyte monolayer integrity for barrier-function assays and TEER monitoring.
Metabolic Flux Analysis
Defined low-glucose formulation with omitted glutamine provides a controlled metabolic background for 13C isotope tracing and extracellular flux measurement. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate count and a phenol red–free formulation (no autofluorescent indicator dye) provide a clean baseline for confocal imaging, biosensor arrays, and TEER measurements on chip.
Analytical release specifications
Every lot is released against the specification matrix below. CoA available on request: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Sodium Bicarbonate, [+] Calcium, [+] Magnesium, [+] Glucose, [+] Sodium Pyruvate | [-] L-Glutamine, [-] Phenol Red |
| Appearance | Colorless to pale yellow, clear solution (no phenol red indicator) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310–350 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| L-Glutamine | Not included |
| Sodium Pyruvate | 110 mg/L |
| Phenol Red | Not included |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> | NMT 25/mL |
| Particulate ≥25 µm USP <788> | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | ≈10% CO₂ incubator (derived from 3700 mg/L sodium bicarbonate to maintain pH 7.4) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Available pack sizes | 500 mL, 1000 mL |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
FluxMPS™ DMEM is a modification of Basal Medium Eagle (BME) containing 4× BME concentrations of amino acids and vitamins, plus glycine, serine, and ferric nitrate. Total: 31 components, organized into 3 categories: Inorganic Salts, Amino Acids, and Vitamins & Others. CAS numbers provided for traceability.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-15-0 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Sodium pyruvate | 113-24-6 | 110.000 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality management system. Final packaging, QA, and testing are conducted at the Diagnocine R&D and Quality Testing Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
All formulations use Ultrapure Type 1 water (18.2 MΩ·cm), controlled for trace metals and total organic carbon to minimize non-nutrient background.
ISO Class 5 Fill & Finish
Final aseptic fill performed in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity at the point of fill.
Micro-Batch Precision
Small-batch production with full per-lot traceability. Each batch is individually tested and released — no blending of lots, no averaging of QC results. A Certificate of Analysis is issued for every batch.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay. Release specification: < 0.05 EU/mL, tested per manufacturing batch.
Particulate — USP <788> Method 1
Light obscuration particle count. Release limits: ≤25/mL (≥10 µm) and ≤3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target range: 310–350 mOsm/kg H2O.
Documentation & CoA
Full Certificate of Analysis with raw-material traceability, in-process records, and final-release test results available for every batch upon request.
How DCP-DMEML-QR1X compares
FluxMPS™ DCP-DMEML-QR1X vs. conventional 0.22 µm–filtered DMEM formulations.
| Parameter | DCP-DMEML-QR1X (FluxMPS™) | Conventional DMEM (0.22 µm filtered) |
Standard Alt. DMEM (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade | Standard grade |
| No L-Glutamine and no Phenol Red — researcher-controlled nitrogen addition and clean optical baseline | check_circle Yes | cancel No | cancel No |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel Not tested | cancel Not tested |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed formulation | cancel Fixed formulation |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-DMEML-QR1X.
Supporting literature
Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip, microfluidic, and metabolic research applications.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
