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- FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol: 2X Liquid
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol: 2X Liquid
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol: 2X Liquid
Double-strength (2X) Click's Medium (EHAA) for murine T cells, lymphocytes, hybridomas and other immune-cell models, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate buffer and no 2-mercaptoethanol, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: mix with an equal volume of another solution for a final 1X nutrient concentration
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- Endotoxin < 0.05 EU/mL (USP <85> BET) — important for LPS-sensitive immune cells
- Formulation: [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] L-Glutamine, [−] Mercaptoethanol
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- Glucose2,000 mg/L
- L-GlutamineNot included
- Sodium Pyruvate550 mg/L
- BufferNaHCO₃ 2,700 mg/L
- 2-MercaptoethanolNot included
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8 °C, protect from light
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional Click's Medium is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels and add noise to optical readouts — and mycoplasma is a known confounder of lymphocyte and hybridoma work.[10,11] FluxMPS™ Click's Medium keeps the EHAA formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing and porous membranes.
Total metabolic control
Defined 2,000 mg/L glucose, 550 mg/L sodium pyruvate and no L-glutamine in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in flow cytometry, confocal imaging and on-chip sensing.
Nucleoside-enriched EHAA base
42 components, including Eagle's amino acids with Hanks' salts plus adenosine, cytosine, guanosine and uridine.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-CM-Q2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X Click's Medium polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
-
02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
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03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
-
04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ Click's Medium is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-CM-Q2X
Where FluxMPS™ Click's Medium fits
Click’s Medium (EHAA) is a specialized cell culture medium developed for studying immune responses in mouse spleen cells.[1,2] It is commonly used for the culture of murine T cells, lymphocytes, hybridomas, and other immune-cell models in immunology, antibody, and cell-based research.[3]
Click’s Medium (EHAA) 2X is a double-strength formulation designed for research workflows that require the medium to be mixed with an equal volume of another solution while maintaining a final 1X nutrient concentration. It is useful for preparing customized media, incorporating supplements or treatment solutions, and supporting specialized T-cell, lymphocyte, hybridoma, and other immune-cell culture applications.
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Murine T Cells & Lymphocytes
Culture of mouse spleen cells, T cells and other lymphocytes for in vitro immune-response, activation and proliferation studies.[1,2]
Hybridoma Culture
Base medium for hybridoma growth and antibody production workflows, with serum or defined supplements.[3]
Immune-on-Chip & MPS
Microchannel-safe medium for perfused immune-cell, lymphoid-tissue and other organ-on-a-chip platforms.[6,7,8,9]
Co-culture & Functional Assays
The 2X format makes it simple to fold in treatment solutions, cytokines or test compounds at a final 1X medium.
Metabolic Flux Analysis
A defined glucose, pyruvate and amino-acid background for immunometabolism, isotope tracing and respirometry.
Microscopy & Flow Cytometry
Fewer particles means cleaner fields and fewer debris events in confocal imaging and flow analysis.
Specifications & quality control
Release specifications for DCP-CM-Q2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 2.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] L-Glutamine, [−] Mercaptoethanol |
| Concentration | 2X (mix 1:1 to 1X) |
| Appearance | Red |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| Glucose | 2,000 mg/L (2.0 g/L) |
| L-Glutamine | Not included |
| Sodium Pyruvate | 550 mg/L |
| Sodium Bicarbonate | 2,700 mg/L |
| 2-Mercaptoethanol | Not included |
| HEPES | Not included |
| Phenol Red | 22 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | CO₂-controlled incubator (typically 5% CO₂) after 1:1 dilution; sodium bicarbonate 2,700 mg/L in the 2X, 1,350 mg/L at 1X |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-CM-Q2X is 2X Click's Medium with 2.0 g/L glucose, sodium pyruvate, sodium bicarbonate and Phenol Red. It does not contain L-glutamine, Mercaptoethanol nor HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 42 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 mixing.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 491.060 |
| Disodium hydrogen phosphate | 7558-79-4 | 95.800 |
| Magnesium chloride anhydrous | 7786-30-3 | 329.800 |
| Magnesium sulphate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 120.000 |
| Sodium bicarbonate | 144-55-8 | 2700.000 |
| Sodium chloride | 7647-14-5 | 16000.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 60.000 |
| L-Alanine | 56-41-7 | 71.200 |
| L-Arginine hydrochloride | 1119-34-2 | 635.000 |
| L-Asparagine anhydrous | 70-47-3 | 105.600 |
| L-Aspartic acid | 56-84-8 | 106.400 |
| L-Cystine dihydrochloride | 30925-07-6 | 156.400 |
| L-Glutamic acid | 56-86-0 | 117.600 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 209.400 |
| L-Isoleucine | 73-32-5 | 260.000 |
| L-Leucine | 61-90-5 | 260.000 |
| L-Lysine hydrochloride | 657-27-2 | 362.400 |
| L-Methionine | 63-68-3 | 75.000 |
| L-Phenylalanine | 63-91-2 | 160.000 |
| L-Proline | 147-85-3 | 92.000 |
| L-Serine | 56-45-1 | 84.000 |
| L-Threonine | 72-19-5 | 240.000 |
| L-Tryptophan | 73-22-3 | 50.000 |
| L-Tyrosine disodium salt dihydrate | 122666-87-9 | 237.80 |
| L-Valine | 72-18-4 | 230.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Calcium pantothenate | 137-08-6 | 4.000 |
| Choline chloride | 67-48-1 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Niacinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| myo-Inositol | 87-89-8 | 8.000 |
| OTHERS | ||
| Adenosine | 58-61-7 | 50.000 |
| Cytosine | 71-30-7 | 50.000 |
| Glucose | 50-99-7 | 2000.000 |
| Guanosine | 118-00-3 | 50.000 |
| Phenol Red | 34487-61-1 | 22.000 |
| Sodium pyruvate | 113-24-6 | 550.000 |
| Uridine | 58-96-8 | 50.000 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-CM-Q2X compares
Same EHAA chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-CM-Q2X (FluxMPS™) | Conventional Click's Medium, 1X (0.22 µm filtered) | Standard RPMI 1640 (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X Click's (EHAA), L-glutamine- and 2-ME-free | 1X EHAA, often with 2-ME added | General lymphocyte medium |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-CM-Q2X for immune-cell, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on Click's Medium, in vitro immune responses, hybridomas, organ-on-a-chip culture and mycoplasma control.
- Click RE, Benck L, Alter BJ. Immune responses in vitro. I. Culture conditions for antibody synthesis. Cell Immunol. 1972;3(2):264–276.
- Mishell RI, Dutton RW. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967;126(3):423–442. doi:10.1084/jem.126.3.423
- Köhler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975;256(5517):495–497. doi:10.1038/256495a0
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Ingber DE. Human organs-on-chips for disease modelling, drug development and personalized medicine. Nat Rev Genet. 2022;23(8):467–491. doi:10.1038/s41576-022-00466-9
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
- Esch EW, Bahinski A, Huh D. Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov. 2015;14(4):248–260. doi:10.1038/nrd4539