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- FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 30mM HEPES w/o L-Glutamine: 2X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 30mM HEPES w/o L-Glutamine: 2X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium/Ham's F-12 Nutrient Mixture (DMEM/F-12) (3:1) with 30mM HEPES w/o L-Glutamine: 2X Liquid
Double-strength (2X) DMEM/F-12 (3:1) without added trace elements and with 30 mM HEPES (15 mM at 1X) — for keratinocyte, epithelial-cell and reconstructed skin-model research and custom media development, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate + 30 mM HEPES dual buffer, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: dilute to 1X or mix with an equal volume of a compatible solution, cell suspension or hydrogel
- 3:1 DMEM-to-Ham's F-12 ratio, the classic base for keratinocyte and skin-model research
- No added trace elements, so you control trace-element supplementation
- 30 mM HEPES (15 mM at 1X) for CO₂-independent pH stability during handling
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] 7.65 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 30mM HEPES; [−] L-Glutamine, [−] Trace Elements
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-Glucose7,651 mg/L
- L-GlutamineNot included
- Sodium Pyruvate55.05 mg/L
- BufferNaHCO₃ 6,140 mg/L + 30 mM HEPES
- Phenol Red24.47 mg/L
- AppearanceRed
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.
Total metabolic control
Defined 7,651 mg/L D-glucose, no L-glutamine and 55.05 mg/L sodium pyruvate in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.
Rich DMEM/F-12 nutrient base
51 components at double strength, including vitamin B12, linoleic acid, lipoic acid, putrescine, hypoxanthine and thymidine from Ham's F-12.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-DMF12H-QT2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
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01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
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02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
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03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
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04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-DMF12H-QT2X
Where FluxMPS™ DMEM fits
Dulbecco’s Modified Eagle Medium/Ham’s F-12 Nutrient Mixture (DMEM/F-12) (3:1) combines three parts DMEM with one part Ham’s F-12, providing a basal mixture of amino acids, vitamins, inorganic salts, glucose, and other nutrients for in vitro cell-culture research.[1,2,3] With appropriate supplementation, the 3:1 blend is used in keratinocyte, epithelial-cell, and reconstructed skin-model research.[4]
For a formulation manufactured without the designated trace-element ingredients, this modified basal medium provides a starting point for investigating trace-element requirements and developing customized supplementation strategies. Cell-specific supplements and experimental validation remain necessary; omission of essential micronutrients should not be assumed to improve cell growth.
Dulbecco’s Modified Eagle Medium/Ham’s F-12 Nutrient Mixture (DMEM/F-12) (3:1), 2X, Without Trace Elements is a double-strength version of the corresponding 1X basal formulation, retaining the same 3:1 DMEM-to-Ham’s-F-12 ratio. Its purpose is to compensate for dilution during preparation of customized research media or compatible semisolid and hydrogel culture systems—a use of concentrated basal medium demonstrated in agarose-based cell-culture research. When combined with an equal volume of a compatible solution that contributes none of the basal-medium ingredients, a 2X formulation provides those ingredients at a final 1X concentration. Final pH, osmolality, buffering, supplementation, and matrix compatibility must still be established for the intended research system. The 30 mM HEPES (15 mM at 1X) adds CO₂-independent buffering for handling outside the incubator.[6]
It is useful for:
- In vitro keratinocyte and epithelial-cell research.
- Reconstructed skin-model and epidermal-equivalent research.
- Investigating trace-element requirements in cultured cells.
- Developing customized supplementation strategies and research media.
- Compatible semisolid, agarose, and hydrogel culture systems.
- Research-use tissue-engineering and organ-on-a-chip studies.
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Keratinocyte & Skin Models
The 3:1 DMEM/Ham's F-12 blend is the classic base for epidermal keratinocyte culture and reconstructed skin equivalents when supplemented appropriately.[4]
Trace-Element Studies
No added trace elements, so you can titrate selenium, manganese and other trace elements in depletion and supplementation experiments.
Calcium-Regulated Differentiation
Extracellular calcium controls keratinocyte proliferation versus stratification; calcium chloride-free versions of this medium are also available for calcium-switch experiments.[5]
Epithelial & Primary Cells
Supports epithelial and other primary cells in defined or low-serum culture after dilution to 1X.[3]
Organ-on-a-Chip & MPS
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]
Microscopy & Optical Sensing
Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.
Specifications & quality control
Release specifications for DCP-DMF12H-QT2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 7.65 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 30mM HEPES; [−] L-Glutamine, [−] Trace Elements |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Red |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | 7,651 mg/L (7.65 g/L; 3.83 g/L at 1X) |
| L-Glutamine | Not included |
| Sodium Pyruvate | 55.05 mg/L |
| Sodium Bicarbonate | 6,140 mg/L |
| Phenol Red | 24.47 mg/L |
| Calcium Chloride | 419.55 mg/L (dihydrate) |
| HEPES | 7,149 mg/L (30 mM; 15 mM at 1X) |
| Added trace elements | Not included |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | CO₂-controlled incubator after dilution to 1X; sodium bicarbonate 6,140 mg/L in the 2X, 3,070 mg/L at 1X; 30 mM HEPES (15 mM at 1X) steadies pH during handling |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-DMF12H-QT2X is 2X DMEM/F-12 (3:1) with 7.65 g/L glucose, sodium pyruvate, sodium bicarbonate, Phenol Red and 30 mM HEPES. It does not contain L-glutamine nor added trace elements. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 51 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 419.550 |
| Copper sulphate pentahydrate | 7758-99-8 | 0.001250 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 71.020 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.150 |
| Ferrous sulphate heptahydrate | 7782-63-0 | 0.418 |
| Magnesium chloride anhydrous | 7786-30-3 | 28.824 |
| Magnesium sulphate anhydrous | 7487-88-9 | 146.580 |
| Potassium chloride | 7447-40-7 | 712.000 |
| Sodium bicarbonate | 144-55-8 | 6140.000 |
| Sodium chloride | 7647-14-5 | 13399.500 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 163.500 |
| Zinc sulphate heptahydrate | 7446-20-0 | 0.430 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 48.760 |
| L-Alanine | 56-41-7 | 4.454 |
| L-Arginine hydrochloride | 1119-34-2 | 231.040 |
| L-Asparagine anhydrous | 70-47-3 | 7.504 |
| L-Aspartic acid | 56-84-8 | 6.650 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 17.560 |
| L-Cystine dihydrochloride | 30925-07-6 | 93.854 |
| L-Glutamic acid | 56-86-0 | 7.350 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 73.480 |
| L-Isoleucine | 73-32-5 | 159.470 |
| L-Leucine | 61-90-5 | 164.050 |
| L-Lysine hydrochloride | 657-27-2 | 237.250 |
| L-Methionine | 63-68-3 | 47.250 |
| L-Phenylalanine | 63-91-2 | 101.480 |
| L-Proline | 147-85-3 | 17.250 |
| L-Serine | 56-45-1 | 68.250 |
| L-Threonine | 72-19-5 | 148.450 |
| L-Tryptophan | 73-22-3 | 25.020 |
| L-Tyrosine disodium salt dihydrate | 122666-87-4 | 159.590 |
| L-Valine | 72-18-4 | 146.850 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 12.980 |
| D-Biotin | 58-85-5 | 0.003650 |
| D-Ca-Pantothenate | 137-08-6 | 6.240 |
| Folic acid | 59-30-3 | 6.660 |
| Niacinamide | 98-92-0 | 6.000 |
| Pyridoxine hydrochloride | 58-56-0 | 6.030 |
| Riboflavin | 83-88-5 | 0.618 |
| Thiamine hydrochloride | 67-03-8 | 6.170 |
| Vitamin B12 | 68-19-9 | 0.680 |
| myo-Inositol | 87-89-8 | 19.800 |
| OTHERS | ||
| DL-Thioctic acid | 1077-28-7 | 0.106 |
| Hypoxanthine sodium salt | 45738-97-4 | 2.400 |
| D-Glucose | 50-99-7 | 7651.000 |
| Linoleic acid | 60-33-3 | 0.042 |
| Putrescine dihydrochloride | 333-93-7 | 0.080 |
| Phenol Red | 34487-61-1 | 24.470 |
| Sodium pyruvate | 113-24-6 | 55.050 |
| Thymidine | 50-89-5 | 0.366 |
| HEPES | 7365-45-9 | 7149.000 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-DMF12H-QT2X compares
Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-DMF12H-QT2X (FluxMPS™) | Conventional DMEM/F-12, 1X (0.22 µm filtered) | Conventional 2X medium (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X DMEM/F-12 (3:1) + 30 mM HEPES; free of L-Glutamine and trace elements | 1X DMEM/F-12, ready to use | Double-strength, general purpose |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-DMF12H-QT2X for semisolid, 3D, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on DMEM and Ham's F-12, keratinocyte culture and calcium regulation, HEPES buffering, organ-on-a-chip culture and mycoplasma control.
- Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53(2):288–293. doi:10.1073/pnas.53.2.288
- Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975;6(3):331–343. doi:10.1016/S0092-8674(75)80001-8
- Hennings H, Michael D, Cheng C, Steinert P, Holbrook K, Yuspa SH. Calcium regulation of growth and differentiation of mouse epidermal cells in culture. Cell. 1980;19(1):245–254. doi:10.1016/0092-8674(80)90406-7
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
- Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029
