FluxMPS™ Basal Medium Eagle (BME), 50mM HEPES w/o Sodium Bicarbonate: 2X Liquid
FluxMPS™ Basal Medium Eagle (BME), 50mM HEPES w/o Sodium Bicarbonate: 2X Liquid
Double-strength (2X) Basal Medium Eagle, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a 50 mM HEPES buffer, bicarbonate-free, it is made for plaque-assay overlays, routine culture and microfluidic perfusion — approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: mix 1:1 with a nutrient-free diluent or overlay solution for a final 1X medium
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Phenol Red, [+] Earle's Salts, [+] 50mM HEPES; [−] Sodium Pyruvate, [−] Sodium Bicarbonate
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-Glucose2,000 mg/L
- L-Glutamine584 mg/L
- Sodium PyruvateNot included
- Buffer50 mM HEPES (no NaHCO₃)
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8 °C, protect from light
- Shelf Life12 months
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional BME is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that cloud overlays, settle in microchannels and add noise to optical readouts.[10,12] FluxMPS™ BME keeps Eagle's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and overlays.
Total metabolic control
Defined 2,000 mg/L D-glucose and 584 mg/L L-glutamine in the 2X (half that at 1X), with no sodium pyruvate, for a known carbon-source baseline.
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in plaque counting, confocal imaging and on-chip sensing.
Lean, defined formulation
30 components — Eagle's essential amino acids, vitamins, inorganic salts and glucose — a minimal base you supplement for your cell type.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-BMEH-B2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X BME polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
-
02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
-
03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
-
04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ BME is approximately 5× cleaner than conventional 0.22 µm-filtered BME, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-BMEH-B2X
Where FluxMPS™ BME fits
Basal Medium Eagle (BME) is a foundational cell culture medium developed by Harry Eagle to support mammalian cell cultivation.[1,2] Its formulation provides essential amino acids, vitamins, inorganic salts, and glucose. When supplemented appropriately, BME supports monolayer growth of various normal and transformed cells, including HeLa cells, mouse fibroblast L cells, and primary mammalian cultures.[3,6] Supplementation should be selected according to the requirements of the cell type and application.
Basal Medium Eagle (BME), 2X Liquid, is a double-strength format intended for applications requiring dilution during medium preparation. Mixing equal volumes of 2X BME and a compatible nutrient-free diluent or overlay solution produces a final 1X basal medium concentration, helping prevent dilution below the intended working strength. This format is useful in protocols that specify double-strength medium for preparing semisolid overlays, such as agarose overlays used in viral plaque assays.[4,5] Use BME only where the protocol specifies or validates its suitability, and follow the supplier’s instructions for dilution and supplementation.
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Plaque Assays & Agarose Overlays
The 2X format mixes 1:1 with molten agarose or other overlay solutions so the final overlay sits at 1X working strength.[4,5]
Micro Physiological System (MPS) & Chip
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms and lab-on-a-chip assays.[7,8,9]
Warburg Effect & Metabolic Research
A defined glucose baseline (1 g/L at 1X working strength) for glycolysis and metabolic-shift studies in transformed lines such as HeLa.[2,6]
Fibroblasts & Primary Mammalian Cells
Supplemented BME supports monolayer growth of normal cells, mouse fibroblast L cells and primary mammalian cultures.[1,3]
Metabolic Flux Analysis
A lean, pyruvate-free composition gives a simple background for isotope tracing and respirometry.
Microscopy & Optical Sensing
Fewer particles means cleaner fields for plaque counting, confocal imaging and on-chip biosensors.
Specifications & quality control
Release specifications for DCP-BMEH-B2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 2.0 g/L Glucose, [+] L-Glutamine, [+] Phenol Red, [+] 50mM HEPES; [−] Sodium Pyruvate, [−] Sodium Bicarbonate |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Red |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | 2,000 mg/L (2.0 g/L) |
| L-Glutamine | 584 mg/L |
| Sodium Pyruvate | Not included |
| Sodium Bicarbonate | Not included |
| HEPES | 11,916 mg/L (50 mM) |
| Phenol Red | 22 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | Not required for HEPES buffering; add sodium bicarbonate if the final 1X medium will be held in 5% CO₂ |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-BMEH-B2X is 2X Basal Medium Eagle with 2.0 g/L glucose, L-glutamine, Phenol Red and 50mM HEPES. It does not contain sodium pyruvate nor sodium bicarbonate. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 30 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 530.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Sodium chloride | 7647-14-5 | 13600.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 244.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Arginine hydrochloride | 1119-34-2 | 42.200 |
| L-Cystine dihydrochloride | 30925-07-6 | 31.300 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride | 5934-29-2 | 21.000 |
| L-Isoleucine | 73-32-5 | 52.400 |
| L-Leucine | 61-90-5 | 52.400 |
| L-Lysine hydrochloride | 657-27-2 | 72.960 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 33.000 |
| L-Threonine | 72-19-5 | 47.600 |
| L-Tryptophan | 73-22-3 | 8.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 51.900 |
| L-Valine | 72-18-4 | 46.800 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 2.000 |
| D-Biotin | 58-85-5 | 2.000 |
| D-Ca-Pantothenate | 137-08-6 | 2.000 |
| Folic acid | 59-30-3 | 2.000 |
| Nicotinamide | 98-92-0 | 2.000 |
| Pyridoxal hydrochloride | 65-22-5 | 2.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 2.000 |
| i-Inositol | 87-89-8 | 4.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 2000.000 |
| Phenol Red | 34487-61-1 | 22.000 |
| HEPES | 7365-45-9 | 11916.000 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-BMEH-B2X compares
Same Eagle's chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-BMEH-B2X (FluxMPS™) | Conventional BME, 1X (0.22 µm filtered) | Conventional 2X medium (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X BME with 50 mM HEPES; bicarbonate- and pyruvate-free | 1X BME, ready to use | Double-strength, for overlays |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-BMEH-B2X in plaque assays, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on Basal Medium Eagle, plaque-assay overlays, organ-on-a-chip culture, buffering and mycoplasma control.
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Eagle H. The specific amino acid requirements of a human carcinoma cell (strain HeLa) in tissue culture. J Exp Med. 1955;102(1):37–48. doi:10.1084/jem.102.1.37
- Eagle H. Amino acid metabolism in mammalian cell cultures. Science. 1959;130(3373):432–437. doi:10.1126/science.130.3373.432
- Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99(2):167–182. doi:10.1084/jem.99.2.167
- Baer A, Kehn-Hall K. Viral concentration determination through plaque assays: using traditional and novel overlay systems. J Vis Exp. 2014;(93):e52065. doi:10.3791/52065
- Scherer WF, Syverton JT, Gey GO. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J Exp Med. 1953;97(5):695–710. doi:10.1084/jem.97.5.695
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029