FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Sodium Pyruvate, Phenol Red: 2X Liquid

FluxMPS™ Leibovitz's L-15 Medium with 50mM HEPES w/o Sodium Pyruvate, Phenol Red — 2X Liquid

FluxMPS™ DCP-LL15H-PR2X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) Leibovitz's L-15 2X + 50mM HEPES formulation engineered for primary cells and CO₂-free culture on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (50 mM, pKa 7.3 at 37°C) provides robust CO₂-independent pH buffering, and D-galactose (1.8 g/L) supplies a defined, glycolysis-neutral carbon source in place of glucose.

• CO₂-independent by design: D-galactose energy source plus high amino-acid buffering capacity — no gas supplementation required for open-bench and chip-loading workflows
• 50 mM HEPES (pKa 7.3 at 37°C) provides robust pH stability during extended open-stage imaging and dissociation/transport procedures
• Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II (Polish)
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
• Sodium pyruvate and phenol red both excluded by formulation design — autofluorescence-reduced base for live imaging; researcher adds pyruvate if required by their assay
• Manufactured under an ISO 13485:2016 quality management system; final QA at Diagnocine, Totowa, NJ, USA
• pH 7.4 (USP <791>); osmolality 620–680 mOsm/kg H₂O (2X concentrated formulation)

Engineered where standard media fails

Conventional 0.22 µm–filtered Leibovitz's formulations pass subvisible particulates, mycoplasma-scale organisms, and endotoxin fragments that interfere with CO₂-free pH maintenance and live imaging clarity. FluxMPS™ is built to address these gaps.

Quadruple-stage filtration system

Four serial filtration passes — two dedicated prefilter/final-filter pairs — reach a final 0.04 µm polish. Each 0.04 µm final filter has its own dedicated 0.1 µm prefilter ahead of it, giving full redundancy across the train.

1. 1

   0.1 µm Prefiltration I

   Removes large aggregates and cell debris; provides mycoplasma-retentive risk mitigation (0.1 µm, the grade validated against A. laidlawii-scale organisms) ahead of the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that a standard 0.22 µm filter does not.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, providing mycoplasma-retentive risk mitigation and protecting the second 0.04 µm cartridge; second-pass redundancy.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish.

CO₂-free live imaging and primary cell applications

FluxMPS™ DCP-LL15H-PR2X — Leibovitz's L-15 2X + 50mM HEPES — delivers 0.04 µm filtered purity for primary cell and OoC applications outside the CO₂ incubator.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated perfusion systems.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix below. CoA: support@diagnocine.com.

Full composition (mg/L)

Leibovitz's L-15 2X + 50mM HEPES: 34 ingredients verified per lot, with CAS numbers for raw-material traceability. This formulation uses D-galactose (not glucose) as the primary carbon source and high amino-acid concentrations for CO₂-free pH buffering, is 2X concentrated, and includes 50 mM HEPES for enhanced pH stability during prolonged open-stage procedures.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

How DCP-LL15H-PR2X compares

FluxMPS™ DCP-LL15H-PR2X vs. conventional 0.22 µm–filtered Leibovitz's and standard DMEM/RPMI formulations.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-LL15H-PR2X — Leibovitz's L-15 2X + 50mM HEPES.

Supporting literature

Key publications supporting Leibovitz's L-15 2X + 50mM HEPES in CO₂-free live-cell imaging, primary cell culture, and OoC applications.

1. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180. doi:10.1093/oxfordjournals.aje.a120330
2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
7. Ludolph I, et al. Comparison of L-15 medium and standard CO₂-buffered media for open-air live-cell microscopy. Microsc Res Tech. 2019;82:1401–1409. doi:10.1002/jemt.23291
8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j