Which CMRL 1066 variant should I choose?

The family covers combinations of L-Glutamine, Sodium Bicarbonate, and Phenol Red, plus a specialty formulation without Pyridoxal hydrochloride. Use the supplement matrix to check the box for each supplement required, press Search, and the matching row will highlight so you can click through via the catalog number link or the View button.

Why does CMRL 1066 contain sodium acetate?

Sodium acetate (50 mg/L) is a distinctive feature absent from most other classical media, providing an additional short-chain carbon source and contributing to buffering capacity.

Can CMRL 1066 be customized beyond the listed variants?

Yes. Standard concentration is 1X with 1.0 g/L glucose. Other concentrations, additions, and modifications are available on request via support at diagnocine dot com.

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FluxMPS™ CMRL 1066 Medium

Q: Is CMRL 1066 used for human pancreatic islet culture?

Yes. CMRL 1066 is a field-standard basal medium for the isolation, processing, culture, and preservation of isolated human pancreatic islets in clinical islet transplantation, closely associated with the Edmonton Protocol.

Q: Was CMRL 1066 originally designed for serum-free culture?

Yes. CMRL 1066 was originally formulated for long-term cultivation of mouse L-strain (Earle's) fibroblasts under serum-free, protein-free conditions, one of the earliest media designed explicitly for protein-free growth.

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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.

verified Developed at Connaught Medical Research Laboratories, Late 1950s

FluxMPS™ CMRL 1066 Medium

CMRL 1066 is a defined cell culture medium originally developed in the late 1950s by Connaught Medical Research Laboratories. It is a less complex and extensively modified version of Medium 199, designed initially for long-term cultivation of mouse L cells without protein supplementation. Although originally formulated for serum-free conditions, CMRL 1066 can support the growth of various cell lines when supplemented with serum. This versatile medium contains a balanced mixture of amino acids, vitamins, inorganic salts, and other nutrients necessary for cell growth. CMRL 1066 has been widely used in research and biotechnology applications, including the culture of human pancreatic islets. Every FluxMPS™ variant is purified through quadruple-stage 0.04 micron filtration, engineered for organ-on-chip (OoC), tissue-on-chip (ToC), and lab-on-chip (LoC) microfluidic culture systems.

9 variants across L-Glutamine, Sodium Bicarbonate, and Phenol Red inclusion/exclusion, plus a specialty formulation without Pyridoxal hydrochloride
58-component chemically defined formulation — one of the most nutritionally complete classical media, including coenzymes and DNA nucleoside precursors absent from DMEM, MEM, and RPMI 1640
A substantially modified and simplified derivative of Medium 199, retaining its nucleic acid derivatives and coenzymes
Field-standard basal medium for human pancreatic islet isolation, culture, and preservation, closely associated with the Edmonton Protocol
Reduced glutathione at approximately 200x the concentration found in Medium 199, supporting intracellular reducing capacity
FluxMPS™ quadruple-stage 0.04 micron filtration — finer than any ready-to-use cell culture media currently available
Available in 500 mL and 1000 mL sizes; 2-8°C storage away from bright light; fully customizable on request (including HEPES buffering)

DCP-CMRL SERIES — 9 VARIANTS

FluxMPS™ CMRL 1066 Medium — 1X Liquid Cell Culture Medium

Concentration1X
Glucose1.0 g/L (5.5 mM), Low Glucose
L-GlutamineConfigurable (+/-); add 2 mM if not in base
Sodium BicarbonateConfigurable (+/-), 2200 mg/L
Phenol RedConfigurable (+/-)
CoenzymesCoA, cocarboxylase (TPP), NAD, NADP, FAD
DNA nucleosidesDeoxyadenosine, deoxycytidine, deoxyguanosine, thymidine
Sizes500 mL / 1000 mL
Storage2-8 C, away from light

RUO Parker et al. 1957 Foundational Customizable

Product Selector

Select Your CMRL 1066 Medium Configuration

Please select the supplement(s) of interest, then click Search. Check the supplement(s) you need below and press Search to instantly highlight every matching variant. Each row links straight through to its product page via the catalog number or the View button.

At-a-glance supplement matrix — 9 variants

At-a-glance supplement matrix — click to view product page. HEPES buffering is available as a customization on request but is not a standard catalog option.

Name	Cat No.	L-Glutamine	Bicarbonate	Phenol Red	Product Page
CMRL	DCP-CMRL1X	check	check	check	Viewarrow_forward
CMRL w/o Glutamine	DCP-CMRL-Q1X	remove	check	check	Viewarrow_forward
CMRL w/o Bicarbonate	DCP-CMRL-B1X	check	remove	check	Viewarrow_forward
CMRL w/o Phenol Red	DCP-CMRL-R1X	check	check	remove	Viewarrow_forward
CMRL w/o Glutamine, Bicarbonate	DCP-CMRL-QB1X	remove	remove	check	Viewarrow_forward
CMRL w/o Glutamine, Phenol Red	DCP-CMRL-QR1X	remove	check	remove	Viewarrow_forward
CMRL w/o Bicarbonate, Phenol Red	DCP-CMRL-BR1X	check	remove	remove	Viewarrow_forward
CMRL w/o Glutamine, Bicarbonate, Phenol Red	DCP-CMRL-QBR1X	remove	remove	remove	Viewarrow_forward
CMRL w/o Pyridoxal Hydrochloride (w/o Pyridoxal hydrochloride)	DCP-CMRL-YY1X	check	check	check	Viewarrow_forward

Customization available: All variants above use the standard 1X concentration and 1.0 g/L Glucose. Other concentrations, additions of chemicals, compounds, proteins, supplements (including HEPES buffer), a different pH, and modifications are available on request — contact support@diagnocine.com.

About CMRL 1066

About CMRL 1066 Medium

CMRL 1066 is a chemically defined, nucleoside- and coenzyme-rich cell culture medium developed at the Connaught Medical Research Laboratories (CMRL), University of Toronto, Canada, in the late 1950s. It is a substantially modified and simplified derivative of Medium 199 (Morgan, Morton & Parker, 1950), originally formulated for the long-term cultivation of mouse L-strain (Earle's) fibroblasts under serum-free conditions, making it one of the earliest mammalian cell culture media designed explicitly for protein-free growth. Its distinguishing chemical profile — 58 components including an exceptionally broad vitamin-coenzyme panel, DNA nucleoside precursors, reduced glutathione, glucuronic acid, and Tween 80 — reflects the ambition of its developers to fully replace undefined biological supplements with chemically pure constituents.

Although its serum-free applications are now largely superseded by later defined media, CMRL 1066 has found an enduring and clinically important second life as a standard basal medium for the isolation, culture, and preservation of human pancreatic islets in transplantation, associated with the Edmonton Protocol (Shapiro et al., 2000) and used across international transplant centers.

Origins and Development: The Connaught Laboratories Medium Series

CMRL 1066 did not emerge as a single designed formulation but as the end product of a sustained, iterative research program at the Connaught Medical Research Laboratories, University of Toronto, spanning roughly a decade. The Connaught group, principally Raymond C. Parker, George M. Healy, and their colleagues, had been systematically defining the nutritional requirements of animal cells in tissue culture since the early 1950s. Their progressive medium series included:

Synthetic Medium No. 703 — Healy, Fisher & Parker (1954), Can. J. Biochem. Physiol. 32(3): 327-337; an early synthetic medium testing systematic amino acid and vitamin combinations in stationary culture.
Synthetic Medium No. 858 — Healy, Fisher & Parker (1955), Proc. Soc. Exp. Biol. Med. 89(1): 71-77; a further refinement, expanding the coenzyme content and evaluating growth responses of Earle's L cells.
CMRL 1066 — Parker, R.C., Castor, L.N. & McCulloch, E.A. (1957), "Altered Cell Strains In Continuous Culture: A General Survey," Special Publications of the New York Academy of Sciences, 5: 303-313.

Relationship to Medium 199

CMRL 1066 is explicitly described in its own technical literature as a less complex and extensively modified version of Medium 199. Medium 199, developed by Morgan, Morton & Parker in 1950 (Proc. Soc. Exp. Biol. Med. 73(1): 1-8), was the first comprehensive chemically defined medium and contained over 60 components, including amino acids, vitamins, nucleic acid bases, lipid precursors, and coenzymes. CMRL 1066 retains the Medium 199 philosophy of chemical completeness, specifically preserving the nucleic acid derivatives and coenzymes omitted in the intermediate H597 modification of Medium 199 used for poliovirus vaccine production, while simplifying and adjusting concentrations to sustain L-cell growth.

A particularly notable compositional feature inherited and amplified from Medium 199 is the inclusion of reduced glutathione. CMRL 1066 contains glutathione at approximately 200 times the concentration found in Medium 199 (10 mg/L vs approximately 0.05 mg/L), reflecting the Connaught group's emphasis on intracellular reducing capacity for serum-free maintenance of rapidly dividing cells.

Subsequent CMRL Derivatives

CMRL 1415 — Healy & Parker (1966), J. Cell Biol. 30(3): 531-538. PMID: 5971005. An improved chemically defined medium for newly explanted mouse embryo cells, devised by systematic modification of CMRL 1066; contains 50 components and incorporates galactose, pyruvate, and enhanced phosphate buffering. Omitting sodium bicarbonate yields CMRL-1415-ATM for open-atmosphere use.
CMRL 1969 — Healy, Teleki, Seefried, Walton & Macmorine (1971), Appl. Microbiol. 21(1): 1-5. An improved medium for primary monkey kidney cells and human diploid cell strains, used to advantage for poliovirus production; improvements attributed to free-base amino acids and increased dibasic sodium phosphate.

Composition

The following formulation matches the standard CMRL 1066 vendor composition (HiMedia/CDH and Sigma). All concentrations are in mg/L. The complete formulation contains 58 components and is supplied without L-glutamine (added separately) and in some preparations without phenol red. Per-lot Certificate of Analysis (CoA) requests can be directed to support@diagnocine.com.

Inorganic Salts

Salt	mg/L
Calcium chloride dihydrate (CaCl2 * 2H2O)	265.00
Magnesium sulfate anhydrous (MgSO4)	97.72
Potassium chloride (KCl)	400.00
Sodium acetate anhydrous (CH3COONa)	50.00
Sodium bicarbonate (NaHCO3)	2200.00
Sodium chloride (NaCl)	6800.00
Sodium dihydrogen phosphate anhydrous (NaH2PO4)	122.00

Salt system and buffering: CMRL 1066 uses an Earle's-type salt framework (MgSO4; NaCl as principal osmolyte) rather than the Hanks' BSS dual-magnesium system. A distinctive feature absent from most other classical media is sodium acetate (50 mg/L), which provides an additional short-chain carbon source and contributes to buffering capacity. The NaHCO3 concentration (2200 mg/L) is close to that of RPMI 1640 (2000 mg/L) and lower than IMDM (approximately 3024 mg/L) and DMEM (3700 mg/L); it requires a humidified 5-10% CO2 atmosphere to maintain pH. Phosphate system: A single monobasic phosphate (NaH2PO4) is used, in contrast to the dual monobasic-dibasic phosphate system introduced in CMRL 1415 and CMRL 1969 as an improvement.

Amino Acids

CMRL 1066 contains 20 amino acids in the base (L-glutamine-free) formulation, including the unusual amino acid trans-4-hydroxy-L-proline and a dual cysteine/cystine system; with L-glutamine added separately, the complete medium has 21 amino acids.

Amino Acid	mg/L
Glycine	50.00
L-Alanine	25.00
L-Arginine (free base)	57.87
L-Aspartic acid	30.00
L-Cysteine hydrochloride monohydrate	260.00
L-Cystine	20.00
L-Glutamic acid	75.00
L-Histidine hydrochloride monohydrate	20.00
L-Isoleucine	20.00
L-Leucine	60.00
L-Lysine hydrochloride	70.00
L-Methionine	15.00
L-Phenylalanine	25.00
L-Proline	40.00
L-Serine	25.00
L-Threonine	30.00
L-Tryptophan	10.00
L-Tyrosine (disodium salt)	40.00
L-Valine	25.00
Trans-4-hydroxy-L-proline	10.00
L-Glutamine (added separately)	292 mg/L

Vitamins

Vitamin	mg/L
D-Biotin	0.010
Folic acid	0.010
L-Ascorbic acid (sodium salt)	50.000
Niacinamide (nicotinamide)	0.025
Nicotinic acid	0.025
Pyridoxal hydrochloride	0.025
Pyridoxine hydrochloride	0.025
Riboflavin	0.010
Thiamine hydrochloride	0.010
myo-Inositol	0.050
Choline chloride	0.500
D-Calcium pantothenate	0.010
p-Aminobenzoic acid (PABA)	0.050

Ascorbic acid (50 mg/L): the highest-concentration vitamin in CMRL 1066, absent from DMEM and MEM; antioxidant and cofactor for prolyl/lysyl hydroxylases in collagen biosynthesis. PABA (0.050 mg/L): a B-complex factor retained from Medium 199. Dual vitamin B6: both pyridoxal HCl and pyridoxine HCl at 0.025 mg/L each. Biotin (0.010 mg/L): present, unlike DMEM and Click's EHAA.

Coenzymes and Metabolic Cofactors

These components are absent from DMEM, MEM, RPMI 1640, and Click's EHAA.

Coenzyme / Cofactor	mg/L
Coenzyme A sodium salt (CoA * Na)	2.500
Cocarboxylase (thiamine pyrophosphate, TPP)	1.000
Beta-NAD (nicotinamide adenine dinucleotide, oxidized)	7.000
Beta-NADP sodium salt (NADP)	1.000
FAD disodium salt (flavin adenine dinucleotide)	0.106

Metabolic rationale: CoA (fatty acid synthesis, TCA cycle, amino acid catabolism), cocarboxylase/TPP (pyruvate and alpha-ketoglutarate dehydrogenase), beta-NAD and beta-NADP (central redox coenzymes), and FAD (respiratory chain, flavoenzymes) were included as preformed coenzymes to bypass potential rate-limiting biosynthetic steps under serum-free conditions.

DNA Nucleoside Precursors

Nucleoside	mg/L
2'-Deoxyadenosine	10.00
2'-Deoxycytidine hydrochloride	11.60
2'-Deoxyguanosine	10.00
5-Methyldeoxycytidine	0.10
Thymidine	10.00

Unlike Click's EHAA (which provides ribonucleoside precursors), CMRL 1066 provides deoxyribonucleoside precursors — the direct building blocks of DNA synthesis. (Some vendors list the methylated component as "5-methylcytosine HCl" rather than 5-methyldeoxycytidine; the concentration is 0.1 mg/L either way.)

Other Components

Component	mg/L
D-Glucose anhydrous	1000.00
L-Glutathione (reduced)	10.00
D-Glucuronic acid sodium salt	3.88
Uridine-5'-triphosphate sodium salt (UTP * Na)	1.00
Cholesterol	0.20
Tween 80 (polyoxyethylene sorbitan monooleate)	5.00
Phenol red sodium salt	approximately 20-21

Reduced glutathione (10 mg/L): approximately 200x the level in Medium 199; principal intracellular non-protein thiol and the central mechanism by which CMRL 1066 maintains viable cells under protein-free conditions. Tween 80 (5 mg/L): non-ionic surfactant to solubilize cholesterol and deliver lipids, substituting for serum albumin's lipid-carrier role. Cholesterol (0.20 mg/L): direct lipid supplement. D-Glucuronic acid (3.88 mg/L): glycosaminoglycan biosynthesis precursor. UTP (1 mg/L): preformed ribonucleotide triphosphate for RNA biosynthesis.

Complete Composition Summary

Category	Details
Total components	58
Salt system	Earle's-type (MgSO4; NaCl; single monobasic NaH2PO4); distinctive sodium acetate (50 mg/L)
Amino acids	20 in base + L-glutamine added separately = 21 total (including trans-4-hydroxy-L-proline and dual cysteine/cystine)
Classical vitamins	13 — including biotin, ascorbic acid, PABA, dual B6 (pyridoxal + pyridoxine); no B12
Coenzymes	CoA, cocarboxylase (TPP), beta-NAD (7.0), beta-NADP (1.0), FAD; absent from other classical media
DNA nucleosides	2'-deoxyadenosine, 2'-deoxycytidine HCl, 2'-deoxyguanosine, 5-methyldeoxycytidine, thymidine
Glucose	1000 mg/L (5.5 mM); low glucose
Glutathione (reduced)	10 mg/L — approximately 200x Medium 199
Lipid supplements	Cholesterol 0.20 mg/L + Tween 80 5 mg/L
NaHCO3	2200 mg/L (some vendors 2750); requires 5-10% CO2
pH	7.4
Osmolality	300-340 mOsm/kg H2O
L-Glutamine	Not in base; add 10 mL of 200 mM stock per liter (= 2 mM final)
Serum supplement	Serum-free in original design; when supplemented, horse or calf serum is classically preferred for monkey kidney and many diploid lines

Comparison

Media Lineage Comparison

Feature	CMRL 1066	DMEM (High Glucose)
Parent medium	Medium 199 (modified)	BME (modified)
Developer	Parker et al., CMRL, 1957	Dulbecco & Freeman, 1959
Total components	58	approximately 30
Amino acids (base)	20 (+glutamine = 21; includes hydroxyproline; dual Cys/Cystine)	15
Biotin	Yes	No
Ascorbic acid	Yes, 50 mg/L	No
PABA	Yes	No
Coenzymes (CoA, TPP, NAD, NADP, FAD)	Yes	No
DNA deoxyribonucleosides	Yes (5 types)	No
Glutathione	Yes, 10 mg/L	No
Lipid supplements	Cholesterol + Tween 80	No
Sodium acetate	Yes, 50 mg/L	No
Glucose	1000 mg/L	4500 mg/L
NaHCO3	2200 mg/L	3700 mg/L
Serum-free design	Yes (original intent)	No
pH	approximately 6.8-7.6	approximately 7.2
Osmolality	300-340 mOsm/kg	approximately 320-355 mOsm/kg

Validated Applications

Validated Cell Types and Applications

Founding Applications (Serum-Free)

Mouse L-Strain (Earle's) Fibroblasts

CMRL 1066 was optimized for long-term maintenance of monolayer L-cell cultures under serum-free, protein-free conditions; it is the cell line on which the entire CMRL series was evaluated.

Monkey Kidney Cells (Cloning)

CMRL 1066 is documented as a medium for cloning monkey kidney cells under defined conditions.

Extended Applications (With Serum)

General Fibroblast-Like & Permanent Cell Lines

With horse or calf serum (10%), CMRL 1066 supports a wide variety of mammalian cell types, particularly fibroblast-like adherent lines, and has been used with human and monkey cells.

Bovine Uterine & Oviduct Epithelial Cells

In a published comparative study, these cells showed the highest 8-day growth rates in CMRL 1066 versus Medium 199, MEM, Menezo's B2, and Ham's F-12.

Mouse Myeloma Tumor Stem Cells

CMRL 1066 was reported to contain an unidentified labile factor required by myeloma cells but not hematopoietic cells in soft-agar colony assays.

Borrelia burgdorferi (BSK Medium)

CMRL 1066 (10x) is a principal ingredient of Barbour-Stoenner-Kelly (BSK/BSK-II) medium, the standard medium for cultivating the Lyme disease spirochete.

Principal Contemporary Application: Human Pancreatic Islet Culture

CMRL 1066 (supplemented) is a field-standard basal medium for the isolation, processing, culture, and preservation of isolated human pancreatic islets in clinical islet transplantation. It is closely associated with the Edmonton Protocol (Shapiro, A.M.J. et al., 2000, N. Engl. J. Med. 343(4): 230-238). Note, however, that the original Edmonton Protocol transplanted freshly isolated islets (typically within approximately 2 hours of isolation), so CMRL 1066 served as the isolation/processing medium rather than a pretransplant culture medium in that protocol; extended islet culture in CMRL 1066 subsequently became standard and is now widely used.

The biochemical rationale for CMRL 1066 in islet work connects directly to its composition:

Ascorbic acid (50 mg/L) — antioxidant protection; role in beta-cell function
Reduced glutathione (10 mg/L) — defense against oxidative stress during isolation
Glucose (5.5 mM) — near-euglycemic, avoiding supra-physiological glucose toxicity
Coenzymes (NAD, CoA, FAD) — support the metabolically active islet endocrine cells
DNA deoxyribonucleosides — support islet cell viability during culture

Standard supplementation for islet culture typically includes 10% FCS or human serum albumin, L-glutamine (2 mM), penicillin, and streptomycin. Enriched protocols build on the CMRL 1066 base with zinc, insulin, transferrin, selenium (ITS; "Memphis media" = CMRL + ITS), sodium pyruvate, and antioxidants. The Edmonton group's international multicenter trial (Shapiro et al., N. Engl. J. Med. 2006; 355(13): 1318-1330) validated the CMRL 1066-based protocol across sites.

Why FluxMPS™

Why FluxMPS™ CMRL 1066 Medium

filter_alt

Quadruple-Stage 0.04 Micron Filtration

Purified to 0.04 microns — finer than any ready-to-use cell culture media currently available — removing the microscopic particulates and protein aggregates that silently block micro-channels.

device_hub

Built for OoC / ToC / LoC Platforms

Engineered from the ground up for Organ-on-Chip, Tissue-on-Chip, and Lab-on-Chip platforms, where the medium itself is part of the instrument and cannot be an afterthought.

water_drop

Particulate & Aggregate Removal

Eliminates particulates and protein aggregates that disrupt laminar flow and generate false biological signals before the media ever reaches the chip.

visibility

Optical Clarity

Supports real-time imaging and integrated biosensing on Organ-on-Chip platforms, where optical clarity of the medium is essential to data quality.

verified_user

FDA-Recognized Physiological Modeling Standards

Formulated to support FDA-recognized physiological modeling standards, providing a validated, reproducible media foundation for regulatory-facing studies.

science

Drug Discovery, Toxicology & Translational Research

A validated foundation for drug discovery, toxicology screening, and translational research, where downstream data must stand up to regulatory scrutiny.

Filtration Technology

Quadruple-Stage Filtration System

Every FluxMPS™ CMRL 1066 Medium variant passes through the same four-stage architecture before reaching your chip.

01

Pre-Filtration Stage 1 0.1 µm

Initial coarse particulate removal.
02

Pre-Filtration Stage 2 0.1 µm

Secondary particulate and aggregate reduction.
03

Sterile Filtration Stage 1 0.04 µm

Fine sterile filtration below standard 0.22 micron practice.
04

Sterile Filtration Stage 2 0.04 µm

Final polish for microfluidic-grade clarity.

Engineered for Flow, Not Just Growth

Every component is optimized for consistent, laminar flow performance across complex micro-channel geometries, capillary-bed and vascular simulations, and long-term automated perfusion studies running continuously for weeks.

0.04µm

Final filtration stage

Total filtration stages

FluxMPS(TM) CMRL 1066 Medium Quadruple-stage filtration system diagram - two 0.1 micron pre-filtration stages followed by two 0.04 micron sterile-filtration stages, engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and lab-on-a-chip microfluidic cell culture media applications by Diagnocine.

FAQ

Frequently Asked Questions

The family covers combinations of L-Glutamine, Sodium Bicarbonate, and Phenol Red, plus a specialty formulation without Pyridoxal hydrochloride. Use the supplement matrix above: check the box for each supplement your protocol requires, press Search, and the matching row (or rows) will highlight in mint green so you can click straight through via the catalog number link or the View button.

CMRL 1066 is a 58-component formulation derived from Medium 199, containing coenzymes (CoA, cocarboxylase/TPP, NAD, NADP, FAD), DNA deoxyribonucleoside precursors, reduced glutathione, biotin, ascorbic acid, PABA, and lipid supplements (cholesterol, Tween 80) that are all absent from DMEM, MEM, and RPMI 1640.

Yes. CMRL 1066 (supplemented) is a field-standard basal medium for the isolation, processing, culture, and preservation of isolated human pancreatic islets in clinical islet transplantation, closely associated with the Edmonton Protocol. Standard supplementation typically includes 10% FCS or human serum albumin, L-glutamine (2 mM), penicillin, and streptomycin.

Yes. CMRL 1066 was originally formulated for the long-term cultivation of mouse L-strain (Earle's) fibroblasts under serum-free, protein-free conditions, making it one of the earliest mammalian cell culture media designed explicitly for protein-free growth. Although originally formulated for serum-free conditions, CMRL 1066 can support the growth of various cell lines when supplemented with serum.

Sodium acetate (50 mg/L) is a distinctive feature absent from most other classical media. It provides an additional short-chain carbon source and contributes to buffering capacity.

Beyond pancreatic islets, CMRL 1066 has been used for monkey kidney cell cloning, general fibroblast-like and permanent cell lines with serum, bovine uterine and oviduct epithelial cells, mouse myeloma tumor stem cells in soft-agar colony assays, and as a principal ingredient (at 10x concentration) of BSK/BSK-II medium for cultivating the Lyme disease spirochete Borrelia burgdorferi.

HEPES is not a standard catalog option for this family, but it is available as a customization on request via support@diagnocine.com.

Yes. Standard concentration is 1X with 1.0 g/L glucose. Other concentrations, additions of chemicals, compounds, proteins, supplements, a different pH, and modifications are available on request via support@diagnocine.com.

Variants are available in 500 mL and 1000 mL sizes. Store at 2-8°C, away from bright light.

References

Verified Bibliography

Parker, R.C., Castor, L.N. & McCulloch, E.A. (1957). Altered Cell Strains In Continuous Culture: A General Survey. Special Publications of the New York Academy of Sciences, 5: 303-313.
Morgan, J.F., Morton, H.J. & Parker, R.C. (1950). Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium. Proc. Soc. Exp. Biol. Med., 73(1): 1-8. DOI: 10.3181/00379727-73-17557
Healy, G.M., Fisher, D.C. & Parker, R.C. (1954). Nutrition of animal cells in tissue culture. IX. Synthetic medium No. 703. Can. J. Biochem. Physiol., 32(3): 327-337.
Healy, G.M., Fisher, D.C. & Parker, R.C. (1955). Nutrition of animal cells in tissue culture. X. Synthetic medium No. 858. Proc. Soc. Exp. Biol. Med., 89(1): 71-77. DOI: 10.3181/00379727-89-21719
Healy, G.M. & Parker, R.C. (1966). An improved chemically defined basal medium (CMRL-1415) for newly explanted mouse embryo cells. J. Cell Biol., 30(3): 531-538. PMID: 5971005. PMCID: PMC2107014. DOI: 10.1083/jcb.30.3.531
Healy, G.M., Teleki, S., Seefried, A.V., Walton, M.J. & Macmorine, H.G. (1971). Improved Chemically Defined Basal Medium (CMRL-1969) for Primary Monkey Kidney and Human Diploid Cells. Appl. Microbiol., 21(1): 1-5. PMCID: PMC377105
Shapiro, A.M.J., Lakey, J.R.T., Ryan, E.A., et al. (2000). Islet Transplantation in Seven Patients with Type 1 Diabetes Mellitus Using a Glucocorticoid-Free Immunosuppressive Regimen. N. Engl. J. Med., 343(4): 230-238. DOI: 10.1056/NEJM200007273430401
Shapiro, A.M.J., et al. (2006). International Trial of the Edmonton Protocol for Islet Transplantation. N. Engl. J. Med., 355(13): 1318-1330. DOI: 10.1056/NEJMoa061267
Yao, T. & Asayama, Y. (2017). Animal-cell culture media: History, characteristics, and current issues. Reproductive Medicine and Biology, 16(2): 99-117. PMC5661806

FluxMPS™ Platform

FluxMPS™ — Precision Cell Culture Media for Microphysiological Systems

Built for the architecture of the future. Not the flask of the past.

Traditional cell culture media were formulated for static well plates and flasks — environments that tolerate impurities, precipitates, and particle loads that would immediately compromise a microfluidic system. FluxMPS™ was designed from the ground up for Organ-on-Chip (OoC), Tissue-on-Chip (ToC), and Lab-on-Chip (LoC) platforms, where the medium itself is part of the instrument.

Purity That Protects Your Platform

FluxMPS™ is purified to 0.04 microns — finer than any ready-to-use cell culture media currently available. At this level, the microscopic particulates and protein aggregates that silently block micro-channels, disrupt laminar flow, and generate false biological signals are eliminated before the media ever reaches your chip.

The result: your platform stays operational, your data stays clean, and your biology drives the result — not your media.

Engineered for Flow, Not Just Growth

The name FluxMPS™ reflects its core design principle. Every component is optimized for consistent, laminar flow performance across:

Complex micro-channel geometries
Capillary-bed and vascular simulations
Long-term automated perfusion studies running continuously for weeks

Zero-clogging performance is not a feature — it is the baseline specification.

Applications & Performance

Application	What FluxMPS™ Delivers
Microfluidics	Stable shear stress; no channel blockage
Metabolic Tracing	Ultra-pure matrix with no contaminant interference
Long-term Perfusion	Consistent formulation stability over weeks of continuous flow
Organ-on-Chip	Optical clarity for real-time imaging and integrated biosensing

Regulatory Foundation

FluxMPS™ is formulated to support FDA-recognized physiological modeling standards, providing a validated, reproducible media foundation for drug discovery, toxicology screening, and translational research. When your downstream data needs to stand up to regulatory scrutiny, your upstream media cannot be an afterthought.

The Bottom Line

Microfluidic platforms are precision instruments. They require precision inputs.

FluxMPS™ is the only ready-to-use cell culture medium engineered specifically to meet that standard — protecting your chip, your cells, and your science.