FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth w/o Sodium Bicarbonate(TPB): 1X Liquid

FluxMPS™ Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth w/o Sodium Bicarbonate (TPB): 1X Liquid

ureFluxMPS™ DCP-GMEM-BN1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Glasgow's Minimum Essential Medium (GMEM) formulated with high glucose and Tryptose Phosphate Broth (TPB), without sodium bicarbonate. It is engineered for microfluidic channels, organ-on-a-chip (OoC) and microphysiological system (MPS) platforms requiring ultra-low particulate and endotoxin backgrounds.

• High-glucose (4500 mg/L / 4.5 g/L) GMEM base supplemented with Tryptose Phosphate Broth (2950 mg/L), commonly used for BHK-21 and other fastidious cell lines
• Bicarbonate-free formulation — buffering strategy selectable by the end user for open or closed culture systems
• Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
• pH 7.4, manufactured under an ISO 13485:2016 quality management system
• 30 verified ingredients across inorganic salts, amino acids, vitamins and TPB, with CAS traceability
• Custom pH, glucose, salts and nutrient modifications available on request

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and endotoxin fragments that confound sensitive on-chip assays.

Quadruple-stage filtration system

Four filtration passes — two paired 0.1 µm prefilter / 0.04 µm final-filter stages, each 0.04 µm final filter protected by its own dedicated 0.1 µm prefilter — reach a 0.04 µm final pore size, well below the 0.22 µm typical of conventional cell culture media.

1. 1

   0.1 µm Prefiltration I

   Removes large particulate, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that a 0.22 µm filter passes.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

OoC and MPS applications

FluxMPS™ DCP-GMEM-BN1X delivers Microfluidics Suitable purity for organ-on-a-chip and microfluidic applications.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) MPS Grade variant is available on request for automated bioreactors and robotic perfusion systems requiring the finest available cut-off.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates that foul automated systems
• Valve & Sensor Protection: Reduces micro-fouling in automated perfusion and recirculation systems
• Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. Certificate of Analysis available: support@diagnocine.com.

Full composition (mg/L)

Glasgow's Minimum Essential Medium (GMEM), High Glucose with Tryptose Phosphate Broth w/o Sodium Bicarbonate: 1X Liquid — 30 ingredients verified per lot with CAS numbers for raw-material traceability. All ingredients from the original formulation are preserved exactly.

ISO 13485 manufacturing & compliance

Every batch of FluxMPS™ DCP-GMEM-BN1X is manufactured under a certified ISO 13485:2016 QMS with full lot traceability and multi-parameter QC release testing.

How DCP-GMEM-BN1X compares

FluxMPS™ versus conventional 0.22 µm filtered media for OoC and MPS applications.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-GMEM-BN1X and its use in OoC and MPS applications.

Supporting literature

Curated peer-reviewed references relevant to OoC and MPS applications and FluxMPS™ ultra-filtered cell culture media.

1. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nature Biotechnology. 2014;32(8):760–772.doi:10.1038/nbt.2989
2. Sackmann EK, Fulton AL, Beebe DJ. The present and future role of microfluidics in biomedical research. Nature. 2014;507(7491):181–189.doi:10.1038/nature13118
3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662–1668.doi:10.1126/science.1188302
4. Ingber DE. Is it Time for Reviewer 3 to Request Human Organ Chip Experiments Instead of Animal Validation Studies? Advanced Science. 2020;7(22):2002162.doi:10.1002/advs.202002162
5. Maoz BM, et al. A linked organ-on-chip model of the human neurovascular unit reveals the metabolic landscape of brain disease. Nature Biotechnology. 2018;36:865–874.doi:10.1038/nbt.4226
6. Bhise NS, et al. A liver-on-a-chip platform with bioprinted hepatic spheroids. Biofabrication. 2016;8(1):014101.doi:10.1088/1758-5090/8/1/014101
7. Luni C, Serena E, Elvassore N. Human-on-chip for therapy development and fundamental science. Current Opinion in Biotechnology. 2014;25:45–50.doi:10.1016/j.copbio.2013.08.015
8. Erickson KA, Bhansali S. Mycoplasma contamination in cell cultures: a survey of incidence and approaches to prevention. Journal of the Association for Laboratory Automation. 2012;17(5):346–354.doi:10.1177/2211068212456089
9. van Duinen V, et al. Microfluidic 3D cell culture: from tools to tissue models. Current Opinion in Biotechnology. 2015;35:118–126.doi:10.1016/j.copbio.2015.05.002
10. Stanbridge E. Mycoplasmas and cell cultures. Bacteriological Reviews. 1971;35(2):206–227.doi:10.1128/br.35.2.206-227.1971